The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis

In this study, we sequenced and characterized the complete mitochondrial genome of <i>Acorssocheilus spinifer</i>, an endemic stream-dwelling cyprinid species from South China first described in 2006. The mitogenome is 16,591 bp in length and contains the standard set of 37 genes (13 pro...

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Main Authors: Jian Gong, Shi-Qi She, Guang-Fu Liu, Xing-Xing Zhao, Le-Yang Yuan, E Zhang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Fishes
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Online Access:https://www.mdpi.com/2410-3888/10/6/296
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author Jian Gong
Shi-Qi She
Guang-Fu Liu
Xing-Xing Zhao
Le-Yang Yuan
E Zhang
author_facet Jian Gong
Shi-Qi She
Guang-Fu Liu
Xing-Xing Zhao
Le-Yang Yuan
E Zhang
author_sort Jian Gong
collection DOAJ
description In this study, we sequenced and characterized the complete mitochondrial genome of <i>Acorssocheilus spinifer</i>, an endemic stream-dwelling cyprinid species from South China first described in 2006. The mitogenome is 16,591 bp in length and contains the standard set of 37 genes (13 protein-coding genes, 22 tRNA genes, 2 rRNA genes) plus a control region. The genome exhibits typical cyprinid characteristics, with most genes encoded on the H-strand and a nucleotide composition biased toward A + T (55.9%). All tRNA genes display the typical cloverleaf secondary structure, except for tRNA<sup>Ser</sup> (GCU), which lacks the dihydrouridine (DHU) arm. Phylogenetic analysis using complete mitogenomes from 14 <i>Acrossocheilus</i> species revealed that 12 species form a monophyletic assemblage with three distinct clades. Within this framework, <i>A. spinifer</i> clusters closely with <i>A. beijiangensis</i>, supporting previous morphological observations. Our findings provide valuable genetic data for further taxonomic refinement and conservation efforts for Chinese barred species of <i>Cyprinidae</i>.
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institution Kabale University
issn 2410-3888
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publishDate 2025-06-01
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series Fishes
spelling doaj-art-225fc0aa93f7426c91bc4a6abb676d7e2025-08-20T03:24:37ZengMDPI AGFishes2410-38882025-06-0110629610.3390/fishes10060296The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic AnalysisJian Gong0Shi-Qi She1Guang-Fu Liu2Xing-Xing Zhao3Le-Yang Yuan4E Zhang5College of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaZhejiang Museum of Natural History, Hangzhou 310012, ChinaCollege of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaZhejiang Museum of Natural History, Hangzhou 310012, ChinaCollege of Life Sciences, China Jiliang University, Hangzhou 310018, ChinaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, ChinaIn this study, we sequenced and characterized the complete mitochondrial genome of <i>Acorssocheilus spinifer</i>, an endemic stream-dwelling cyprinid species from South China first described in 2006. The mitogenome is 16,591 bp in length and contains the standard set of 37 genes (13 protein-coding genes, 22 tRNA genes, 2 rRNA genes) plus a control region. The genome exhibits typical cyprinid characteristics, with most genes encoded on the H-strand and a nucleotide composition biased toward A + T (55.9%). All tRNA genes display the typical cloverleaf secondary structure, except for tRNA<sup>Ser</sup> (GCU), which lacks the dihydrouridine (DHU) arm. Phylogenetic analysis using complete mitogenomes from 14 <i>Acrossocheilus</i> species revealed that 12 species form a monophyletic assemblage with three distinct clades. Within this framework, <i>A. spinifer</i> clusters closely with <i>A. beijiangensis</i>, supporting previous morphological observations. Our findings provide valuable genetic data for further taxonomic refinement and conservation efforts for Chinese barred species of <i>Cyprinidae</i>.https://www.mdpi.com/2410-3888/10/6/296<i>Acrossocheilus spinifer</i>mitochondrial genomeprotein-coding genesphylogenetic analysis<i>Cyprinidae</i>
spellingShingle Jian Gong
Shi-Qi She
Guang-Fu Liu
Xing-Xing Zhao
Le-Yang Yuan
E Zhang
The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
Fishes
<i>Acrossocheilus spinifer</i>
mitochondrial genome
protein-coding genes
phylogenetic analysis
<i>Cyprinidae</i>
title The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
title_full The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
title_fullStr The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
title_full_unstemmed The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
title_short The Complete Mitochondrial Genome of <i>Acrossocheilus spinifer</i> (Osteichthyes: Cyprinidae) and Its Phylogenetic Analysis
title_sort complete mitochondrial genome of i acrossocheilus spinifer i osteichthyes cyprinidae and its phylogenetic analysis
topic <i>Acrossocheilus spinifer</i>
mitochondrial genome
protein-coding genes
phylogenetic analysis
<i>Cyprinidae</i>
url https://www.mdpi.com/2410-3888/10/6/296
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