Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response
Thymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidin...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/306754 |
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| author | A. Calascibetta Flavia Contino S. Feo G. Gulotta M. Cajozzo A. Antona G. Sanguedolce R. Sanguedolce |
| author_facet | A. Calascibetta Flavia Contino S. Feo G. Gulotta M. Cajozzo A. Antona G. Sanguedolce R. Sanguedolce |
| author_sort | A. Calascibetta |
| collection | DOAJ |
| description | Thymidylate synthase (TS) catalyzes methylation
of dUMP to dTMP and it is the target for the
5-Fluorouracil (5-FU) activity. Barbour et al.
showed that variant structural forms of TS in tumour
cell lines confer resistance to fluoropyrimidines.
We planned to perform the whole TS gene structure by
means of sequencing techniques in human colorectal
cancer (CRC) samples to try to identify the presence
of any possible TS variant form that could be
responsible of fluoropyrimidines drug resistance and
of the worse prognosis. We performed the TS-DNA gene
sequence in 68 CRC from patients of A, B, and C
Dukes' stages and different histological grade,
but we did not find any mutation in the TS-DNA
structure. In the future we intend to widen the TS
structure analysis to the metastatic CRCs,
because due to their higher genomic
instability, they could present a TS variant form
responsible of the fluoropyrimidines drug resistance
and the worse prognosis. |
| format | Article |
| id | doaj-art-2284fe57696243859265ca02b9fa81be |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-2284fe57696243859265ca02b9fa81be2025-02-03T07:24:34ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/306754306754Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug ResponseA. Calascibetta0Flavia Contino1S. Feo2G. Gulotta3M. Cajozzo4A. Antona5G. Sanguedolce6R. Sanguedolce7Dipartimento di Scienze Farmacologiche “Pietro Benigno,” Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Oncologia Sperimentale e Applicazioni Cliniche, Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Oncologia Sperimentale e Applicazioni Cliniche, Università degli Studi di Palermo, 90100 Palermo, ItalyChirurgia Generale e d'Urgenza, Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Discipline Chirurgiche ed Oncologiche, Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Scienze Farmacologiche “Pietro Benigno,” Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Scienze Farmacologiche “Pietro Benigno,” Università degli Studi di Palermo, 90100 Palermo, ItalyDipartimento di Scienze Farmacologiche “Pietro Benigno,” Università degli Studi di Palermo, 90100 Palermo, ItalyThymidylate synthase (TS) catalyzes methylation of dUMP to dTMP and it is the target for the 5-Fluorouracil (5-FU) activity. Barbour et al. showed that variant structural forms of TS in tumour cell lines confer resistance to fluoropyrimidines. We planned to perform the whole TS gene structure by means of sequencing techniques in human colorectal cancer (CRC) samples to try to identify the presence of any possible TS variant form that could be responsible of fluoropyrimidines drug resistance and of the worse prognosis. We performed the TS-DNA gene sequence in 68 CRC from patients of A, B, and C Dukes' stages and different histological grade, but we did not find any mutation in the TS-DNA structure. In the future we intend to widen the TS structure analysis to the metastatic CRCs, because due to their higher genomic instability, they could present a TS variant form responsible of the fluoropyrimidines drug resistance and the worse prognosis.http://dx.doi.org/10.4061/2010/306754 |
| spellingShingle | A. Calascibetta Flavia Contino S. Feo G. Gulotta M. Cajozzo A. Antona G. Sanguedolce R. Sanguedolce Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response Journal of Nucleic Acids |
| title | Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response |
| title_full | Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response |
| title_fullStr | Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response |
| title_full_unstemmed | Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response |
| title_short | Analysis of the Thymidylate Synthase Gene Structure in Colorectal Cancer Patients and Its Possible Relation with the 5-Fluorouracil Drug Response |
| title_sort | analysis of the thymidylate synthase gene structure in colorectal cancer patients and its possible relation with the 5 fluorouracil drug response |
| url | http://dx.doi.org/10.4061/2010/306754 |
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