α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet
High-fat diets (HD) are extensively used in aquaculture due to saving feed cost, reducing protein level, and improve better fish growth and health conditions. However, the harmful impacts of HD, especially on health, limit application of HD in aquaculture. Due to the harmful effect of HD on hepatic...
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Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002960 |
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| author | Hengyang Song Xiangxiang Suo Wanting Huang Yubin Liu Xiaojuan Huang Wenshan Cai Hao Liu Beiping Tan Xiaohui Dong |
| author_facet | Hengyang Song Xiangxiang Suo Wanting Huang Yubin Liu Xiaojuan Huang Wenshan Cai Hao Liu Beiping Tan Xiaohui Dong |
| author_sort | Hengyang Song |
| collection | DOAJ |
| description | High-fat diets (HD) are extensively used in aquaculture due to saving feed cost, reducing protein level, and improve better fish growth and health conditions. However, the harmful impacts of HD, especially on health, limit application of HD in aquaculture. Due to the harmful effect of HD on hepatic health can be alternatively used α-lipoic acid and tea polyphenols, which exhibit great beneficial effect. This study investigated the effects of α-lipoic acid, tea polyphenols, and their combination on growth performance, antioxidant capacity, and inflammation-related indices in hybrid groupers (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) fed a high-fat diet. A basic diet (CK group) with 50 % protein and 17 % lipid content was prepared. α-lipoic acid (LA) and tea polyphenols (TP) were used as additives, and 1000 mg/kg additive was added to the basic diet respectively, and the addition method was LA (LA group), TP (TP group), LA:TP = 1:1 (LA:TP group), LA:TP = 1:2 (LA:2TP group), LA:TP = 2:1 (2LA:TP group). Six experimental diets were fed to hybrid grouper (8.68 ± 0.22 g) for 8 weeks. These results showed that the addition of LA:TP = 1:2 (LA:2TP group) to the diet significantly increased final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) (P < 0.05). Compared with CK, LA, TP, LA:TP and 2LA:TP groups, the serum triglyceride (TG) content in the LA:2TP group was obviously decreased (P < 0.05), and the ratio of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) was enhanced. In addition, the complements C3 and C4 contents in liver of the LA:2TP group were obviously increased, and the total cholesterol (TC) level was significantly decreased (P < 0.05). Meanwhile, compared with the CK group, the serum contents of malondialdehyde (MDA) in each group were markedly reduced (P < 0.05). The results of genes related to inflammation and antioxidation showed that compared with CK, LA, TP and LA:TP groups, the antioxidant genes (sod, cat) and anti-inflammatory cytokine genes (tgf-β, il-10) in liver and head kidney of LA:2TP group were obviously up-regulated (P < 0.05), while those in LA, TP and LA:TP groups were obviously greater than those in CK group (P < 0.05), and the expression level in 2LA:TP group was the lowest (P < 0.05). Correspondingly, the pro-inflammatory cytokine genes (il-6, tnf-α) in the 2LA:TP group were significantly up-regulated and greater than those in other groups (P < 0.05), and the expression level in the LA:2TP group was the lowest (P < 0.05). In conclusion, adding LA:TP = 1:2 can effectively enhance growth performance, improve antioxidant capacity and reduce the harmful effects of HD diet in hybrid grouper. |
| format | Article |
| id | doaj-art-21f8845041dc48eb95d5175869bfe03a |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-21f8845041dc48eb95d5175869bfe03a2025-08-20T04:00:32ZengElsevierAquaculture Reports2352-51342025-09-014310291010.1016/j.aqrep.2025.102910α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-dietHengyang Song0Xiangxiang Suo1Wanting Huang2Yubin Liu3Xiaojuan Huang4Wenshan Cai5Hao Liu6Beiping Tan7Xiaohui Dong8Laboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, ChinaLaboratory of Aquatic Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China; Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Technology Research Center of Guangdong Province, Zhanjiang 524088, China; Correspondence to: Laboratory of Aquatic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China.High-fat diets (HD) are extensively used in aquaculture due to saving feed cost, reducing protein level, and improve better fish growth and health conditions. However, the harmful impacts of HD, especially on health, limit application of HD in aquaculture. Due to the harmful effect of HD on hepatic health can be alternatively used α-lipoic acid and tea polyphenols, which exhibit great beneficial effect. This study investigated the effects of α-lipoic acid, tea polyphenols, and their combination on growth performance, antioxidant capacity, and inflammation-related indices in hybrid groupers (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) fed a high-fat diet. A basic diet (CK group) with 50 % protein and 17 % lipid content was prepared. α-lipoic acid (LA) and tea polyphenols (TP) were used as additives, and 1000 mg/kg additive was added to the basic diet respectively, and the addition method was LA (LA group), TP (TP group), LA:TP = 1:1 (LA:TP group), LA:TP = 1:2 (LA:2TP group), LA:TP = 2:1 (2LA:TP group). Six experimental diets were fed to hybrid grouper (8.68 ± 0.22 g) for 8 weeks. These results showed that the addition of LA:TP = 1:2 (LA:2TP group) to the diet significantly increased final body weight (FBW), weight gain rate (WGR), specific growth rate (SGR) (P < 0.05). Compared with CK, LA, TP, LA:TP and 2LA:TP groups, the serum triglyceride (TG) content in the LA:2TP group was obviously decreased (P < 0.05), and the ratio of high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) was enhanced. In addition, the complements C3 and C4 contents in liver of the LA:2TP group were obviously increased, and the total cholesterol (TC) level was significantly decreased (P < 0.05). Meanwhile, compared with the CK group, the serum contents of malondialdehyde (MDA) in each group were markedly reduced (P < 0.05). The results of genes related to inflammation and antioxidation showed that compared with CK, LA, TP and LA:TP groups, the antioxidant genes (sod, cat) and anti-inflammatory cytokine genes (tgf-β, il-10) in liver and head kidney of LA:2TP group were obviously up-regulated (P < 0.05), while those in LA, TP and LA:TP groups were obviously greater than those in CK group (P < 0.05), and the expression level in 2LA:TP group was the lowest (P < 0.05). Correspondingly, the pro-inflammatory cytokine genes (il-6, tnf-α) in the 2LA:TP group were significantly up-regulated and greater than those in other groups (P < 0.05), and the expression level in the LA:2TP group was the lowest (P < 0.05). In conclusion, adding LA:TP = 1:2 can effectively enhance growth performance, improve antioxidant capacity and reduce the harmful effects of HD diet in hybrid grouper.http://www.sciencedirect.com/science/article/pii/S2352513425002960Hybrid grouperTea polyphenolsAlpha-lipoic acidBiochemical indexes |
| spellingShingle | Hengyang Song Xiangxiang Suo Wanting Huang Yubin Liu Xiaojuan Huang Wenshan Cai Hao Liu Beiping Tan Xiaohui Dong α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet Aquaculture Reports Hybrid grouper Tea polyphenols Alpha-lipoic acid Biochemical indexes |
| title | α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet |
| title_full | α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet |
| title_fullStr | α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet |
| title_full_unstemmed | α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet |
| title_short | α-lipoic acid, tea polyphenols and their combined application affect the growth performance, biochemical indices, and inflammation-related indices of hybrid grouper (♀ Epinephelus fuscoguttatus × ♂ E. lanceolatus) ingests a high fat-diet |
| title_sort | α lipoic acid tea polyphenols and their combined application affect the growth performance biochemical indices and inflammation related indices of hybrid grouper ♀ epinephelus fuscoguttatus ♂ e lanceolatus ingests a high fat diet |
| topic | Hybrid grouper Tea polyphenols Alpha-lipoic acid Biochemical indexes |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425002960 |
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