Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka
Surface water pollution from rapid urbanization, industrialization, inadequate sanitation, and excessive agrochemical use is a global crisis. In developing countries, water quality is one of the most urgent environmental issues. The presence of pathogenic extended spectrum β-lactamase (ESBL) produci...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025005997 |
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| author | Md. Sakib Hossain Ahmed Ishtiaque Amin Chowdhury Mohammad Rafiqul Islam Ripan Kirtunia Md. Foysal Abedin Mohammad Atique Ul Alam Shampa Sonia Binte Murshed Md Shadman Sakib Siam Alam M. Shahjahan Mondal Zahid Hayat Mahmud |
| author_facet | Md. Sakib Hossain Ahmed Ishtiaque Amin Chowdhury Mohammad Rafiqul Islam Ripan Kirtunia Md. Foysal Abedin Mohammad Atique Ul Alam Shampa Sonia Binte Murshed Md Shadman Sakib Siam Alam M. Shahjahan Mondal Zahid Hayat Mahmud |
| author_sort | Md. Sakib Hossain |
| collection | DOAJ |
| description | Surface water pollution from rapid urbanization, industrialization, inadequate sanitation, and excessive agrochemical use is a global crisis. In developing countries, water quality is one of the most urgent environmental issues. The presence of pathogenic extended spectrum β-lactamase (ESBL) producing E. coli in surface water poses a critical public health concern by increasing the risk of waterborne diseases and spreading multidrug-resistant (MDR) infections. This study addresses the severity of surface water pollution in urban Bangladesh, focusing on the molecular characterization of ESBL E. coli in surface waters from the Goranchatbari sub-catchment of Dhaka. Isolates of ESBL E. coli underwent analysis for the major ESBL and pathogenic genes, antibiotic susceptibility, biofilm formation, and genetic diversity. However, the existence of E. coli was confirmed in every sample. Among 266 isolates, 62 (23.31 %) were phenotypically positive for ESBL, with 58 (93.55 %) carrying at least one of the four ESBL genes: CTX-M, TEM, SHV, and OXA. CTX-M was the most prevalent, found in 55 (88.71 %) isolates. Regarding pathogenicity, 25 (40.32 %) isolates were enteric pathogens, including 24 ETEC and a single EIEC. Four non-diarrheagenic isolates were extraintestinal pathogenic E. coli (ExPEC), capable of causing diseases beyond enteric infections. All ESBL isolates were MDR, with high resistance to β-lactam antibiotics, and capable of forming biofilms at 25 °C and 37 °C. ERIC-PCR analysis grouped the isolates into 14 distinct clusters at a 75 % similarity matrix. These water sources critically threaten public health by contaminating nearby freshwater sources with ESBL-producing pathogenic isolates, leading to various hard-to-treat waterborne diseases and risking aquatic biota by deteriorating water quality. Immediate public awareness, proper water treatment, and precise environmental management are needed before using this water for any purpose. |
| format | Article |
| id | doaj-art-228fcf6bd9bc4933b7cbe5b33c0523b0 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-228fcf6bd9bc4933b7cbe5b33c0523b02025-01-30T05:14:39ZengElsevierHeliyon2405-84402025-02-01113e42219Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, DhakaMd. Sakib Hossain0Ahmed Ishtiaque Amin Chowdhury1Mohammad Rafiqul Islam2Ripan Kirtunia3Md. Foysal Abedin4Mohammad Atique Ul Alam5 Shampa6Sonia Binte Murshed7Md Shadman Sakib8Siam Alam9M. Shahjahan Mondal10Zahid Hayat Mahmud11Laboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, 1212, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, Bangladesh; Corresponding author. Institute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Polashi Road, Dhaka, 1000, Bangladesh.Laboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, 1212, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, BangladeshLaboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, 1212, BangladeshLaboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, 1212, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, Bangladesh; Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, 24061, USAInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, BangladeshInstitute of Water and Flood Management (IWFM), Bangladesh University of Engineering and Technology (BUET), Dhaka, 1000, BangladeshLaboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka, 1212, Bangladesh; Corresponding author. Laboratory of Environmental Health, Health Systems and Population Studies Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), 68, Shaheed Tajuddin Ahmed Sarani, Mohakhali, Dhaka, 1212, Bangladesh.Surface water pollution from rapid urbanization, industrialization, inadequate sanitation, and excessive agrochemical use is a global crisis. In developing countries, water quality is one of the most urgent environmental issues. The presence of pathogenic extended spectrum β-lactamase (ESBL) producing E. coli in surface water poses a critical public health concern by increasing the risk of waterborne diseases and spreading multidrug-resistant (MDR) infections. This study addresses the severity of surface water pollution in urban Bangladesh, focusing on the molecular characterization of ESBL E. coli in surface waters from the Goranchatbari sub-catchment of Dhaka. Isolates of ESBL E. coli underwent analysis for the major ESBL and pathogenic genes, antibiotic susceptibility, biofilm formation, and genetic diversity. However, the existence of E. coli was confirmed in every sample. Among 266 isolates, 62 (23.31 %) were phenotypically positive for ESBL, with 58 (93.55 %) carrying at least one of the four ESBL genes: CTX-M, TEM, SHV, and OXA. CTX-M was the most prevalent, found in 55 (88.71 %) isolates. Regarding pathogenicity, 25 (40.32 %) isolates were enteric pathogens, including 24 ETEC and a single EIEC. Four non-diarrheagenic isolates were extraintestinal pathogenic E. coli (ExPEC), capable of causing diseases beyond enteric infections. All ESBL isolates were MDR, with high resistance to β-lactam antibiotics, and capable of forming biofilms at 25 °C and 37 °C. ERIC-PCR analysis grouped the isolates into 14 distinct clusters at a 75 % similarity matrix. These water sources critically threaten public health by contaminating nearby freshwater sources with ESBL-producing pathogenic isolates, leading to various hard-to-treat waterborne diseases and risking aquatic biota by deteriorating water quality. Immediate public awareness, proper water treatment, and precise environmental management are needed before using this water for any purpose.http://www.sciencedirect.com/science/article/pii/S2405844025005997Surface waterWaterborne diseasesESBL E. coliMultidrug resistantPathogenicityPublic health |
| spellingShingle | Md. Sakib Hossain Ahmed Ishtiaque Amin Chowdhury Mohammad Rafiqul Islam Ripan Kirtunia Md. Foysal Abedin Mohammad Atique Ul Alam Shampa Sonia Binte Murshed Md Shadman Sakib Siam Alam M. Shahjahan Mondal Zahid Hayat Mahmud Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka Heliyon Surface water Waterborne diseases ESBL E. coli Multidrug resistant Pathogenicity Public health |
| title | Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka |
| title_full | Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka |
| title_fullStr | Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka |
| title_full_unstemmed | Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka |
| title_short | Multidrug-resistant ESBL E. coli in urban surface waters and public health implications: A Case Study from Goranchatbari, Dhaka |
| title_sort | multidrug resistant esbl e coli in urban surface waters and public health implications a case study from goranchatbari dhaka |
| topic | Surface water Waterborne diseases ESBL E. coli Multidrug resistant Pathogenicity Public health |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025005997 |
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