Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR
The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous...
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MDPI AG
2024-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/1/75 |
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| author | Tigst Demeke |
| author_facet | Tigst Demeke |
| author_sort | Tigst Demeke |
| collection | DOAJ |
| description | The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous detection and quantification of various GMO events. This review covers the various factors to consider for multiplex digital PCR detection and the quantification of GMO events. DNA quality, quantity, and the presence of inhibitors are important factors to consider. Some dPCR instruments allow the use of multiple fluorescent dyes, which facilitates the setup of multiplex dPCR assays. This review focuses on the optimization of multiplex dPCR and describes the multiplex dPCR assays that have been reported for GMO detection. |
| format | Article |
| id | doaj-art-22c30894534249b69fd776b45c861c7a |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-22c30894534249b69fd776b45c861c7a2025-01-10T13:17:43ZengMDPI AGFoods2304-81582024-12-011417510.3390/foods14010075Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCRTigst Demeke0Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3P1N1, CanadaThe number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous detection and quantification of various GMO events. This review covers the various factors to consider for multiplex digital PCR detection and the quantification of GMO events. DNA quality, quantity, and the presence of inhibitors are important factors to consider. Some dPCR instruments allow the use of multiple fluorescent dyes, which facilitates the setup of multiplex dPCR assays. This review focuses on the optimization of multiplex dPCR and describes the multiplex dPCR assays that have been reported for GMO detection.https://www.mdpi.com/2304-8158/14/1/75digital PCRGMO detectionoptimizationmultiplex dPCR |
| spellingShingle | Tigst Demeke Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR Foods digital PCR GMO detection optimization multiplex dPCR |
| title | Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR |
| title_full | Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR |
| title_fullStr | Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR |
| title_full_unstemmed | Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR |
| title_short | Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR |
| title_sort | considerations for the successful detection and quantification of genetically modified events in grain and food samples using multiplex digital pcr |
| topic | digital PCR GMO detection optimization multiplex dPCR |
| url | https://www.mdpi.com/2304-8158/14/1/75 |
| work_keys_str_mv | AT tigstdemeke considerationsforthesuccessfuldetectionandquantificationofgeneticallymodifiedeventsingrainandfoodsamplesusingmultiplexdigitalpcr |