Analysis of the Differences Among <i>Camellia oleifera</i> Grafting Combinations in Its Healing Process

Grafting serves as a crucial propagation technique for superior <i>Camellia oleifera</i> varieties, where rootstock–scion compatibility significantly determines survival and growth performance. To systematically evaluate grafting compatibility in this economically important woody oil cro...

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Main Authors: Zhilong He, Ying Zhang, Chengfeng Xun, Zhen Zhang, Yushen Ma, Xin Wei, Zhentao Wan, Rui Wang
Format: Article
Language:English
Published: MDPI AG 2025-08-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/15/2432
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Summary:Grafting serves as a crucial propagation technique for superior <i>Camellia oleifera</i> varieties, where rootstock–scion compatibility significantly determines survival and growth performance. To systematically evaluate grafting compatibility in this economically important woody oil crop, we examined 15 rootstock–scion combinations using ‘Xianglin 210’ as the scion, assessing growth traits and conducting physiological assays (enzymatic activities of SOD and POD and levels of ROS and IAA) at multiple timepoints (0–32 days post-grafting). The results demonstrated that Comb. 4 (Xianglin 27 rootstock) exhibited superior compatibility, characterized by systemic antioxidant activation (peaking at 4–8 DPG), rapid auxin accumulation (4 DPG), and efficient sugar allocation. Transcriptome sequencing and WGCNA analysis identified 3781 differentially expressed genes, with notable enrichment in stress response pathways (Hsp70, DnaJ) and auxin biosynthesis (YUCCA), while also revealing key hub genes (FKBP19) associated with graft-healing efficiency. These findings establish that successful grafting in <i>C. oleifera</i> depends on coordinated rapid redox regulation, auxin-mediated cell proliferation, and metabolic reprogramming, with Comb. 4 emerging as the optimal rootstock choice. The identified molecular markers not only advance our understanding of grafting mechanisms in woody plants but also provide valuable targets for future breeding programs aimed at improving grafting success rates in this important oil crop.
ISSN:2223-7747