Dual-target electrochemical aptasensor for the simultaneous detection of interleukin-6 and tumor necrosis factor-α in biological fluids
Cytokines are key signaling biomolecules involved in cell growth, immune regulation, inflammation, and cancer-related processes, being valuable biomarkers for diagnosing medical conditions, assessing prognosis, and monitoring treatment efficacy. This study aimed to develop a customized platform enab...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
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| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425001321 |
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| Summary: | Cytokines are key signaling biomolecules involved in cell growth, immune regulation, inflammation, and cancer-related processes, being valuable biomarkers for diagnosing medical conditions, assessing prognosis, and monitoring treatment efficacy. This study aimed to develop a customized platform enabling the simultaneous electrochemical detection of Interleukin-6 (IL-6) and Tumor Necrosis Factor-α (TNF-α) in biological fluids.The platform was fabricated using in-lab printed electrochemical cells. The working electrode was functionalized with Au and Pt nanoparticles to enhance detection sensitivity. The specificity towards the targets was achieved by immobilizing two distinct aptamers, each labeled with a different redox probe, allowing parallel signal readouts and implicitly the simultaneous detection of the two biomarkers. The aptasensor demonstrated simultaneous detection of IL-6 and TNF-α within a linear range of 5–5000 pg/mL, achieving a limit of detection of 1.6 pg/mL. The dual-target aptasensor was validated using real biological samples, including raw saliva and sweat, collected from patients and healthy individuals. Results obtained from the aptasensor were cross-verified with ELISA and validated through statistical analysis. |
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| ISSN: | 2214-1804 |