Brassinosteroid Enhances Cucumber Stress Tolerance to NaHCO<sub>3</sub> by Modulating Nitrogen Metabolism, Ionic Balance and Phytohormonal Response
Under NaHCO<sub>3</sub> stress, exogenous 24-epibrassinolide (EBR) markedly alleviated Na<sup>+</sup> accumulation in cucumber plants, thereby decreasing the Na<sup>+</sup>/K<sup>+</sup>, Na<sup>+</sup>/Mg<sup>2+</sup>, and Na&l...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/1/80 |
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| Summary: | Under NaHCO<sub>3</sub> stress, exogenous 24-epibrassinolide (EBR) markedly alleviated Na<sup>+</sup> accumulation in cucumber plants, thereby decreasing the Na<sup>+</sup>/K<sup>+</sup>, Na<sup>+</sup>/Mg<sup>2+</sup>, and Na<sup>+</sup>/Ca<sup>2+</sup> ratios. This mitigation was accompanied by elevated concentrations of K<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup>, as well as enhanced expression of the <i>NHX</i> and <i>SOS1</i> genes. In addition, the activities of plasma membrane H<sup>+</sup>-ATPase, vesicular membrane H<sup>+</sup>-ATPase, and vesicular membrane H<sup>+</sup>-PPase were significantly increased, contributing to the maintenance of ionic balance in cucumber plants. NaHCO<sub>3</sub> stress disrupted nitrogen metabolism, as evidenced by reductions in the activities of NR, GS, GOGAT, GOT, and GPT, along with altered GDH activity. These disruptions led to an accumulation of NH<sub>4</sub><sup>+</sup> and substantial decreases in NO<sub>3</sub><sup>−</sup>-N and total nitrogen content. Exogenous EBR alleviated these effects by enhancing the activities of NR, GS, GOGAT, GOT, and GPT, countering the prolonged suppression of GDH activity, and restoring NO<sub>3</sub><sup>−</sup>-N and total nitrogen levels. Consequently, EBR application reduced NH<sub>4</sub><sup>+</sup> toxicity induced by alkali stress. Additionally, NaHCO<sub>3</sub> stress increased ABA accumulation while decreasing IAA and GA<sub>3</sub> content in cucumber seedlings. In contrast, exogenous EBR application elevated IAA and GA<sub>3</sub> levels and increased the IAA/ABA and GA<sub>3</sub>/ABA ratios, thus maintaining hormonal equilibrium under alkali stress. Collectively, these findings highlight that exogenous EBR enhances the alkaline tolerance of cucumber plants by regulating nitrogen metabolism, ion homeostasis, and phytohormonal responses. |
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| ISSN: | 2223-7747 |