Assay of the multiple energy-producing pathways of mammalian cells.
<h4>Background</h4>To elucidate metabolic changes that occur in diabetes, obesity, and cancer, it is important to understand cellular energy metabolism pathways and their alterations in various cells.<h4>Methodology and principal findings</h4>Here we describe a technology for...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-03-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018147&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849725749422981120 |
|---|---|
| author | Barry R Bochner Mark Siri Richard H Huang Shawn Noble Xiang-He Lei Paul A Clemons Bridget K Wagner |
| author_facet | Barry R Bochner Mark Siri Richard H Huang Shawn Noble Xiang-He Lei Paul A Clemons Bridget K Wagner |
| author_sort | Barry R Bochner |
| collection | DOAJ |
| description | <h4>Background</h4>To elucidate metabolic changes that occur in diabetes, obesity, and cancer, it is important to understand cellular energy metabolism pathways and their alterations in various cells.<h4>Methodology and principal findings</h4>Here we describe a technology for simultaneous assessment of cellular energy metabolism pathways. The technology employs a redox dye chemistry specifically coupled to catabolic energy-producing pathways. Using this colorimetric assay, we show that human cancer cell lines from different organ tissues produce distinct profiles of metabolic activity. Further, we show that murine white and brown adipocyte cell lines produce profiles that are distinct from each other as well as from precursor cells undergoing differentiation.<h4>Conclusions</h4>This technology can be employed as a fundamental tool in genotype-phenotype studies to determine changes in cells from shared lineages due to differentiation or mutation. |
| format | Article |
| id | doaj-art-222b35829b6e4d22bd2a7e2cf2e226e6 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2011-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-222b35829b6e4d22bd2a7e2cf2e226e62025-08-20T03:10:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-03-0163e1814710.1371/journal.pone.0018147Assay of the multiple energy-producing pathways of mammalian cells.Barry R BochnerMark SiriRichard H HuangShawn NobleXiang-He LeiPaul A ClemonsBridget K Wagner<h4>Background</h4>To elucidate metabolic changes that occur in diabetes, obesity, and cancer, it is important to understand cellular energy metabolism pathways and their alterations in various cells.<h4>Methodology and principal findings</h4>Here we describe a technology for simultaneous assessment of cellular energy metabolism pathways. The technology employs a redox dye chemistry specifically coupled to catabolic energy-producing pathways. Using this colorimetric assay, we show that human cancer cell lines from different organ tissues produce distinct profiles of metabolic activity. Further, we show that murine white and brown adipocyte cell lines produce profiles that are distinct from each other as well as from precursor cells undergoing differentiation.<h4>Conclusions</h4>This technology can be employed as a fundamental tool in genotype-phenotype studies to determine changes in cells from shared lineages due to differentiation or mutation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018147&type=printable |
| spellingShingle | Barry R Bochner Mark Siri Richard H Huang Shawn Noble Xiang-He Lei Paul A Clemons Bridget K Wagner Assay of the multiple energy-producing pathways of mammalian cells. PLoS ONE |
| title | Assay of the multiple energy-producing pathways of mammalian cells. |
| title_full | Assay of the multiple energy-producing pathways of mammalian cells. |
| title_fullStr | Assay of the multiple energy-producing pathways of mammalian cells. |
| title_full_unstemmed | Assay of the multiple energy-producing pathways of mammalian cells. |
| title_short | Assay of the multiple energy-producing pathways of mammalian cells. |
| title_sort | assay of the multiple energy producing pathways of mammalian cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0018147&type=printable |
| work_keys_str_mv | AT barryrbochner assayofthemultipleenergyproducingpathwaysofmammaliancells AT marksiri assayofthemultipleenergyproducingpathwaysofmammaliancells AT richardhhuang assayofthemultipleenergyproducingpathwaysofmammaliancells AT shawnnoble assayofthemultipleenergyproducingpathwaysofmammaliancells AT xianghelei assayofthemultipleenergyproducingpathwaysofmammaliancells AT paulaclemons assayofthemultipleenergyproducingpathwaysofmammaliancells AT bridgetkwagner assayofthemultipleenergyproducingpathwaysofmammaliancells |