Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans
This study investigated the lipid-lowering activity and action mechanism of oak essential oil (OEO). OEO had strong in vitro lipid-lowering activity, and its half-maximal inhibitory concentration (IC50) on pancreatic lipase, cholesterol esterase and α-glucosidase were (0.62 ± 0.05), (0.20 ± 0.04) an...
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China Food Publishing Company
2025-07-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-019.pdf |
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| author | WEI Zijie, TAO Zhihua, LI Jingyu, ZHANG Junhao, WU Kegang, CHAI Xianghua, YU Hongpeng, HE Dong, LIU Xiaoli, DUAN Xuejuan, WANG Pingping |
| author_facet | WEI Zijie, TAO Zhihua, LI Jingyu, ZHANG Junhao, WU Kegang, CHAI Xianghua, YU Hongpeng, HE Dong, LIU Xiaoli, DUAN Xuejuan, WANG Pingping |
| author_sort | WEI Zijie, TAO Zhihua, LI Jingyu, ZHANG Junhao, WU Kegang, CHAI Xianghua, YU Hongpeng, HE Dong, LIU Xiaoli, DUAN Xuejuan, WANG Pingping |
| collection | DOAJ |
| description | This study investigated the lipid-lowering activity and action mechanism of oak essential oil (OEO). OEO had strong in vitro lipid-lowering activity, and its half-maximal inhibitory concentration (IC50) on pancreatic lipase, cholesterol esterase and α-glucosidase were (0.62 ± 0.05), (0.20 ± 0.04) and (0.63 ± 0.01) mg/mL, respectively. Enzymatic kinetics and fluorescence emission spectroscopy showed that the inhibition type of OEO on pancreatic lipase was reversible non-competitive inhibition, and the quenching type was static quenching. The in vivo lipid-lowering activity of OEO was also assessed using Caenorhabditis elegans as a model organism. It turned out that OEO increased lipid droplets with a diameter of 0–0.6 μm and decreased lipid droplets with a diameter of 1.2–1.8 μm in high-fat C. elegans; 20 μg/mL of OEO reduced the fat content and total triglyceride level of high-fat C. elegans by 26.46% and 21.35%, respectively. The results of real-time polymerase chain reaction (PCR) showed that OEO significantly up-regulated the relative expression levels of lipid hydrolysis genes (atgl-1 and hosl-1) and fatty acid beta oxidation genes (acs-2), and significantly down-regulated the relative expression levels of fat synthesis genes (fat-5, fat-6, fat-7 and sbp-1). This study indicates that OEO has lipid-lowering activity, providing data support for its application for the alleviation of obesity. |
| format | Article |
| id | doaj-art-22b8ecb94b984a279ddf11ed0fab5e4d |
| institution | DOAJ |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-22b8ecb94b984a279ddf11ed0fab5e4d2025-08-20T03:12:32ZengChina Food Publishing CompanyShipin Kexue1002-66302025-07-01461320517010.7506/spkx1002-6630-20241225-217Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegansWEI Zijie, TAO Zhihua, LI Jingyu, ZHANG Junhao, WU Kegang, CHAI Xianghua, YU Hongpeng, HE Dong, LIU Xiaoli, DUAN Xuejuan, WANG Pingping0(School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China)This study investigated the lipid-lowering activity and action mechanism of oak essential oil (OEO). OEO had strong in vitro lipid-lowering activity, and its half-maximal inhibitory concentration (IC50) on pancreatic lipase, cholesterol esterase and α-glucosidase were (0.62 ± 0.05), (0.20 ± 0.04) and (0.63 ± 0.01) mg/mL, respectively. Enzymatic kinetics and fluorescence emission spectroscopy showed that the inhibition type of OEO on pancreatic lipase was reversible non-competitive inhibition, and the quenching type was static quenching. The in vivo lipid-lowering activity of OEO was also assessed using Caenorhabditis elegans as a model organism. It turned out that OEO increased lipid droplets with a diameter of 0–0.6 μm and decreased lipid droplets with a diameter of 1.2–1.8 μm in high-fat C. elegans; 20 μg/mL of OEO reduced the fat content and total triglyceride level of high-fat C. elegans by 26.46% and 21.35%, respectively. The results of real-time polymerase chain reaction (PCR) showed that OEO significantly up-regulated the relative expression levels of lipid hydrolysis genes (atgl-1 and hosl-1) and fatty acid beta oxidation genes (acs-2), and significantly down-regulated the relative expression levels of fat synthesis genes (fat-5, fat-6, fat-7 and sbp-1). This study indicates that OEO has lipid-lowering activity, providing data support for its application for the alleviation of obesity.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-019.pdfoak essential oil; pancreatic lipase; caenorhabditis elegans; lipid metabolism; gene |
| spellingShingle | WEI Zijie, TAO Zhihua, LI Jingyu, ZHANG Junhao, WU Kegang, CHAI Xianghua, YU Hongpeng, HE Dong, LIU Xiaoli, DUAN Xuejuan, WANG Pingping Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans Shipin Kexue oak essential oil; pancreatic lipase; caenorhabditis elegans; lipid metabolism; gene |
| title | Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans |
| title_full | Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans |
| title_fullStr | Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans |
| title_full_unstemmed | Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans |
| title_short | Lipid-Lowering Activity of Oak Essential Oil and Its Effect on Lipid Metabolism of Caenorhabditis elegans |
| title_sort | lipid lowering activity of oak essential oil and its effect on lipid metabolism of caenorhabditis elegans |
| topic | oak essential oil; pancreatic lipase; caenorhabditis elegans; lipid metabolism; gene |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-13-019.pdf |
| work_keys_str_mv | AT weizijietaozhihualijingyuzhangjunhaowukegangchaixianghuayuhongpenghedongliuxiaoliduanxuejuanwangpingping lipidloweringactivityofoakessentialoilanditseffectonlipidmetabolismofcaenorhabditiselegans |