Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy
The application of fluorescence microscopy (FM) for detecting micro- and nano-scale inorganic materials has historically been limited by the lack of specific fluorescent probes. However, recent research has demonstrated that asbestos-binding proteins can act as effective fluorescent probes, signific...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-05-01
|
| Series: | Frontiers in Public Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2025.1568581/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850128619132682240 |
|---|---|
| author | Akio Kuroda |
| author_facet | Akio Kuroda |
| author_sort | Akio Kuroda |
| collection | DOAJ |
| description | The application of fluorescence microscopy (FM) for detecting micro- and nano-scale inorganic materials has historically been limited by the lack of specific fluorescent probes. However, recent research has demonstrated that asbestos-binding proteins can act as effective fluorescent probes, significantly enhancing the sensitivity and selectivity of FM for asbestos fiber detection. This advancement enables the identification of nano-scale fibers at lower magnifications, reducing the labor costs associated with asbestos contamination detection. Based on these advantages, two FM-based methods have been developed: (i) phase-contrast microscopy (PCM)-FM, a differential counting approach fully compatible with PCM-based epidemiological data, and (ii) portable FM, which shows strong potential for rapid on-site asbestos screening. Additionally, FM may enable multicolor labeling and live-cell fluorescent imaging of asbestos, opening new avenues for asbestos research. Despite these advancements, several challenges remain. Fluorescent probes alone cannot definitively identify asbestos, and issues such as cross-reactivity need to be addressed. This review highlights future perspectives and challenges for advancing FM methods in asbestos detection and research. |
| format | Article |
| id | doaj-art-21eed25df51b43c3a7bbf3dfd27e37a4 |
| institution | OA Journals |
| issn | 2296-2565 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj-art-21eed25df51b43c3a7bbf3dfd27e37a42025-08-20T02:33:14ZengFrontiers Media S.A.Frontiers in Public Health2296-25652025-05-011310.3389/fpubh.2025.15685811568581Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopyAkio KurodaThe application of fluorescence microscopy (FM) for detecting micro- and nano-scale inorganic materials has historically been limited by the lack of specific fluorescent probes. However, recent research has demonstrated that asbestos-binding proteins can act as effective fluorescent probes, significantly enhancing the sensitivity and selectivity of FM for asbestos fiber detection. This advancement enables the identification of nano-scale fibers at lower magnifications, reducing the labor costs associated with asbestos contamination detection. Based on these advantages, two FM-based methods have been developed: (i) phase-contrast microscopy (PCM)-FM, a differential counting approach fully compatible with PCM-based epidemiological data, and (ii) portable FM, which shows strong potential for rapid on-site asbestos screening. Additionally, FM may enable multicolor labeling and live-cell fluorescent imaging of asbestos, opening new avenues for asbestos research. Despite these advancements, several challenges remain. Fluorescent probes alone cannot definitively identify asbestos, and issues such as cross-reactivity need to be addressed. This review highlights future perspectives and challenges for advancing FM methods in asbestos detection and research.https://www.frontiersin.org/articles/10.3389/fpubh.2025.1568581/fullasbestosfluorescencefluorescent labelingfluorescence microscopyasbestos-binding protein |
| spellingShingle | Akio Kuroda Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy Frontiers in Public Health asbestos fluorescence fluorescent labeling fluorescence microscopy asbestos-binding protein |
| title | Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| title_full | Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| title_fullStr | Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| title_full_unstemmed | Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| title_short | Fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| title_sort | fluorescent labeling of asbestos fiber for enhanced asbestos detection under fluorescence microscopy |
| topic | asbestos fluorescence fluorescent labeling fluorescence microscopy asbestos-binding protein |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2025.1568581/full |
| work_keys_str_mv | AT akiokuroda fluorescentlabelingofasbestosfiberforenhancedasbestosdetectionunderfluorescencemicroscopy |