Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand
To establish a new breeding program, genetic diversity of five domesticated populations and two commercial stocks of Asian seabass (Lates calcarifer) were examined. Moderate levels of genetic diversity based on microsatellite polymorphism were found. The number of alleles per locus (NA) was 3.429–11...
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KeAi Communications Co., Ltd.
2025-07-01
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| Series: | Aquaculture and Fisheries |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X24000583 |
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| author | Sirikan Prasertlux Atra Chaimongkol Jutaporn Chaimongkol Sirithorn Janpoom Sureerat Tang Puttawan Rongmung Wanwipa Ittarat Parichart Ninwichian Putth Songsangjinda Takashi Sakamoto Bavornlak Khamnamtong Panya Sae-Lim Sirawut Klinbunga |
| author_facet | Sirikan Prasertlux Atra Chaimongkol Jutaporn Chaimongkol Sirithorn Janpoom Sureerat Tang Puttawan Rongmung Wanwipa Ittarat Parichart Ninwichian Putth Songsangjinda Takashi Sakamoto Bavornlak Khamnamtong Panya Sae-Lim Sirawut Klinbunga |
| author_sort | Sirikan Prasertlux |
| collection | DOAJ |
| description | To establish a new breeding program, genetic diversity of five domesticated populations and two commercial stocks of Asian seabass (Lates calcarifer) were examined. Moderate levels of genetic diversity based on microsatellite polymorphism were found. The number of alleles per locus (NA) was 3.429–11.571. Observed (Ho) and expected (He) heterozygosities were 0.416–0.772 and 0.423–0.805. FST and genetic heterogeneity analysis revealed significant genetic differences of most pairwise comparisons (P < 0.001). Genetic distance between populations was 0.000–0.703. Phylogenetic analysis divided examined populations to two genetic stocks; A: DOF-Chachoengsao 2, DOF-Songkhla, and DOF-Trang and B: Commercial Farm 1 (Phuket) and Commercial Farm 2 (Chachengsao), DOF-Chachoengsao 1 and DOF-Krabi. The base population (G0, 51 families) was established from crosses between these founder populations. Heritability and estimated breeding values (EBVs) for growth in G0 families were evaluated at 519 day post hatch (dph, mean body weight = 616.64 ± 266.80 g, N = 1655). The heritability (h2) for growth of L. calcarifer in this study was 0.386 ± 0.020. The additive genetic coefficient of variation was 28% indicating high potential of genetic improvement for growth in this population. The distribution of EBVs showed high variation both between and within families. Fish exhibiting high EBVs (SATREPS-DOF-G0-hEBVs) clustered with a phylogenetic clade A while the SATREPS-DOF-G0-lEBVs group was phylogenetically allocated to clade B. Genetic selection with +1SD criteria is expected to achieve genetic gain of 28.5% per generation. The information on genetic diversity and EBVs estimates allows efficient implementation of our ongoing breeding program of L. calcarifer. |
| format | Article |
| id | doaj-art-227f6cb52ebc4868982ad021e3d3a8cc |
| institution | DOAJ |
| issn | 2468-550X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | KeAi Communications Co., Ltd. |
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| series | Aquaculture and Fisheries |
| spelling | doaj-art-227f6cb52ebc4868982ad021e3d3a8cc2025-08-20T03:13:14ZengKeAi Communications Co., Ltd.Aquaculture and Fisheries2468-550X2025-07-0110457658510.1016/j.aaf.2024.04.006Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from ThailandSirikan Prasertlux0Atra Chaimongkol1Jutaporn Chaimongkol2Sirithorn Janpoom3Sureerat Tang4Puttawan Rongmung5Wanwipa Ittarat6Parichart Ninwichian7Putth Songsangjinda8Takashi Sakamoto9Bavornlak Khamnamtong10Panya Sae-Lim11Sirawut Klinbunga12Aquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandCoastal Aquaculture Technology and Innovation Research and Development Center, Department of Fisheries, Ministry of Agriculture and Cooperative, Songkhla, 80170, ThailandCoastal Aquaculture Technology and Innovation Research and Development Center, Department of Fisheries, Ministry of Agriculture and Cooperative, Songkhla, 80170, ThailandAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandFaculty of Innovative Agriculture and Fishery Establishment Project, Prince of Songkla University, Surat Thani Campus, Surat Thani, 84000, ThailandDepartment of Fisheries, Ministry of Agriculture and Cooperative, Chatuchak, Bangkok, 10900, ThailandDepartment of Aquatic Biosciences, Tokyo University of Marine Science and Technology, Tokyo, 108-8477, JapanAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, Thailand; Corresponding author.Faculty of Veterinary Medicine, Rajamangala University of Technology Srivijaya, Songkhla, 90000, ThailandAquatic Molecular Genetics and Biotechnology Research Team, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Khlong Nueng, Khlong Luang, Pathum Thani, 12120, ThailandTo establish a new breeding program, genetic diversity of five domesticated populations and two commercial stocks of Asian seabass (Lates calcarifer) were examined. Moderate levels of genetic diversity based on microsatellite polymorphism were found. The number of alleles per locus (NA) was 3.429–11.571. Observed (Ho) and expected (He) heterozygosities were 0.416–0.772 and 0.423–0.805. FST and genetic heterogeneity analysis revealed significant genetic differences of most pairwise comparisons (P < 0.001). Genetic distance between populations was 0.000–0.703. Phylogenetic analysis divided examined populations to two genetic stocks; A: DOF-Chachoengsao 2, DOF-Songkhla, and DOF-Trang and B: Commercial Farm 1 (Phuket) and Commercial Farm 2 (Chachengsao), DOF-Chachoengsao 1 and DOF-Krabi. The base population (G0, 51 families) was established from crosses between these founder populations. Heritability and estimated breeding values (EBVs) for growth in G0 families were evaluated at 519 day post hatch (dph, mean body weight = 616.64 ± 266.80 g, N = 1655). The heritability (h2) for growth of L. calcarifer in this study was 0.386 ± 0.020. The additive genetic coefficient of variation was 28% indicating high potential of genetic improvement for growth in this population. The distribution of EBVs showed high variation both between and within families. Fish exhibiting high EBVs (SATREPS-DOF-G0-hEBVs) clustered with a phylogenetic clade A while the SATREPS-DOF-G0-lEBVs group was phylogenetically allocated to clade B. Genetic selection with +1SD criteria is expected to achieve genetic gain of 28.5% per generation. The information on genetic diversity and EBVs estimates allows efficient implementation of our ongoing breeding program of L. calcarifer.http://www.sciencedirect.com/science/article/pii/S2468550X24000583Genetic diversityAsian seabassLates calcariferBreedingEBVsGrowth |
| spellingShingle | Sirikan Prasertlux Atra Chaimongkol Jutaporn Chaimongkol Sirithorn Janpoom Sureerat Tang Puttawan Rongmung Wanwipa Ittarat Parichart Ninwichian Putth Songsangjinda Takashi Sakamoto Bavornlak Khamnamtong Panya Sae-Lim Sirawut Klinbunga Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand Aquaculture and Fisheries Genetic diversity Asian seabass Lates calcarifer Breeding EBVs Growth |
| title | Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand |
| title_full | Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand |
| title_fullStr | Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand |
| title_full_unstemmed | Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand |
| title_short | Genetic diversity, heritability, and estimated breeding values for growth of domesticated Asian seabass Lates calcarifer from Thailand |
| title_sort | genetic diversity heritability and estimated breeding values for growth of domesticated asian seabass lates calcarifer from thailand |
| topic | Genetic diversity Asian seabass Lates calcarifer Breeding EBVs Growth |
| url | http://www.sciencedirect.com/science/article/pii/S2468550X24000583 |
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