Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference

ObjectThe development of cognitive-enhancing drugs from Ginkgo biloba extract is actively pursued worldwide. This study compares the chemical compositions of different G. biloba extracts and their formulated drugs, highlighting the distinguishing characteristics and potential benefits of optimized G...

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Main Authors: Sunbeom Kwon, Suji Jeong, Seulah Lee
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-08-01
Series:Journal of Pharmacy & Pharmaceutical Sciences
Subjects:
Online Access:https://www.frontierspartnerships.org/articles/10.3389/jpps.2025.14614/full
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author Sunbeom Kwon
Sunbeom Kwon
Suji Jeong
Suji Jeong
Seulah Lee
Seulah Lee
author_facet Sunbeom Kwon
Sunbeom Kwon
Suji Jeong
Suji Jeong
Seulah Lee
Seulah Lee
author_sort Sunbeom Kwon
collection DOAJ
description ObjectThe development of cognitive-enhancing drugs from Ginkgo biloba extract is actively pursued worldwide. This study compares the chemical compositions of different G. biloba extracts and their formulated drugs, highlighting the distinguishing characteristics and potential benefits of optimized G. biloba extract, EGb 761®.MethodsWe analyzed three G. biloba extracts and fifteen formulated drugs using HPLC, principal component analysis, and LC-MS/MS to identify key compositional differences. Molecular docking analysis was conducted to evaluate the binding affinity of the key component with a target protein involved in cognitive enhancement. CYP inhibition assays were performed on selected extracts and their derived products to examine drug-drug interactions.ResultsEGb 761® and its formulated drugs displayed a unique composition, characterized by a significantly higher level of protocatechuic acid (PCA). PCA demonstrated strong interactions with the M1 receptor, acetylcholinesterase, glycogen synthase kinase-3, which are the key targets for cognitive enhancement. CYP inhibition assays indicated that EGb 761® and the drugs derived from EGb 761® had lower inhibitory activity compared to other samples.ConclusionThe high PCA content in EGb 761® may contribute to cognitive benefits. With low CYP inhibition, it suggests minimal interference with drug metabolism, highlighting its potential as a safer cognitive enhancer. Ultimately, this study indicates that the composition of EGb 761® can be effectively leveraged for its pharmacological benefits.
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spelling doaj-art-2269641e8d054ca7bf7d7a6ca0de6d662025-08-26T05:10:02ZengFrontiers Media S.A.Journal of Pharmacy & Pharmaceutical Sciences1482-18262025-08-012810.3389/jpps.2025.1461414614Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interferenceSunbeom Kwon0Sunbeom Kwon1Suji Jeong2Suji Jeong3Seulah Lee4Seulah Lee5Department of Convergent Biotechnology and Advanced Materials Science, College of Life Sciences, Kyung Hee University, Yongin, Republic of KoreaBK21 Interdisciplinary Program in IT-Bio Convergence System, Kyung Hee University, Yongin, Republic of KoreaDepartment of Convergent Biotechnology and Advanced Materials Science, College of Life Sciences, Kyung Hee University, Yongin, Republic of KoreaBK21 Interdisciplinary Program in IT-Bio Convergence System, Kyung Hee University, Yongin, Republic of KoreaDepartment of Convergent Biotechnology and Advanced Materials Science, College of Life Sciences, Kyung Hee University, Yongin, Republic of KoreaBK21 Interdisciplinary Program in IT-Bio Convergence System, Kyung Hee University, Yongin, Republic of KoreaObjectThe development of cognitive-enhancing drugs from Ginkgo biloba extract is actively pursued worldwide. This study compares the chemical compositions of different G. biloba extracts and their formulated drugs, highlighting the distinguishing characteristics and potential benefits of optimized G. biloba extract, EGb 761®.MethodsWe analyzed three G. biloba extracts and fifteen formulated drugs using HPLC, principal component analysis, and LC-MS/MS to identify key compositional differences. Molecular docking analysis was conducted to evaluate the binding affinity of the key component with a target protein involved in cognitive enhancement. CYP inhibition assays were performed on selected extracts and their derived products to examine drug-drug interactions.ResultsEGb 761® and its formulated drugs displayed a unique composition, characterized by a significantly higher level of protocatechuic acid (PCA). PCA demonstrated strong interactions with the M1 receptor, acetylcholinesterase, glycogen synthase kinase-3, which are the key targets for cognitive enhancement. CYP inhibition assays indicated that EGb 761® and the drugs derived from EGb 761® had lower inhibitory activity compared to other samples.ConclusionThe high PCA content in EGb 761® may contribute to cognitive benefits. With low CYP inhibition, it suggests minimal interference with drug metabolism, highlighting its potential as a safer cognitive enhancer. Ultimately, this study indicates that the composition of EGb 761® can be effectively leveraged for its pharmacological benefits.https://www.frontierspartnerships.org/articles/10.3389/jpps.2025.14614/fullEGb 761®protocatechuic acidcognitive enhancementmolecular dockingCYP inhibition
spellingShingle Sunbeom Kwon
Sunbeom Kwon
Suji Jeong
Suji Jeong
Seulah Lee
Seulah Lee
Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
Journal of Pharmacy & Pharmaceutical Sciences
EGb 761®
protocatechuic acid
cognitive enhancement
molecular docking
CYP inhibition
title Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
title_full Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
title_fullStr Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
title_full_unstemmed Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
title_short Optimized Ginkgo biloba extract EGb 761®: boosted therapeutic benefits with minimized CYP enzyme interference
title_sort optimized ginkgo biloba extract egb 761 r boosted therapeutic benefits with minimized cyp enzyme interference
topic EGb 761®
protocatechuic acid
cognitive enhancement
molecular docking
CYP inhibition
url https://www.frontierspartnerships.org/articles/10.3389/jpps.2025.14614/full
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