Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle
Muscarinic receptors types 1 (m1AChR) and 2 (m2AChR) in the preoptic and anterior hypothalamus areas (POA-AHA) were counted, and the effects of blocking these receptors on spontaneous ovulation were analysed throughout the rat oestrous cycle. Rats in each phase of the oestrous cycle were assigned to...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2017/4357080 |
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| author | Yadira L. López-Ramírez Kayro López-Ramírez Isabel Arrieta-Cruz Angélica Flores Luciano Mendoza-Garcés Raúl A. Librado-Osorio Roger Gutiérrez-Juárez Roberto Domínguez María Esther Cruz |
| author_facet | Yadira L. López-Ramírez Kayro López-Ramírez Isabel Arrieta-Cruz Angélica Flores Luciano Mendoza-Garcés Raúl A. Librado-Osorio Roger Gutiérrez-Juárez Roberto Domínguez María Esther Cruz |
| author_sort | Yadira L. López-Ramírez |
| collection | DOAJ |
| description | Muscarinic receptors types 1 (m1AChR) and 2 (m2AChR) in the preoptic and anterior hypothalamus areas (POA-AHA) were counted, and the effects of blocking these receptors on spontaneous ovulation were analysed throughout the rat oestrous cycle. Rats in each phase of the oestrous cycle were assigned to the following experiments: (1) an immunohistochemical study of the number of cells expressing m1AChR or m2AChR in the POA-AHA and (2) analysis of the effects of the unilateral blockade of the m1AChR (pirenzepine, PZP) or m2AChR (methoctramine, MTC) on either side of the POA-AHA on the ovulation rate. The number of m2AChR-immunoreactive cells was significantly higher at 09:00 h on each day of the oestrous cycle in the POA-AHA region, while no changes in the expression profile of m1AChR protein were observed. The ovulation rate in rats treated with PZP on the oestrous day was lower than that in the vehicle group. Animals treated on dioestrous-1 with PZP or MTC had a higher ovulation rate than those in the vehicle group. In contrast, on dioestrous-2, the MTC treatment decreased the ovulation rate. These results suggest that m1AChR or m2AChR in the POA-AHA could participate in the regulation of spontaneous ovulation in rats. |
| format | Article |
| id | doaj-art-2260096ba25b48df8e5ed56ade15dc9c |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-2260096ba25b48df8e5ed56ade15dc9c2025-02-03T01:24:17ZengWileyInternational Journal of Endocrinology1687-83371687-83452017-01-01201710.1155/2017/43570804357080Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous CycleYadira L. López-Ramírez0Kayro López-Ramírez1Isabel Arrieta-Cruz2Angélica Flores3Luciano Mendoza-Garcés4Raúl A. Librado-Osorio5Roger Gutiérrez-Juárez6Roberto Domínguez7María Esther Cruz8Laboratory of Neuroendocrinology, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico City, MexicoLaboratory of Neuroendocrinology, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico City, MexicoDepartment of Basic Research, National Institute of Geriatrics, Mexico City, MexicoLaboratory of Neuroendocrinology, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico City, MexicoDepartment of Basic Research, National Institute of Geriatrics, Mexico City, MexicoDepartment of Basic Research, National Institute of Geriatrics, Mexico City, MexicoDivision of Endocrinology, Department of Medicine, Albert Einstein College of Medicine, Bronx, New York City, NY, USALaboratory of Neuroendocrinology, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico City, MexicoLaboratory of Neuroendocrinology, Biology of Reproduction Research Unit, Facultad de Estudios Superiores Zaragoza, UNAM, Mexico City, MexicoMuscarinic receptors types 1 (m1AChR) and 2 (m2AChR) in the preoptic and anterior hypothalamus areas (POA-AHA) were counted, and the effects of blocking these receptors on spontaneous ovulation were analysed throughout the rat oestrous cycle. Rats in each phase of the oestrous cycle were assigned to the following experiments: (1) an immunohistochemical study of the number of cells expressing m1AChR or m2AChR in the POA-AHA and (2) analysis of the effects of the unilateral blockade of the m1AChR (pirenzepine, PZP) or m2AChR (methoctramine, MTC) on either side of the POA-AHA on the ovulation rate. The number of m2AChR-immunoreactive cells was significantly higher at 09:00 h on each day of the oestrous cycle in the POA-AHA region, while no changes in the expression profile of m1AChR protein were observed. The ovulation rate in rats treated with PZP on the oestrous day was lower than that in the vehicle group. Animals treated on dioestrous-1 with PZP or MTC had a higher ovulation rate than those in the vehicle group. In contrast, on dioestrous-2, the MTC treatment decreased the ovulation rate. These results suggest that m1AChR or m2AChR in the POA-AHA could participate in the regulation of spontaneous ovulation in rats.http://dx.doi.org/10.1155/2017/4357080 |
| spellingShingle | Yadira L. López-Ramírez Kayro López-Ramírez Isabel Arrieta-Cruz Angélica Flores Luciano Mendoza-Garcés Raúl A. Librado-Osorio Roger Gutiérrez-Juárez Roberto Domínguez María Esther Cruz Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle International Journal of Endocrinology |
| title | Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle |
| title_full | Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle |
| title_fullStr | Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle |
| title_full_unstemmed | Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle |
| title_short | Muscarinic Receptors Types 1 and 2 in the Preoptic-Anterior Hypothalamic Areas Regulate Ovulation Unequally in the Rat Oestrous Cycle |
| title_sort | muscarinic receptors types 1 and 2 in the preoptic anterior hypothalamic areas regulate ovulation unequally in the rat oestrous cycle |
| url | http://dx.doi.org/10.1155/2017/4357080 |
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