Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models
Background. We used high-fat (HF), high-fructose (HFr), and combination diets to create a dietary animal model of nonalcoholic fatty liver disease (NAFLD). Comparison of both clinical phenotypes has not been well defined. The purpose of this study was to compare histologic and metabolic characterist...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/306326 |
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| _version_ | 1849307271244283904 |
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| author | Jai Sun Lee Dae Won Jun Eun Kyung Kim Hye Joon Jeon Ho Hyun Nam Waqar Khalid Saeed |
| author_facet | Jai Sun Lee Dae Won Jun Eun Kyung Kim Hye Joon Jeon Ho Hyun Nam Waqar Khalid Saeed |
| author_sort | Jai Sun Lee |
| collection | DOAJ |
| description | Background. We used high-fat (HF), high-fructose (HFr), and combination diets to create a dietary animal model of nonalcoholic fatty liver disease (NAFLD). Comparison of both clinical phenotypes has not been well defined. The purpose of this study was to compare histologic and metabolic characteristics between diets in an animal model of NAFLD.
Methods. NAFLD was induced in rats by feeding them HF, HFr, and combination (HF + HFr) diets for 20 weeks. The degree of intrahepatic fat accumulation, inflammation, and oxidative stress was evaluated. Metabolic derangements were assessed by the oral glucose tolerance test and the intrahepatic insulin signal pathway. Results. Body weight gain and intrahepatic fat accumulation were more prominent in the HF feeding group than in the HFr group. The expressions of NOX-4 and TLR-4 were higher in the HF and HFr combination groups than in the HF-only group. Other intrahepatic inflammatory markers, MCP-1, TNF-α, and endoplasmic reticulum stress markers, were the highest in the HF + HFr combination group. Although intrahepatic fat deposition was less prominent in the HFr diet model, intrahepatic inflammation was noted. Conclusions. Intrahepatic inflammation and metabolic derangements were more prominent in the HF and HFr combination model than in the HF monodiet model. |
| format | Article |
| id | doaj-art-22849bfc231b4100bd00bcdd85b7115c |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-22849bfc231b4100bd00bcdd85b7115c2025-08-20T03:54:48ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/306326306326Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal ModelsJai Sun Lee0Dae Won Jun1Eun Kyung Kim2Hye Joon Jeon3Ho Hyun Nam4Waqar Khalid Saeed5Department Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of KoreaDepartment Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of KoreaDepartment of Pathology, College of Medicine, Eulji University, Seoul, Republic of KoreaDepartment of Internal Medicine, School of Medicine, Hanyang University, Seoul, Republic of KoreaDepartment Translational Medicine, Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, Republic of KoreaDepartment of Internal Medicine, School of Medicine, Hanyang University, Seoul, Republic of KoreaBackground. We used high-fat (HF), high-fructose (HFr), and combination diets to create a dietary animal model of nonalcoholic fatty liver disease (NAFLD). Comparison of both clinical phenotypes has not been well defined. The purpose of this study was to compare histologic and metabolic characteristics between diets in an animal model of NAFLD. Methods. NAFLD was induced in rats by feeding them HF, HFr, and combination (HF + HFr) diets for 20 weeks. The degree of intrahepatic fat accumulation, inflammation, and oxidative stress was evaluated. Metabolic derangements were assessed by the oral glucose tolerance test and the intrahepatic insulin signal pathway. Results. Body weight gain and intrahepatic fat accumulation were more prominent in the HF feeding group than in the HFr group. The expressions of NOX-4 and TLR-4 were higher in the HF and HFr combination groups than in the HF-only group. Other intrahepatic inflammatory markers, MCP-1, TNF-α, and endoplasmic reticulum stress markers, were the highest in the HF + HFr combination group. Although intrahepatic fat deposition was less prominent in the HFr diet model, intrahepatic inflammation was noted. Conclusions. Intrahepatic inflammation and metabolic derangements were more prominent in the HF and HFr combination model than in the HF monodiet model.http://dx.doi.org/10.1155/2015/306326 |
| spellingShingle | Jai Sun Lee Dae Won Jun Eun Kyung Kim Hye Joon Jeon Ho Hyun Nam Waqar Khalid Saeed Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models The Scientific World Journal |
| title | Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models |
| title_full | Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models |
| title_fullStr | Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models |
| title_full_unstemmed | Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models |
| title_short | Histologic and Metabolic Derangement in High-Fat, High-Fructose, and Combination Diet Animal Models |
| title_sort | histologic and metabolic derangement in high fat high fructose and combination diet animal models |
| url | http://dx.doi.org/10.1155/2015/306326 |
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