A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues
Hybrid 3D constructs combining different structural components afford unique opportunities to engineer functional tissues. Creating functional microvascular networks within these constructs is crucial for promoting integration with host vessels and ensuring successful engraftment. Here, we present a...
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Elsevier
2024-12-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424003521 |
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| author | Sara C. Neves Aureliana Sousa Diana S. Nascimento Iasmim D. Orge Sílvia A. Ferreira Carlos Mota Lorenzo Moroni Cristina C. Barrias Pedro L. Granja |
| author_facet | Sara C. Neves Aureliana Sousa Diana S. Nascimento Iasmim D. Orge Sílvia A. Ferreira Carlos Mota Lorenzo Moroni Cristina C. Barrias Pedro L. Granja |
| author_sort | Sara C. Neves |
| collection | DOAJ |
| description | Hybrid 3D constructs combining different structural components afford unique opportunities to engineer functional tissues. Creating functional microvascular networks within these constructs is crucial for promoting integration with host vessels and ensuring successful engraftment. Here, we present a hybrid 3D system in which poly (ethylene oxide terephthalate)/poly (butylene terephthalate) fibrous scaffolds are combined with pectin hydrogels to provide internal topography and guide the formation of microvascular beds. The sequence/method of seeding human endothelial cells (EC) and mesenchymal stromal cells (MSC) into the system had a significant impact on microvessel formation. Optimal results were obtained when EC were directly seeded onto the fibrous scaffold, followed by the addition of hydrogel-embedded MSC. This approach facilitated the development of highly oriented microvascular networks along the fibers. These networks were lumenized, supported by a basement membrane, and stabilized by pericyte-like cells, persisting for at least 28 days in vitro. Furthermore, culture under pro-angiogenic and osteoinductive conditions induced MSC osteogenic differentiation without impairing microvessel formation. Upon subcutaneous implantation in mice, the pre-vascularized constructs were infiltrated by host vessels, and human microvessels were still present after 2 weeks. Overall, the proposed hybrid 3D system, combined with an optimized cell-seeding protocol, offers an effective approach for directing the formation of robust and geometrically oriented microvessels, making it promising for tissue engineering applications. |
| format | Article |
| id | doaj-art-22b7128e996f44b2a47577f9ffc0f8e3 |
| institution | OA Journals |
| issn | 2590-0064 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-22b7128e996f44b2a47577f9ffc0f8e32025-08-20T02:37:43ZengElsevierMaterials Today Bio2590-00642024-12-012910129110.1016/j.mtbio.2024.101291A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissuesSara C. Neves0Aureliana Sousa1Diana S. Nascimento2Iasmim D. Orge3Sílvia A. Ferreira4Carlos Mota5Lorenzo Moroni6Cristina C. Barrias7Pedro L. Granja8i3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; FEUP - Faculdade de Engenharia da Universidade Do Porto, Departamento de Engenharia Metalúrgica e de Materiais, Rua Dr. Roberto Frias, s/n, 4200-465, Porto, Portugal; MERLN - Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, 6229ER Maastricht, the Netherlandsi3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugali3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade Do Porto, R. de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugali3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade Do Porto, R. de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugali3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, PortugalMERLN - Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, 6229ER Maastricht, the NetherlandsMERLN - Institute for Technology-Inspired Regenerative Medicine, Department of Complex Tissue Regeneration, Maastricht University, Universiteitssingel 40, 6229ER Maastricht, the Netherlandsi3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade Do Porto, R. de Jorge Viterbo Ferreira, 228, 4050-313, Porto, Portugal; Corresponding author. i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.i3S - Instituto de Investigação e Inovação em Saúde, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade Do Porto, Rua Alfredo Allen, 208, 4200-135, Porto, Portugal; Corresponding author. i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal.Hybrid 3D constructs combining different structural components afford unique opportunities to engineer functional tissues. Creating functional microvascular networks within these constructs is crucial for promoting integration with host vessels and ensuring successful engraftment. Here, we present a hybrid 3D system in which poly (ethylene oxide terephthalate)/poly (butylene terephthalate) fibrous scaffolds are combined with pectin hydrogels to provide internal topography and guide the formation of microvascular beds. The sequence/method of seeding human endothelial cells (EC) and mesenchymal stromal cells (MSC) into the system had a significant impact on microvessel formation. Optimal results were obtained when EC were directly seeded onto the fibrous scaffold, followed by the addition of hydrogel-embedded MSC. This approach facilitated the development of highly oriented microvascular networks along the fibers. These networks were lumenized, supported by a basement membrane, and stabilized by pericyte-like cells, persisting for at least 28 days in vitro. Furthermore, culture under pro-angiogenic and osteoinductive conditions induced MSC osteogenic differentiation without impairing microvessel formation. Upon subcutaneous implantation in mice, the pre-vascularized constructs were infiltrated by host vessels, and human microvessels were still present after 2 weeks. Overall, the proposed hybrid 3D system, combined with an optimized cell-seeding protocol, offers an effective approach for directing the formation of robust and geometrically oriented microvessels, making it promising for tissue engineering applications.http://www.sciencedirect.com/science/article/pii/S2590006424003521Scaffold vascularizationVascularized tissueTherapeutic vascularizationCell contact guidanceRegenerative medicine |
| spellingShingle | Sara C. Neves Aureliana Sousa Diana S. Nascimento Iasmim D. Orge Sílvia A. Ferreira Carlos Mota Lorenzo Moroni Cristina C. Barrias Pedro L. Granja A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues Materials Today Bio Scaffold vascularization Vascularized tissue Therapeutic vascularization Cell contact guidance Regenerative medicine |
| title | A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues |
| title_full | A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues |
| title_fullStr | A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues |
| title_full_unstemmed | A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues |
| title_short | A hybrid construct with tailored 3D structure for directing pre-vascularization in engineered tissues |
| title_sort | hybrid construct with tailored 3d structure for directing pre vascularization in engineered tissues |
| topic | Scaffold vascularization Vascularized tissue Therapeutic vascularization Cell contact guidance Regenerative medicine |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424003521 |
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