Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study
Abstract Background Vitamin B plays a crucial role in pregnancy, influencing maternal and fetal health. However, the impact of Vitamin B metabolism alterations on pregnancy outcomes remains unclear. This study investigates the association between Vitamin B metabolism and adverse pregnancy outcomes (...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Pregnancy and Childbirth |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12884-025-07806-7 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849334150457196544 |
|---|---|
| author | Limin Peng Yan Gao Chengkun Yuan Hongying Kuang |
| author_facet | Limin Peng Yan Gao Chengkun Yuan Hongying Kuang |
| author_sort | Limin Peng |
| collection | DOAJ |
| description | Abstract Background Vitamin B plays a crucial role in pregnancy, influencing maternal and fetal health. However, the impact of Vitamin B metabolism alterations on pregnancy outcomes remains unclear. This study investigates the association between Vitamin B metabolism and adverse pregnancy outcomes (APO) using a retrospective approach. Methods A total of 1,086 pregnant women were included, categorized into normal and APO groups. Vitamin B markers during pregnancy, including red blood cell (RBC) and plasma levels of folate (5MTHF), Vitamin B2 (VB2), and Vitamin B6 (VB6), as well as methylmalonic acid (MMA) and homocysteine (HCY), were analyzed. LASSO regression was used for feature selection, followed by logistic regression to develop a predictive model. The model’s performance was evaluated using ROC curve, calibration curves, decision curve analysis, and external validation. Results Higher plasma-5MTHF, RBC-5MTHF, RBC-VB2, and RBC-VB6 levels were significantly associated with normal pregnancy outcomes, whereas elevated MMA and HCY levels correlated with an increased risk of APO (P < 0.05). In addition, maternal age, pregnancy type, pre-BMI, and passive smoking were identified as significant baseline factors. By integrating these baseline characteristics with Vitamin B-related biomarkers, the predictive model demonstrated excellent performance, with an AUC of 0.85 (95% CI: 0.818–0.883) in the training set and 0.807 (95% CI: 0.738–0.876) in the external validation set. Conclusion Alterations in Vitamin B metabolism are significantly associated with pregnancy outcomes. The developed predictive model provides a valuable tool for risk assessment in clinical practice. |
| format | Article |
| id | doaj-art-22a0f4aa61c842068c90dfd4b26168b2 |
| institution | Kabale University |
| issn | 1471-2393 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj-art-22a0f4aa61c842068c90dfd4b26168b22025-08-20T03:45:39ZengBMCBMC Pregnancy and Childbirth1471-23932025-07-0125111210.1186/s12884-025-07806-7Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective studyLimin Peng0Yan Gao1Chengkun Yuan2Hongying Kuang3Department of Obstetrics, The First Affiliated Hospital , Heilongjiang University of Chinese MedicineDepartment of Obstetrics, The First Affiliated Hospital , Heilongjiang University of Chinese MedicineDepartment of Obstetrics, The First Affiliated Hospital , Heilongjiang University of Chinese MedicineHeilongjiang University of Chinese MedicineAbstract Background Vitamin B plays a crucial role in pregnancy, influencing maternal and fetal health. However, the impact of Vitamin B metabolism alterations on pregnancy outcomes remains unclear. This study investigates the association between Vitamin B metabolism and adverse pregnancy outcomes (APO) using a retrospective approach. Methods A total of 1,086 pregnant women were included, categorized into normal and APO groups. Vitamin B markers during pregnancy, including red blood cell (RBC) and plasma levels of folate (5MTHF), Vitamin B2 (VB2), and Vitamin B6 (VB6), as well as methylmalonic acid (MMA) and homocysteine (HCY), were analyzed. LASSO regression was used for feature selection, followed by logistic regression to develop a predictive model. The model’s performance was evaluated using ROC curve, calibration curves, decision curve analysis, and external validation. Results Higher plasma-5MTHF, RBC-5MTHF, RBC-VB2, and RBC-VB6 levels were significantly associated with normal pregnancy outcomes, whereas elevated MMA and HCY levels correlated with an increased risk of APO (P < 0.05). In addition, maternal age, pregnancy type, pre-BMI, and passive smoking were identified as significant baseline factors. By integrating these baseline characteristics with Vitamin B-related biomarkers, the predictive model demonstrated excellent performance, with an AUC of 0.85 (95% CI: 0.818–0.883) in the training set and 0.807 (95% CI: 0.738–0.876) in the external validation set. Conclusion Alterations in Vitamin B metabolism are significantly associated with pregnancy outcomes. The developed predictive model provides a valuable tool for risk assessment in clinical practice.https://doi.org/10.1186/s12884-025-07806-7Vitamin B metabolismPregnancy outcomesPredictive model5MTHFExternal validation |
| spellingShingle | Limin Peng Yan Gao Chengkun Yuan Hongying Kuang Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study BMC Pregnancy and Childbirth Vitamin B metabolism Pregnancy outcomes Predictive model 5MTHF External validation |
| title | Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study |
| title_full | Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study |
| title_fullStr | Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study |
| title_full_unstemmed | Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study |
| title_short | Maternal red blood cell folate and vitamin B metabolism with pregnancy outcomes: a retrospective study |
| title_sort | maternal red blood cell folate and vitamin b metabolism with pregnancy outcomes a retrospective study |
| topic | Vitamin B metabolism Pregnancy outcomes Predictive model 5MTHF External validation |
| url | https://doi.org/10.1186/s12884-025-07806-7 |
| work_keys_str_mv | AT liminpeng maternalredbloodcellfolateandvitaminbmetabolismwithpregnancyoutcomesaretrospectivestudy AT yangao maternalredbloodcellfolateandvitaminbmetabolismwithpregnancyoutcomesaretrospectivestudy AT chengkunyuan maternalredbloodcellfolateandvitaminbmetabolismwithpregnancyoutcomesaretrospectivestudy AT hongyingkuang maternalredbloodcellfolateandvitaminbmetabolismwithpregnancyoutcomesaretrospectivestudy |