POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE)
Species identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwithth...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/52 |
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| author | O. V. Vaulin Yu. M. Novikov |
| author_facet | O. V. Vaulin Yu. M. Novikov |
| author_sort | O. V. Vaulin |
| collection | DOAJ |
| description | Species identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwiththose from a DNA database. VariabilityofCOI nucleotide sequences (measures H andπ) ofthe crypticspecies A andB Anopheles messeae andAn. beklemishevi was investigatedandquantitativelyestimated. It was foundthat the COI variabilitydidnot allow discrimination ofthe crypticspecies A andB Anopheles messeae. Neither didit show anygeographicregularityfor these species. It is concludedthat the COI polymorphism ofthe A andB Anopheles messeae species is common for them, as well as the chromosomal inversion polymorphism. It arose long before their divergence. The diagnosticimportance ofinterspecificITS2 variabilityis confirmed. |
| format | Article |
| id | doaj-art-2286033366c141d7a43f63e80417d8b4 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-2286033366c141d7a43f63e80417d8b42025-02-01T09:57:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-0116235836836POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE)O. V. Vaulin0Yu. M. Novikov1Institute of Cytology and Genetics, SB RAS, Novosibirsk, RussiaTomsk State University, Tomsk, RussiaSpecies identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwiththose from a DNA database. VariabilityofCOI nucleotide sequences (measures H andπ) ofthe crypticspecies A andB Anopheles messeae andAn. beklemishevi was investigatedandquantitativelyestimated. It was foundthat the COI variabilitydidnot allow discrimination ofthe crypticspecies A andB Anopheles messeae. Neither didit show anygeographicregularityfor these species. It is concludedthat the COI polymorphism ofthe A andB Anopheles messeae species is common for them, as well as the chromosomal inversion polymorphism. It arose long before their divergence. The diagnosticimportance ofinterspecificITS2 variabilityis confirmed.https://vavilov.elpub.ru/jour/article/view/52anopheles maculipennismesseaebeklemishevicrypyptic speciesits2coivariabilitypolymorphism |
| spellingShingle | O. V. Vaulin Yu. M. Novikov POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) Вавиловский журнал генетики и селекции anopheles maculipennis messeae beklemishevi crypyptic species its2 coi variability polymorphism |
| title | POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) |
| title_full | POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) |
| title_fullStr | POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) |
| title_full_unstemmed | POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) |
| title_short | POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE) |
| title_sort | polymorphism and interspecific variability of cytochrome oxydase subunit i coi gene nucleotide sequence in sibling species of a and b anopheles messeae and an beklemishevi diptera culicidae |
| topic | anopheles maculipennis messeae beklemishevi crypyptic species its2 coi variability polymorphism |
| url | https://vavilov.elpub.ru/jour/article/view/52 |
| work_keys_str_mv | AT ovvaulin polymorphismandinterspecificvariabilityofcytochromeoxydasesubuniticoigenenucleotidesequenceinsiblingspeciesofaandbanophelesmesseaeandanbeklemishevidipteraculicidae AT yumnovikov polymorphismandinterspecificvariabilityofcytochromeoxydasesubuniticoigenenucleotidesequenceinsiblingspeciesofaandbanophelesmesseaeandanbeklemishevidipteraculicidae |