The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23
As the virus continues to spread, the frequency of contact between different parvovirus strains has increased. In recent years, recombinant Muscovy duck parvovirus (rMDPV) has been widely prevalent in China. In 2023, a strain of rMDPV, designated GD-23, was isolated from Guangdong. After isolation a...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2530666 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849430877245800448 |
|---|---|
| author | Mingtian Mao Jiake Li Caiqi Wang Mian Wu ChengGuang Lu Yudong Zhu HuiHui Li Bing Li Meixi Lu Yi Tang |
| author_facet | Mingtian Mao Jiake Li Caiqi Wang Mian Wu ChengGuang Lu Yudong Zhu HuiHui Li Bing Li Meixi Lu Yi Tang |
| author_sort | Mingtian Mao |
| collection | DOAJ |
| description | As the virus continues to spread, the frequency of contact between different parvovirus strains has increased. In recent years, recombinant Muscovy duck parvovirus (rMDPV) has been widely prevalent in China. In 2023, a strain of rMDPV, designated GD-23, was isolated from Guangdong. After isolation and culture, the entire genome of GD-23 was sequenced. A phylogenetic tree was constructed based on the genomic characteristics of GD-23, and recombination events were analysed. The analysis revealed that the virus underwent two recombination events between different parent strains in the P9 promoter region and part of the VP3 region, with highly consistent start and end breakpoints among rMDPV strains. Using AlphaFold3, the VP3 protein conformation of waterfowl parvoviruses (WPVs) was simulated, and potential receptor interaction regions were predicted based on the structural similarities of capsid proteins among viruses of the same genus. Notably, the rMDPV-GD-23 strain exhibited two identical amino acid mutations at distinct sites, which reduced the overall phosphorylation of the viral capsid. Artificial infection experiments in waterfowl demonstrated that the rMDPV-GD-23 strain was pathogenic to multiple waterfowl hosts. This study provides the first evidence that rMDPV possesses cross-host infection capability, a finding that will aid in identifying key amino acid sites on the capsid surface of WPVs. |
| format | Article |
| id | doaj-art-21fc89f1e8694447bf7adb52daa707ba |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-21fc89f1e8694447bf7adb52daa707ba2025-08-20T03:27:51ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2530666The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23Mingtian Mao0Jiake Li1Caiqi Wang2Mian Wu3ChengGuang Lu4Yudong Zhu5HuiHui Li6Bing Li7Meixi Lu8Yi Tang9College of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Animal Science and Technology, Shandong Agricultural University, Tai’an, Shandong, ChinaAs the virus continues to spread, the frequency of contact between different parvovirus strains has increased. In recent years, recombinant Muscovy duck parvovirus (rMDPV) has been widely prevalent in China. In 2023, a strain of rMDPV, designated GD-23, was isolated from Guangdong. After isolation and culture, the entire genome of GD-23 was sequenced. A phylogenetic tree was constructed based on the genomic characteristics of GD-23, and recombination events were analysed. The analysis revealed that the virus underwent two recombination events between different parent strains in the P9 promoter region and part of the VP3 region, with highly consistent start and end breakpoints among rMDPV strains. Using AlphaFold3, the VP3 protein conformation of waterfowl parvoviruses (WPVs) was simulated, and potential receptor interaction regions were predicted based on the structural similarities of capsid proteins among viruses of the same genus. Notably, the rMDPV-GD-23 strain exhibited two identical amino acid mutations at distinct sites, which reduced the overall phosphorylation of the viral capsid. Artificial infection experiments in waterfowl demonstrated that the rMDPV-GD-23 strain was pathogenic to multiple waterfowl hosts. This study provides the first evidence that rMDPV possesses cross-host infection capability, a finding that will aid in identifying key amino acid sites on the capsid surface of WPVs.https://www.tandfonline.com/doi/10.1080/21505594.2025.2530666Recombinant Muscovy duck parvovirus (rMDPV)isolationpathogenicity analysisrecombinationAlphaFold 3amino acid mutations |
| spellingShingle | Mingtian Mao Jiake Li Caiqi Wang Mian Wu ChengGuang Lu Yudong Zhu HuiHui Li Bing Li Meixi Lu Yi Tang The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 Virulence Recombinant Muscovy duck parvovirus (rMDPV) isolation pathogenicity analysis recombination AlphaFold 3 amino acid mutations |
| title | The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 |
| title_full | The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 |
| title_fullStr | The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 |
| title_full_unstemmed | The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 |
| title_short | The study on the molecular characteristics and variation patterns of the recombinant Muscovy duck parvovirus strain GD-23 |
| title_sort | study on the molecular characteristics and variation patterns of the recombinant muscovy duck parvovirus strain gd 23 |
| topic | Recombinant Muscovy duck parvovirus (rMDPV) isolation pathogenicity analysis recombination AlphaFold 3 amino acid mutations |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2530666 |
| work_keys_str_mv | AT mingtianmao thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT jiakeli thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT caiqiwang thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT mianwu thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT chengguanglu thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT yudongzhu thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT huihuili thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT bingli thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT meixilu thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT yitang thestudyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT mingtianmao studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT jiakeli studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT caiqiwang studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT mianwu studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT chengguanglu studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT yudongzhu studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT huihuili studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT bingli studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT meixilu studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 AT yitang studyonthemolecularcharacteristicsandvariationpatternsoftherecombinantmuscovyduckparvovirusstraingd23 |