Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study
BackgroundSepsis is a life-threatening disease with challenges in clinical management due to delayed diagnosis and immunosuppression. Lymphopenia is a key prognostic indicator in sepsis. microRNAs (miRNAs) are recognized as key immune modulators affecting all stages of inflammation. In this study, w...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1608576/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849682930804195328 |
|---|---|
| author | Sha Cheng Jiaojiao Wang Pengfei Wang Xianli Long Huiling Liu Xueyin Li Xiaowei Zhang Xuemei Zhao Hang Sun Chuanxin Wu |
| author_facet | Sha Cheng Jiaojiao Wang Pengfei Wang Xianli Long Huiling Liu Xueyin Li Xiaowei Zhang Xuemei Zhao Hang Sun Chuanxin Wu |
| author_sort | Sha Cheng |
| collection | DOAJ |
| description | BackgroundSepsis is a life-threatening disease with challenges in clinical management due to delayed diagnosis and immunosuppression. Lymphopenia is a key prognostic indicator in sepsis. microRNAs (miRNAs) are recognized as key immune modulators affecting all stages of inflammation. In this study, we investigated the expression of miR-146b-5p and lymphocyte subsets in sepsis patients and analyzed their correlation with the aim of establishing their combined predictive value for clinical outcomes and advancing personalized treatment strategies.MethodsFrom January 2020 to July 2024, we enrolled 191 patients diagnosed with sepsis at the ICU of the Second Affiliated Hospital of Chongqing Medical University and collected basic clinical data. These patients were categorized into two groups: nonsurvivors (n = 117) and survivors (n = 74). Correlation analysis was employed to analyze the correlation between miR-146b-5p and lymphocyte subsets with disease severity. Binary logistic regression and Cox regression analyses were employed to identify independent risk factors influencing the prognosis of sepsis. The predictive value of miR-146b-5p and lymphocyte subsets for sepsis prognosis was assessed using receiver operating characteristic (ROC) curves.ResultsmiR-146b-5p expression was significantly lower in sepsis patients compared to HD group, with levels in the nonsurvivor group being lower than those in the survivor group. Survival curves for miR-146b-5p indicated that lower levels of miR-146b-5p (<0.272) were associated with a higher mortality rate (HR 3.063). The absolute counts of lymphocytes (Lym), CD3+ T cells, CD4+ T cells, and CD8+ T cells were significantly lower in the sepsis group compared to the HD group. Testing lymphocyte counts at different time points revealed that absolute counts of CD3+ T cells, CD4+ T cells, and CD8+ T cells were consistently lower in the sepsis group across all time intervals. miR-146b-5p levels were predictive of patient prognosis, with the combination of miR-146b-5p and APACHE II scores yielding the highest AUC.ConclusionThe early-stage sepsis-associated downregulation of miR-146b-5p serves as a promising biomarker for severity stratification and prognostic evaluation. The combination of miR-146b-5p with APACHE II scores enhances diagnostic accuracy. Additionally, dynamic monitoring of lymphocyte subsets may facilitate the evaluation of immune status and guide personalized treatment strategies. |
| format | Article |
| id | doaj-art-227791ca60cb49e098fe32bdf585f383 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-227791ca60cb49e098fe32bdf585f3832025-08-20T03:24:02ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.16085761608576Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center studySha Cheng0Jiaojiao Wang1Pengfei Wang2Xianli Long3Huiling Liu4Xueyin Li5Xiaowei Zhang6Xuemei Zhao7Hang Sun8Chuanxin Wu9Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Intensive Care Unit, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaKey Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Hepatobiliary Surgery, The Second Affiliated Hospital, Chongqing Medical University, Chongqing, ChinaBackgroundSepsis is a life-threatening disease with challenges in clinical management due to delayed diagnosis and immunosuppression. Lymphopenia is a key prognostic indicator in sepsis. microRNAs (miRNAs) are recognized as key immune modulators affecting all stages of inflammation. In this study, we investigated the expression of miR-146b-5p and lymphocyte subsets in sepsis patients and analyzed their correlation with the aim of establishing their combined predictive value for clinical outcomes and advancing personalized treatment strategies.MethodsFrom January 2020 to July 2024, we enrolled 191 patients diagnosed with sepsis at the ICU of the Second Affiliated Hospital of Chongqing Medical University and collected basic clinical data. These patients were categorized into two groups: nonsurvivors (n = 117) and survivors (n = 74). Correlation analysis was employed to analyze the correlation between miR-146b-5p and lymphocyte subsets with disease severity. Binary logistic regression and Cox regression analyses were employed to identify independent risk factors influencing the prognosis of sepsis. The predictive value of miR-146b-5p and lymphocyte subsets for sepsis prognosis was assessed using receiver operating characteristic (ROC) curves.ResultsmiR-146b-5p expression was significantly lower in sepsis patients compared to HD group, with levels in the nonsurvivor group being lower than those in the survivor group. Survival curves for miR-146b-5p indicated that lower levels of miR-146b-5p (<0.272) were associated with a higher mortality rate (HR 3.063). The absolute counts of lymphocytes (Lym), CD3+ T cells, CD4+ T cells, and CD8+ T cells were significantly lower in the sepsis group compared to the HD group. Testing lymphocyte counts at different time points revealed that absolute counts of CD3+ T cells, CD4+ T cells, and CD8+ T cells were consistently lower in the sepsis group across all time intervals. miR-146b-5p levels were predictive of patient prognosis, with the combination of miR-146b-5p and APACHE II scores yielding the highest AUC.ConclusionThe early-stage sepsis-associated downregulation of miR-146b-5p serves as a promising biomarker for severity stratification and prognostic evaluation. The combination of miR-146b-5p with APACHE II scores enhances diagnostic accuracy. Additionally, dynamic monitoring of lymphocyte subsets may facilitate the evaluation of immune status and guide personalized treatment strategies.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1608576/fulldiagnosis and prognosis of sepsisimmunomodulationlymphocyte subsetsmiR-146b-5psepsis |
| spellingShingle | Sha Cheng Jiaojiao Wang Pengfei Wang Xianli Long Huiling Liu Xueyin Li Xiaowei Zhang Xuemei Zhao Hang Sun Chuanxin Wu Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study Frontiers in Immunology diagnosis and prognosis of sepsis immunomodulation lymphocyte subsets miR-146b-5p sepsis |
| title | Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study |
| title_full | Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study |
| title_fullStr | Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study |
| title_full_unstemmed | Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study |
| title_short | Relationship between peripheral blood miR-146b-5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis: a single-center study |
| title_sort | relationship between peripheral blood mir 146b 5p levels and lymphocyte subsets in patients with sepsis and its predictive value for prognosis a single center study |
| topic | diagnosis and prognosis of sepsis immunomodulation lymphocyte subsets miR-146b-5p sepsis |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1608576/full |
| work_keys_str_mv | AT shacheng relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT jiaojiaowang relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT pengfeiwang relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT xianlilong relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT huilingliu relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT xueyinli relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT xiaoweizhang relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT xuemeizhao relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT hangsun relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy AT chuanxinwu relationshipbetweenperipheralbloodmir146b5plevelsandlymphocytesubsetsinpatientswithsepsisanditspredictivevalueforprognosisasinglecenterstudy |