Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province
Objective: It aims to determine the genes characterization of CP1 and CP5 pathogenic factors of E.histolytica parasitic in stool samples of patients in AL- Najaf Al Ashraf Province Methodology: This study was carried out for 6 months in the first of December 2012 till the end of June 2013, The sa...
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University of Kufa, Faculty of Nursing
2013-12-01
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| Series: | Kufa Journal for Nursing Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2490 |
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| author | Haiytham Mohamed Hamady Zainab Ali Hussein |
| author_facet | Haiytham Mohamed Hamady Zainab Ali Hussein |
| author_sort | Haiytham Mohamed Hamady |
| collection | DOAJ |
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Objective: It aims to determine the genes characterization of CP1 and CP5 pathogenic factors of E.histolytica parasitic in stool samples of patients in AL- Najaf Al Ashraf Province
Methodology: This study was carried out for 6 months in the first of December 2012 till the end of June 2013, The samples collected of Al-Sadar teaching hospital, AL-Zahra maternity and children hospital, AL-abasia hospital, AL-Forat hospital, AL-Hakime hospital, AL-Manathera hospital and private clinic in Al- Najaf governorate.
Results: To detect the major virulence factors (V.F.) cysteine proteinase1 (Cp1), cysteine proteinase 5 (CP5) of E.histolytica, Real -Time PCR technique was conducted, by using specific primers for E.histolytica, the results showed that 40 samples out of 162 were positive, out of these positive samples, there were 16 samples positive for C P1, 24 samples were positive for CP5.
Conclusion: CP1 & CP5 is one of the most important virulence factors in E.histolytica such as toxin like activity.
Recommendation: further study development of molecular diagnosis such as real time PCR for other non-pathogenic Entamoeba species which found in human, such as E.moskoviskii in comparison with E.histolytica, because the RT-PCR quantitative and qualitative improved method for the specific diagnosis of E.histolytica infection.
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| format | Article |
| id | doaj-art-221ad0e384e44d73ba94cdf1c8b98a3c |
| institution | OA Journals |
| issn | 2223-4055 2521-8638 |
| language | English |
| publishDate | 2013-12-01 |
| publisher | University of Kufa, Faculty of Nursing |
| record_format | Article |
| series | Kufa Journal for Nursing Sciences |
| spelling | doaj-art-221ad0e384e44d73ba94cdf1c8b98a3c2025-08-20T02:27:32ZengUniversity of Kufa, Faculty of NursingKufa Journal for Nursing Sciences2223-40552521-86382013-12-013310.36321/kjns.vi20133.2490Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf ProvinceHaiytham Mohamed Hamady0Zainab Ali Hussein1University of KufaUniversity of Kufa Objective: It aims to determine the genes characterization of CP1 and CP5 pathogenic factors of E.histolytica parasitic in stool samples of patients in AL- Najaf Al Ashraf Province Methodology: This study was carried out for 6 months in the first of December 2012 till the end of June 2013, The samples collected of Al-Sadar teaching hospital, AL-Zahra maternity and children hospital, AL-abasia hospital, AL-Forat hospital, AL-Hakime hospital, AL-Manathera hospital and private clinic in Al- Najaf governorate. Results: To detect the major virulence factors (V.F.) cysteine proteinase1 (Cp1), cysteine proteinase 5 (CP5) of E.histolytica, Real -Time PCR technique was conducted, by using specific primers for E.histolytica, the results showed that 40 samples out of 162 were positive, out of these positive samples, there were 16 samples positive for C P1, 24 samples were positive for CP5. Conclusion: CP1 & CP5 is one of the most important virulence factors in E.histolytica such as toxin like activity. Recommendation: further study development of molecular diagnosis such as real time PCR for other non-pathogenic Entamoeba species which found in human, such as E.moskoviskii in comparison with E.histolytica, because the RT-PCR quantitative and qualitative improved method for the specific diagnosis of E.histolytica infection. https://journal.uokufa.edu.iq/index.php/kjns/article/view/2490Entamoeba histolytica Real-Time PCRcysteine proteinase |
| spellingShingle | Haiytham Mohamed Hamady Zainab Ali Hussein Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province Kufa Journal for Nursing Sciences Entamoeba histolytica Real-Time PCR cysteine proteinase |
| title | Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province |
| title_full | Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province |
| title_fullStr | Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province |
| title_full_unstemmed | Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province |
| title_short | Real-Time PCR for Detection CP1 & CP5 Virulence Factors of Entamoeba Histolytica in Patients Stool Samples in Al - Najaf Al- Ashraf Province |
| title_sort | real time pcr for detection cp1 cp5 virulence factors of entamoeba histolytica in patients stool samples in al najaf al ashraf province |
| topic | Entamoeba histolytica Real-Time PCR cysteine proteinase |
| url | https://journal.uokufa.edu.iq/index.php/kjns/article/view/2490 |
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