Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize
There is an urgent need to evaluate and optimize use of different sized pollen grains of inducer lines to increase haploid seed production in maize breeding programs. Therefore, this study aimed to evaluate the effect of pollen size on haploid seed production in maize under greenhouse conditions to...
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Institute of Economic Develoment and Social Researches
2023-06-01
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| Series: | ISPEC Journal of Agricultural Sciences |
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| Online Access: | https://www.ispecjournal.com/index.php/ispecjas/article/view/450 |
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| author | Gülizar PINAR Fatih KAHRIMAN |
| author_facet | Gülizar PINAR Fatih KAHRIMAN |
| author_sort | Gülizar PINAR |
| collection | DOAJ |
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There is an urgent need to evaluate and optimize use of different sized pollen grains of inducer lines to increase haploid seed production in maize breeding programs. Therefore, this study aimed to evaluate the effect of pollen size on haploid seed production in maize under greenhouse conditions to characterize pollen samples taken from an inducer maize line by morphological, molecular and spectrophotometric measurements. Three donors and one inducer line were used as the experimental material. The pollen samples of inducer line were collected and filtered into two different sizes (50µ and 100µ) using a special vacuum filtration device. Morphological measurements based on image analysis were made, spectral data were collected in Near Infrared Reflectance (NIR) and Raman spectroscopy devices. The protein bands were examined with SDS-PAGE analysis on the collected pollen and seed samples. Haploid induction rates of the seed samples obtained after pollination of donor materials with sized-pollen samples were determined. The results of the study indicated spectral and molecular differences in the pollen samples separated by 50 and 100µ filters. Haploid induction rate (HIR) values ranged 7.40% -18.9% in the used donors, however higher haploid seed formation was observed in small-sized pollen samples compared to the others.
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| format | Article |
| id | doaj-art-226ee4635b4547e5894db348c0cc55e9 |
| institution | DOAJ |
| issn | 2717-7238 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | Institute of Economic Develoment and Social Researches |
| record_format | Article |
| series | ISPEC Journal of Agricultural Sciences |
| spelling | doaj-art-226ee4635b4547e5894db348c0cc55e92025-08-20T03:01:39ZengInstitute of Economic Develoment and Social ResearchesISPEC Journal of Agricultural Sciences2717-72382023-06-017210.5281/zenodo.8063363Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in MaizeGülizar PINAR0Fatih KAHRIMAN1Çanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, ÇanakkaleÇanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Field Crops, Çanakkale There is an urgent need to evaluate and optimize use of different sized pollen grains of inducer lines to increase haploid seed production in maize breeding programs. Therefore, this study aimed to evaluate the effect of pollen size on haploid seed production in maize under greenhouse conditions to characterize pollen samples taken from an inducer maize line by morphological, molecular and spectrophotometric measurements. Three donors and one inducer line were used as the experimental material. The pollen samples of inducer line were collected and filtered into two different sizes (50µ and 100µ) using a special vacuum filtration device. Morphological measurements based on image analysis were made, spectral data were collected in Near Infrared Reflectance (NIR) and Raman spectroscopy devices. The protein bands were examined with SDS-PAGE analysis on the collected pollen and seed samples. Haploid induction rates of the seed samples obtained after pollination of donor materials with sized-pollen samples were determined. The results of the study indicated spectral and molecular differences in the pollen samples separated by 50 and 100µ filters. Haploid induction rate (HIR) values ranged 7.40% -18.9% in the used donors, however higher haploid seed formation was observed in small-sized pollen samples compared to the others. https://www.ispecjournal.com/index.php/ispecjas/article/view/450PollenploidyproteinnavajoZea mays L. |
| spellingShingle | Gülizar PINAR Fatih KAHRIMAN Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize ISPEC Journal of Agricultural Sciences Pollen ploidy protein navajo Zea mays L. |
| title | Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize |
| title_full | Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize |
| title_fullStr | Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize |
| title_full_unstemmed | Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize |
| title_short | Evaluation of Pollen Samples of Inducer Line with Different Techniques and Pollen Size Effect on Haploid Seed Formation in Maize |
| title_sort | evaluation of pollen samples of inducer line with different techniques and pollen size effect on haploid seed formation in maize |
| topic | Pollen ploidy protein navajo Zea mays L. |
| url | https://www.ispecjournal.com/index.php/ispecjas/article/view/450 |
| work_keys_str_mv | AT gulizarpinar evaluationofpollensamplesofinducerlinewithdifferenttechniquesandpollensizeeffectonhaploidseedformationinmaize AT fatihkahriman evaluationofpollensamplesofinducerlinewithdifferenttechniquesandpollensizeeffectonhaploidseedformationinmaize |