Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia

Investigating the gut microbiome during host development is essential for understanding its influence on host health and fitness. While host body condition is a crucial fitness-related trait and a strong predictor of viability in numerous animal species, its relationship with gut bacteria remains un...

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Main Authors: Longru Jin, Pai Zhang, Keping Sun, Haitao Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1595357/full
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author Longru Jin
Longru Jin
Longru Jin
Longru Jin
Pai Zhang
Pai Zhang
Pai Zhang
Pai Zhang
Keping Sun
Keping Sun
Keping Sun
Keping Sun
Haitao Wang
Haitao Wang
Haitao Wang
Haitao Wang
author_facet Longru Jin
Longru Jin
Longru Jin
Longru Jin
Pai Zhang
Pai Zhang
Pai Zhang
Pai Zhang
Keping Sun
Keping Sun
Keping Sun
Keping Sun
Haitao Wang
Haitao Wang
Haitao Wang
Haitao Wang
author_sort Longru Jin
collection DOAJ
description Investigating the gut microbiome during host development is essential for understanding its influence on host health and fitness. While host body condition is a crucial fitness-related trait and a strong predictor of viability in numerous animal species, its relationship with gut bacteria remains underexplored, particularly in non-model organisms. This study examines the gut microbiome of the altricial wild bird species, yellow-rumped flycatchers (Ficedula zanthopygia), by analyzing nestling feces through 16S rRNA sequencing at four developmental stages: Day 3, Day 6, Day 9 and Day 12 post-hatching. We explored the temporal dynamics of the gut microbiome and its correlation with body condition, a key indicator of fitness. Our results demonstrate signinficant shifts in microbial community composition and diversity throughout development. Notably, Day 3 nestlings displayed lower alpha diversity compared to later stages, while microbial diversity stabilized from Days 6 to 12. Both the age of the nestlings and the environmental conditions of the nest box significantly shaped the gut microbial community structure. A contemporaneous relationship was observed, where the scaled-mass index (SMI) at Day 6 positively correlating with microbial diversity at that time. Additionally, a time-lagged effect emerged, linking SMI at Day 9 to microbial diversity at Day 6. These findings highlight the vital role of the gut microbiome in the development of nestlings, particularly emphasizing Day 6 as a critical period due to its stable microbial diversity and association with SMI. This study underscores the influence of gut bacteria on host fitness in developing birds.
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spelling doaj-art-228b71b6ad3a49429eadf2c8d38fb3bf2025-08-20T02:00:58ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15953571595357Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygiaLongru Jin0Longru Jin1Longru Jin2Longru Jin3Pai Zhang4Pai Zhang5Pai Zhang6Pai Zhang7Keping Sun8Keping Sun9Keping Sun10Keping Sun11Haitao Wang12Haitao Wang13Haitao Wang14Haitao Wang15Jilin Engineering Laboratory for Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun, ChinaJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, ChinaJilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Northeast Normal University, Changchun, ChinaKey Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaJilin Engineering Laboratory for Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun, ChinaJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, ChinaJilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Northeast Normal University, Changchun, ChinaKey Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaJilin Engineering Laboratory for Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun, ChinaJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, ChinaJilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Northeast Normal University, Changchun, ChinaKey Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaJilin Engineering Laboratory for Avian Ecology and Conservation Genetics, Northeast Normal University, Changchun, ChinaJilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, Northeast Normal University, Changchun, ChinaJilin Provincial International Cooperation Key Laboratory for Biological Control of Agricultural Pests, Northeast Normal University, Changchun, ChinaKey Laboratory of Vegetation Ecology, Ministry of Education, Changchun, ChinaInvestigating the gut microbiome during host development is essential for understanding its influence on host health and fitness. While host body condition is a crucial fitness-related trait and a strong predictor of viability in numerous animal species, its relationship with gut bacteria remains underexplored, particularly in non-model organisms. This study examines the gut microbiome of the altricial wild bird species, yellow-rumped flycatchers (Ficedula zanthopygia), by analyzing nestling feces through 16S rRNA sequencing at four developmental stages: Day 3, Day 6, Day 9 and Day 12 post-hatching. We explored the temporal dynamics of the gut microbiome and its correlation with body condition, a key indicator of fitness. Our results demonstrate signinficant shifts in microbial community composition and diversity throughout development. Notably, Day 3 nestlings displayed lower alpha diversity compared to later stages, while microbial diversity stabilized from Days 6 to 12. Both the age of the nestlings and the environmental conditions of the nest box significantly shaped the gut microbial community structure. A contemporaneous relationship was observed, where the scaled-mass index (SMI) at Day 6 positively correlating with microbial diversity at that time. Additionally, a time-lagged effect emerged, linking SMI at Day 9 to microbial diversity at Day 6. These findings highlight the vital role of the gut microbiome in the development of nestlings, particularly emphasizing Day 6 as a critical period due to its stable microbial diversity and association with SMI. This study underscores the influence of gut bacteria on host fitness in developing birds.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1595357/fullgut microbiomeyellow-rumped flycatchermicrobial diversitynestlingtemporal dynamicsSMI
spellingShingle Longru Jin
Longru Jin
Longru Jin
Longru Jin
Pai Zhang
Pai Zhang
Pai Zhang
Pai Zhang
Keping Sun
Keping Sun
Keping Sun
Keping Sun
Haitao Wang
Haitao Wang
Haitao Wang
Haitao Wang
Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
Frontiers in Microbiology
gut microbiome
yellow-rumped flycatcher
microbial diversity
nestling
temporal dynamics
SMI
title Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
title_full Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
title_fullStr Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
title_full_unstemmed Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
title_short Gut microbiota dynamics and their impact on body condition in nestlings of the yellow-rumped flycatchers, Ficedula zanthopygia
title_sort gut microbiota dynamics and their impact on body condition in nestlings of the yellow rumped flycatchers ficedula zanthopygia
topic gut microbiome
yellow-rumped flycatcher
microbial diversity
nestling
temporal dynamics
SMI
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1595357/full
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