Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition
ABSTRACT Inhalation of biopersistent nanoplastics may have adverse effects on lung health. By varying the acetate content of poly(vinyl acetate‐co‐alcohol) (PVAc), model nanoplastics with different surface hydrophobicity can be produced to study the effects of nanoplastic hydrophobicity in the lung....
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Wiley-VCH
2025-07-01
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| Online Access: | https://doi.org/10.1002/nano.202400154 |
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| author | Anna Babin Morgan Yanira Riffo‐Vasquez Aateka Bodhaniya Stuart A. Jones Ben Forbes Kavitha Sunassee Rafael T. M. deRosales Stephanie Wright Clive Page Lea Ann Dailey |
| author_facet | Anna Babin Morgan Yanira Riffo‐Vasquez Aateka Bodhaniya Stuart A. Jones Ben Forbes Kavitha Sunassee Rafael T. M. deRosales Stephanie Wright Clive Page Lea Ann Dailey |
| author_sort | Anna Babin Morgan |
| collection | DOAJ |
| description | ABSTRACT Inhalation of biopersistent nanoplastics may have adverse effects on lung health. By varying the acetate content of poly(vinyl acetate‐co‐alcohol) (PVAc), model nanoplastics with different surface hydrophobicity can be produced to study the effects of nanoplastic hydrophobicity in the lung. PVAc nanoplastics with a high hydrophobicity, administered by oropharyngeal aspiration to C57BL/6j mice (300 µg; ∼10 mg/kg), show transient pulmonary inflammation which peaks at 24 h post‐administration and resolves by day 7. Hydrophilic PVAc induces no inflammatory effects at the same dose. Pulmonary administration of hydrophilic and hydrophobic PVAc nanoplastics increases the prevalence (∼30%–35%) of distinctive coarsely vacuolated alveolar macrophages over 28 days. Hydrophobic PVAc and silica nanoparticles (control) induce minor increases in collagen deposition, but do not stimulate tissue remodeling to the same extent as a bleomycin model of fibrosis. Longitudinal micro‐CT imaging is explored as a non‐invasive methodology for detection of lung fibrosis. A bespoke image analysis method to quantify high density tissue signal volume correlates moderately well with histopathology‐derived collagen deposition data (R2 = 0.73). In summary, inhaled nanoplastics with high surface hydrophobicity induce transient inflammation following a single administration of 300 µg, an increase in coarsely vacuolated macrophages and mild increases in collagen deposition. |
| format | Article |
| id | doaj-art-2229cbe3a2ef44a897b01ebc8589a85f |
| institution | DOAJ |
| issn | 2688-4011 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Nano Select |
| spelling | doaj-art-2229cbe3a2ef44a897b01ebc8589a85f2025-08-20T02:41:34ZengWiley-VCHNano Select2688-40112025-07-0167n/an/a10.1002/nano.202400154Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen DepositionAnna Babin Morgan0Yanira Riffo‐Vasquez1Aateka Bodhaniya2Stuart A. Jones3Ben Forbes4Kavitha Sunassee5Rafael T. M. deRosales6Stephanie Wright7Clive Page8Lea Ann Dailey9Kingston University Kingston‐upon‐Thames London UKInstitute of Pharmaceutical Science King's College London London UKSchool of Life and Medical Sciences University of Hertfordshire Hatfield, Herts UKInstitute of Pharmaceutical Science King's College London London UKInstitute of Pharmaceutical Science King's College London London UKSchool of Biomedical Engineering and Imaging Sciences King's College London St Thomas Hospital London UKSchool of Biomedical Engineering and Imaging Sciences King's College London St Thomas Hospital London UKSchool of Public Health Imperial College London, Sir Alexander Fleming Building, South Kensington Campus London UKInstitute of Pharmaceutical Science King's College London London UKDepartment of Pharmaceutical Sciences University of Vienna Vienna AustriaABSTRACT Inhalation of biopersistent nanoplastics may have adverse effects on lung health. By varying the acetate content of poly(vinyl acetate‐co‐alcohol) (PVAc), model nanoplastics with different surface hydrophobicity can be produced to study the effects of nanoplastic hydrophobicity in the lung. PVAc nanoplastics with a high hydrophobicity, administered by oropharyngeal aspiration to C57BL/6j mice (300 µg; ∼10 mg/kg), show transient pulmonary inflammation which peaks at 24 h post‐administration and resolves by day 7. Hydrophilic PVAc induces no inflammatory effects at the same dose. Pulmonary administration of hydrophilic and hydrophobic PVAc nanoplastics increases the prevalence (∼30%–35%) of distinctive coarsely vacuolated alveolar macrophages over 28 days. Hydrophobic PVAc and silica nanoparticles (control) induce minor increases in collagen deposition, but do not stimulate tissue remodeling to the same extent as a bleomycin model of fibrosis. Longitudinal micro‐CT imaging is explored as a non‐invasive methodology for detection of lung fibrosis. A bespoke image analysis method to quantify high density tissue signal volume correlates moderately well with histopathology‐derived collagen deposition data (R2 = 0.73). In summary, inhaled nanoplastics with high surface hydrophobicity induce transient inflammation following a single administration of 300 µg, an increase in coarsely vacuolated macrophages and mild increases in collagen deposition.https://doi.org/10.1002/nano.202400154hydrophobicityinflammationlungmacrophagesnanoplastics |
| spellingShingle | Anna Babin Morgan Yanira Riffo‐Vasquez Aateka Bodhaniya Stuart A. Jones Ben Forbes Kavitha Sunassee Rafael T. M. deRosales Stephanie Wright Clive Page Lea Ann Dailey Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition Nano Select hydrophobicity inflammation lung macrophages nanoplastics |
| title | Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition |
| title_full | Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition |
| title_fullStr | Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition |
| title_full_unstemmed | Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition |
| title_short | Intratracheal Administration of Nanoplastics With Varying Surface Hydrophobicity Results in Coarsely Vacuolated Alveolar Macrophages, Transient Respiratory Inflammation, and Mild Collagen Deposition |
| title_sort | intratracheal administration of nanoplastics with varying surface hydrophobicity results in coarsely vacuolated alveolar macrophages transient respiratory inflammation and mild collagen deposition |
| topic | hydrophobicity inflammation lung macrophages nanoplastics |
| url | https://doi.org/10.1002/nano.202400154 |
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