The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application

Impaired wound healing is a significant medical problem. Recently, cell-based therapy focused on stem cells has been developed to overcome the challenges of defective wound healing. In this study, we aimed to evaluate the effectiveness of adipose-derived stem cells (ASCs) in promoting wound healing,...

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Main Authors: Hyeonwoo Kim, Mi Ri Hyun, Sang Wha Kim
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2019/2745640
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author Hyeonwoo Kim
Mi Ri Hyun
Sang Wha Kim
author_facet Hyeonwoo Kim
Mi Ri Hyun
Sang Wha Kim
author_sort Hyeonwoo Kim
collection DOAJ
description Impaired wound healing is a significant medical problem. Recently, cell-based therapy focused on stem cells has been developed to overcome the challenges of defective wound healing. In this study, we aimed to evaluate the effectiveness of adipose-derived stem cells (ASCs) in promoting wound healing, using different techniques for administering them. Dorsal full-thickness skin defects (1×1 cm) were created in three groups of mice that received intravenous ASCs by intravenous injection, intramuscular injection, and topical application, respectively. Three control groups received saline in the same ways. Wound healing was assessed clinically, wounds were examined histologically, and GFP-labelled ASCs were detected with an IVIS imaging system. The results revealed that ASCs accelerated wound healing independent of their mode of administration. Histological examination showed that the ASCs accelerated reepithelialization, and IVIS analysis indicated that many ASCs were present in the wound area and disappeared after wound healing.
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spelling doaj-art-2291a69defd642b48b1196f0a67c09df2025-08-20T03:54:48ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/27456402745640The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of ApplicationHyeonwoo Kim0Mi Ri Hyun1Sang Wha Kim2Department of Plastic and Reconstructive Surgery, Seoul National University Hospital, Seoul, Republic of KoreaDepartment of Plastic and Reconstructive Surgery, Seoul National University Hospital, Seoul, Republic of KoreaDepartment of Plastic and Reconstructive Surgery, Seoul National University Hospital, Seoul, Republic of KoreaImpaired wound healing is a significant medical problem. Recently, cell-based therapy focused on stem cells has been developed to overcome the challenges of defective wound healing. In this study, we aimed to evaluate the effectiveness of adipose-derived stem cells (ASCs) in promoting wound healing, using different techniques for administering them. Dorsal full-thickness skin defects (1×1 cm) were created in three groups of mice that received intravenous ASCs by intravenous injection, intramuscular injection, and topical application, respectively. Three control groups received saline in the same ways. Wound healing was assessed clinically, wounds were examined histologically, and GFP-labelled ASCs were detected with an IVIS imaging system. The results revealed that ASCs accelerated wound healing independent of their mode of administration. Histological examination showed that the ASCs accelerated reepithelialization, and IVIS analysis indicated that many ASCs were present in the wound area and disappeared after wound healing.http://dx.doi.org/10.1155/2019/2745640
spellingShingle Hyeonwoo Kim
Mi Ri Hyun
Sang Wha Kim
The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
Stem Cells International
title The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
title_full The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
title_fullStr The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
title_full_unstemmed The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
title_short The Effect of Adipose-Derived Stem Cells on Wound Healing: Comparison of Methods of Application
title_sort effect of adipose derived stem cells on wound healing comparison of methods of application
url http://dx.doi.org/10.1155/2019/2745640
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