Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain
Abstract Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. Methods A clinical CLE system with fiber probe and...
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| Format: | Article |
| Language: | English |
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Springer
2024-12-01
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| Series: | Journal of Cancer Research and Clinical Oncology |
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| Online Access: | https://doi.org/10.1007/s00432-024-06052-2 |
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| author | Marlen Reichenbach Sven Richter Roberta Galli Matthias Meinhardt Katrin Kirsche Achim Temme Dimitrios Emmanouilidis Witold Polanski Insa Prilop Dietmar Krex Stephan B. Sobottka Tareq A. Juratli Ilker Y. Eyüpoglu Ortrud Uckermann |
| author_facet | Marlen Reichenbach Sven Richter Roberta Galli Matthias Meinhardt Katrin Kirsche Achim Temme Dimitrios Emmanouilidis Witold Polanski Insa Prilop Dietmar Krex Stephan B. Sobottka Tareq A. Juratli Ilker Y. Eyüpoglu Ortrud Uckermann |
| author_sort | Marlen Reichenbach |
| collection | DOAJ |
| description | Abstract Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. Methods A clinical CLE system with fiber probe and 488 nm laser excitation was used to acquire images of tissue autofluorescence. Fresh samples were obtained from routine surgeries (glioblastoma n = 6, meningioma n = 6, brain metastases n = 10, pituitary adenoma n = 2, non-tumor from surgery for the treatment of pharmacoresistant epilepsy n = 2). Additionally, in situ intraoperative label-free CLE was performed in three cases. The autofluorescence images were visually inspected for feature identification and quantification. For reference, tissue cryosections were prepared and further analyzed by label-free multiphoton microscopy and HE histology. Results Label-free CLE enabled the acquisition of autofluorescence images for all cases. Autofluorescent structures were assigned to the cytoplasmic compartment of cells, elastin fibers, psammoma bodies and blood vessels by comparison to references. Sparse punctuated autofluorescence was identified in most images across all cases, while dense punctuated autofluorescence was most frequent in glioblastomas. Autofluorescent cells were observed in higher abundancies in images of non-tumor samples. Diffuse autofluorescence, fibers and round fluorescent structures were predominantly found in tumor tissues. Conclusion Label-free CLE imaging through an approved clinical device was able to visualize the characteristic autofluorescence patterns of human brain tumors and non-tumor brain tissue ex vivo and in situ. Therefore, this approach offers the possibility to obtain intraoperative diagnostic information before resection, importantly independent of any kind of marker or label. |
| format | Article |
| id | doaj-art-22c182248eb14135ac2bdf95b9f1da54 |
| institution | Kabale University |
| issn | 1432-1335 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Cancer Research and Clinical Oncology |
| spelling | doaj-art-22c182248eb14135ac2bdf95b9f1da542025-02-09T12:10:36ZengSpringerJournal of Cancer Research and Clinical Oncology1432-13352024-12-01151111210.1007/s00432-024-06052-2Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brainMarlen Reichenbach0Sven Richter1Roberta Galli2Matthias Meinhardt3Katrin Kirsche4Achim Temme5Dimitrios Emmanouilidis6Witold Polanski7Insa Prilop8Dietmar Krex9Stephan B. Sobottka10Tareq A. Juratli11Ilker Y. Eyüpoglu12Ortrud Uckermann13Department of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenMedical Physics and Biomedical Engineering, Faculty of Medicine, Technische Universität DresdenDepartment of Pathology (Neuropathology), Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenDepartment of Neurosurgery, Faculty of Medicine and University Hospital Carl Gustav Carus, Technische Universität DresdenAbstract Purpose Analysis of autofluorescence holds promise for brain tumor delineation and diagnosis. Therefore, we investigated the potential of a commercial confocal laser scanning endomicroscopy (CLE) system for clinical imaging of brain tumors. Methods A clinical CLE system with fiber probe and 488 nm laser excitation was used to acquire images of tissue autofluorescence. Fresh samples were obtained from routine surgeries (glioblastoma n = 6, meningioma n = 6, brain metastases n = 10, pituitary adenoma n = 2, non-tumor from surgery for the treatment of pharmacoresistant epilepsy n = 2). Additionally, in situ intraoperative label-free CLE was performed in three cases. The autofluorescence images were visually inspected for feature identification and quantification. For reference, tissue cryosections were prepared and further analyzed by label-free multiphoton microscopy and HE histology. Results Label-free CLE enabled the acquisition of autofluorescence images for all cases. Autofluorescent structures were assigned to the cytoplasmic compartment of cells, elastin fibers, psammoma bodies and blood vessels by comparison to references. Sparse punctuated autofluorescence was identified in most images across all cases, while dense punctuated autofluorescence was most frequent in glioblastomas. Autofluorescent cells were observed in higher abundancies in images of non-tumor samples. Diffuse autofluorescence, fibers and round fluorescent structures were predominantly found in tumor tissues. Conclusion Label-free CLE imaging through an approved clinical device was able to visualize the characteristic autofluorescence patterns of human brain tumors and non-tumor brain tissue ex vivo and in situ. Therefore, this approach offers the possibility to obtain intraoperative diagnostic information before resection, importantly independent of any kind of marker or label.https://doi.org/10.1007/s00432-024-06052-2AutofluorescenceIntraoperative imagingLabel-freeBrain tumor recognitionIn situ pathology |
| spellingShingle | Marlen Reichenbach Sven Richter Roberta Galli Matthias Meinhardt Katrin Kirsche Achim Temme Dimitrios Emmanouilidis Witold Polanski Insa Prilop Dietmar Krex Stephan B. Sobottka Tareq A. Juratli Ilker Y. Eyüpoglu Ortrud Uckermann Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain Journal of Cancer Research and Clinical Oncology Autofluorescence Intraoperative imaging Label-free Brain tumor recognition In situ pathology |
| title | Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain |
| title_full | Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain |
| title_fullStr | Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain |
| title_full_unstemmed | Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain |
| title_short | Clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non-tumor brain |
| title_sort | clinical confocal laser endomicroscopy for imaging of autofluorescence signals of human brain tumors and non tumor brain |
| topic | Autofluorescence Intraoperative imaging Label-free Brain tumor recognition In situ pathology |
| url | https://doi.org/10.1007/s00432-024-06052-2 |
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