Protocol for assessing T cell receptor-mediated human T cell cytotoxicity
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering. We describe steps for preparing L and T cells for the assay and incubation...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007068 |
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| author | Kathryne E. Marks Ifeoluwakiisi Adejoorin John Sowerby Deepak A. Rao |
| author_facet | Kathryne E. Marks Ifeoluwakiisi Adejoorin John Sowerby Deepak A. Rao |
| author_sort | Kathryne E. Marks |
| collection | DOAJ |
| description | Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering. We describe steps for preparing L and T cells for the assay and incubation of target cells with effector cells. We detail procedures for assessing cell death by flow cytometry. This protocol can be applied to small numbers of effector cells isolated from patient samples.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-2208603a47b24165baee3a800d5533f1 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-2208603a47b24165baee3a800d5533f12025-01-05T04:28:40ZengElsevierSTAR Protocols2666-16672025-03-0161103541Protocol for assessing T cell receptor-mediated human T cell cytotoxicityKathryne E. Marks0Ifeoluwakiisi Adejoorin1John Sowerby2Deepak A. Rao3Brigham and Women’s Hospital, Division of Rheumatology, Inflammation, and Immunity, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Corresponding authorBrigham and Women’s Hospital, Division of Rheumatology, Inflammation, and Immunity, Boston, MA, USABrigham and Women’s Hospital, Division of Rheumatology, Inflammation, and Immunity, Boston, MA, USA; Harvard Medical School, Boston, MA, USABrigham and Women’s Hospital, Division of Rheumatology, Inflammation, and Immunity, Boston, MA, USA; Harvard Medical School, Boston, MA, USA; Corresponding authorSummary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering. We describe steps for preparing L and T cells for the assay and incubation of target cells with effector cells. We detail procedures for assessing cell death by flow cytometry. This protocol can be applied to small numbers of effector cells isolated from patient samples.For complete details on the use and execution of this protocol, please refer to Wang et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007068Cell cultureCell-based AssaysImmunology |
| spellingShingle | Kathryne E. Marks Ifeoluwakiisi Adejoorin John Sowerby Deepak A. Rao Protocol for assessing T cell receptor-mediated human T cell cytotoxicity STAR Protocols Cell culture Cell-based Assays Immunology |
| title | Protocol for assessing T cell receptor-mediated human T cell cytotoxicity |
| title_full | Protocol for assessing T cell receptor-mediated human T cell cytotoxicity |
| title_fullStr | Protocol for assessing T cell receptor-mediated human T cell cytotoxicity |
| title_full_unstemmed | Protocol for assessing T cell receptor-mediated human T cell cytotoxicity |
| title_short | Protocol for assessing T cell receptor-mediated human T cell cytotoxicity |
| title_sort | protocol for assessing t cell receptor mediated human t cell cytotoxicity |
| topic | Cell culture Cell-based Assays Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007068 |
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