ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo.
Imitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes. Recent studies have also implicated ISWI in the regulation of higher-order chromatin structure, but its role in this...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2007-09-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050232&type=printable |
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| author | Davide F V Corona Giorgia Siriaco Jennifer A Armstrong Natalia Snarskaya Stephanie A McClymont Matthew P Scott John W Tamkun |
| author_facet | Davide F V Corona Giorgia Siriaco Jennifer A Armstrong Natalia Snarskaya Stephanie A McClymont Matthew P Scott John W Tamkun |
| author_sort | Davide F V Corona |
| collection | DOAJ |
| description | Imitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes. Recent studies have also implicated ISWI in the regulation of higher-order chromatin structure, but its role in this process remains poorly understood. To clarify the role of ISWI in vivo, we examined defects in chromosome structure and gene expression resulting from the loss of Iswi function in Drosophila. Consistent with a broad role in transcriptional regulation, the expression of a large number of genes is altered in Iswi mutant larvae. The expression of a dominant-negative form of ISWI leads to dramatic alterations in higher-order chromatin structure, including the apparent decondensation of both mitotic and polytene chromosomes. The loss of ISWI function does not cause obvious defects in nucleosome assembly, but results in a significant reduction in the level of histone H1 associated with chromatin in vivo. These findings suggest that ISWI plays a global role in chromatin compaction in vivo by promoting the association of the linker histone H1 with chromatin. |
| format | Article |
| id | doaj-art-21fd5e7bfc1047ab8f7426656a6682ad |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2007-09-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-21fd5e7bfc1047ab8f7426656a6682ad2025-08-20T02:00:50ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852007-09-0159e23210.1371/journal.pbio.0050232ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo.Davide F V CoronaGiorgia SiriacoJennifer A ArmstrongNatalia SnarskayaStephanie A McClymontMatthew P ScottJohn W TamkunImitation SWI (ISWI) and other ATP-dependent chromatin-remodeling factors play key roles in transcription and other processes by altering the structure and positioning of nucleosomes. Recent studies have also implicated ISWI in the regulation of higher-order chromatin structure, but its role in this process remains poorly understood. To clarify the role of ISWI in vivo, we examined defects in chromosome structure and gene expression resulting from the loss of Iswi function in Drosophila. Consistent with a broad role in transcriptional regulation, the expression of a large number of genes is altered in Iswi mutant larvae. The expression of a dominant-negative form of ISWI leads to dramatic alterations in higher-order chromatin structure, including the apparent decondensation of both mitotic and polytene chromosomes. The loss of ISWI function does not cause obvious defects in nucleosome assembly, but results in a significant reduction in the level of histone H1 associated with chromatin in vivo. These findings suggest that ISWI plays a global role in chromatin compaction in vivo by promoting the association of the linker histone H1 with chromatin.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050232&type=printable |
| spellingShingle | Davide F V Corona Giorgia Siriaco Jennifer A Armstrong Natalia Snarskaya Stephanie A McClymont Matthew P Scott John W Tamkun ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. PLoS Biology |
| title | ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. |
| title_full | ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. |
| title_fullStr | ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. |
| title_full_unstemmed | ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. |
| title_short | ISWI regulates higher-order chromatin structure and histone H1 assembly in vivo. |
| title_sort | iswi regulates higher order chromatin structure and histone h1 assembly in vivo |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.0050232&type=printable |
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