Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS
IntroductionSeptoria blotch is a globally significant disease, which ranks second in importance after wheat rusts that causes substantial yield losses. The development of Septoria blotch resistant cultivars through molecular approaches is both economical and sustainable strategy to contain the disea...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1524912/full |
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| author | Alma Kokhmetova Nagenahalli Dharmegowda Rathan Deepmala Sehgal Shaukat Ali Yuliya Zeleneva Madina Kumarbayeva Ardak Bolatbekova Gopalareddy Krishnappa Zhenis Keishilov Asia Kokhmetova Kanat Mukhametzhanov Kanat Bakhytuly |
| author_facet | Alma Kokhmetova Nagenahalli Dharmegowda Rathan Deepmala Sehgal Shaukat Ali Yuliya Zeleneva Madina Kumarbayeva Ardak Bolatbekova Gopalareddy Krishnappa Zhenis Keishilov Asia Kokhmetova Kanat Mukhametzhanov Kanat Bakhytuly |
| author_sort | Alma Kokhmetova |
| collection | DOAJ |
| description | IntroductionSeptoria blotch is a globally significant disease, which ranks second in importance after wheat rusts that causes substantial yield losses. The development of Septoria blotch resistant cultivars through molecular approaches is both economical and sustainable strategy to contain the disease.MethodsFor identifying genomic regions associated with resistance to Septoria tritici blotch (STB) and Septoria nodorum blotch (SNB) in wheat, a genome-wide association study (GWAS) was conducted using a diverse panel of 191 spring and winter wheat genotypes. The panel was genotyped using DArTseq™ technology and phenotyped under natural field conditions for three cropping seasons (2019–2020, 2020–2021, and 2021–2022) and under artificially inoculated field conditions for two cropping seasons (2020–2021 and 2021–2022). Additionally, the panel was phenotyped under greenhouse conditions for STB (five mixed isolates in a single experiment) and SNB (four independent isolates and a purified toxin in five different independent experiments).Results and DiscussionGWAS identified nine marker–trait associations (MTAs), including six MTAs for different isolates under greenhouse conditions, two MTAs under natural field conditions, and one MTA under artificially inoculated field conditions. A pleiotropic MTA (100023665) was identified on chromosome 5B governing resistance against SNB isolate Pn Sn2K_USA and SNB purified toxin Pn ToxA_USA and explaining 30.73% and 46.94% of phenotypic variation, respectively. In silico analysis identified important candidate genes belonging to the leucine-rich repeat (LRR) domain superfamily, zinc finger GRF-type transcription factors, potassium transporters, nucleotide-binding site (NBS) domain superfamily, disease resistance protein, P-loop containing nucleoside triphosphate hydrolase, virus X resistance protein, and NB-ARC domains. The stable and major MTAs associated with disease resistant putative candidate genes are valuable for further validation and subsequent application in wheat septoria blotch resistance breeding. |
| format | Article |
| id | doaj-art-21f2104f0bb14776a2df0693d6368df6 |
| institution | OA Journals |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-21f2104f0bb14776a2df0693d6368df62025-08-20T02:15:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-05-011610.3389/fpls.2025.15249121524912Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWASAlma Kokhmetova0Nagenahalli Dharmegowda Rathan1Deepmala Sehgal2Shaukat Ali3Yuliya Zeleneva4Madina Kumarbayeva5Ardak Bolatbekova6Gopalareddy Krishnappa7Zhenis Keishilov8Asia Kokhmetova9Kanat Mukhametzhanov10Kanat Bakhytuly11Laboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanDiscovery Breeding Team, Corteva Agriscience, Hyderabad, Telangana, IndiaSyngenta, Jealott’s Hill International Research Centre, Bracknell, United KingdomAgronomy, Horticulture, and Plant Science Department, South Dakota State University, Brookings, SD, United StatesLaboratory of Mycology and Phytopathology, All Russian Institute of Plant Protection, St. Petersburg-Pushkin, RussiaLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanDepartment of Genetics and Plant Breeding, Indian Council of Agricultural Research (ICAR)-Sugarcane Breeding Institute, Coimbatore, IndiaLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanLaboratory of Breeding and Genetics, Institute of Plant Biology and Biotechnology (IPBB), Almaty, KazakhstanIntroductionSeptoria blotch is a globally significant disease, which ranks second in importance after wheat rusts that causes substantial yield losses. The development of Septoria blotch resistant cultivars through molecular approaches is both economical and sustainable strategy to contain the disease.MethodsFor identifying genomic regions associated with resistance to Septoria tritici blotch (STB) and Septoria nodorum blotch (SNB) in wheat, a genome-wide association study (GWAS) was conducted using a diverse panel of 191 spring and winter wheat genotypes. The panel was genotyped using DArTseq™ technology and phenotyped under natural field conditions for three cropping seasons (2019–2020, 2020–2021, and 2021–2022) and under artificially inoculated field conditions for two cropping seasons (2020–2021 and 2021–2022). Additionally, the panel was phenotyped under greenhouse conditions for STB (five mixed isolates in a single experiment) and SNB (four independent isolates and a purified toxin in five different independent experiments).Results and DiscussionGWAS identified nine marker–trait associations (MTAs), including six MTAs for different isolates under greenhouse conditions, two MTAs under natural field conditions, and one MTA under artificially inoculated field conditions. A pleiotropic MTA (100023665) was identified on chromosome 5B governing resistance against SNB isolate Pn Sn2K_USA and SNB purified toxin Pn ToxA_USA and explaining 30.73% and 46.94% of phenotypic variation, respectively. In silico analysis identified important candidate genes belonging to the leucine-rich repeat (LRR) domain superfamily, zinc finger GRF-type transcription factors, potassium transporters, nucleotide-binding site (NBS) domain superfamily, disease resistance protein, P-loop containing nucleoside triphosphate hydrolase, virus X resistance protein, and NB-ARC domains. The stable and major MTAs associated with disease resistant putative candidate genes are valuable for further validation and subsequent application in wheat septoria blotch resistance breeding.https://www.frontiersin.org/articles/10.3389/fpls.2025.1524912/fullMTAsSeptoria tritici blotchSeptoria nodorum blotchGWAScandidate genes |
| spellingShingle | Alma Kokhmetova Nagenahalli Dharmegowda Rathan Deepmala Sehgal Shaukat Ali Yuliya Zeleneva Madina Kumarbayeva Ardak Bolatbekova Gopalareddy Krishnappa Zhenis Keishilov Asia Kokhmetova Kanat Mukhametzhanov Kanat Bakhytuly Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS Frontiers in Plant Science MTAs Septoria tritici blotch Septoria nodorum blotch GWAS candidate genes |
| title | Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS |
| title_full | Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS |
| title_fullStr | Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS |
| title_full_unstemmed | Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS |
| title_short | Genetic dissection of Septoria tritici blotch and Septoria nodorum blotch resistance in wheat using GWAS |
| title_sort | genetic dissection of septoria tritici blotch and septoria nodorum blotch resistance in wheat using gwas |
| topic | MTAs Septoria tritici blotch Septoria nodorum blotch GWAS candidate genes |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1524912/full |
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