Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry
Summary: Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measur...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000048 |
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| author | Johannes Krell Komal Kumar Javarappa Angie Wenedy Andrew L. Frelinger, III Laurent Renia Clarissa Prazeres da Costa Martin Schlegel Percy Knolle Gerhard Schneider Oliver Hayden |
| author_facet | Johannes Krell Komal Kumar Javarappa Angie Wenedy Andrew L. Frelinger, III Laurent Renia Clarissa Prazeres da Costa Martin Schlegel Percy Knolle Gerhard Schneider Oliver Hayden |
| author_sort | Johannes Krell |
| collection | DOAJ |
| description | Summary: Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-21dadfc83591451e9aa3cd89ab3a48fb |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-21dadfc83591451e9aa3cd89ab3a48fb2025-01-26T05:04:56ZengElsevierSTAR Protocols2666-16672025-03-0161103598Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometryJohannes Krell0Komal Kumar Javarappa1Angie Wenedy2Andrew L. Frelinger, III3Laurent Renia4Clarissa Prazeres da Costa5Martin Schlegel6Percy Knolle7Gerhard Schneider8Oliver Hayden9Department of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany; Corresponding authorTUM CREATE Ltd, CREATE Way 10-02, CREATE Tower, SingaporeDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, Germany; TUM CREATE Ltd, CREATE Way 10-02, CREATE Tower, SingaporeCenter for Platelet Research Studies, Dana-Farber/Boston Children’s Cancer and Blood Disorders Center, Harvard Medical School, Boston, MA, USAA∗STAR Infectious Diseases Labs, Agency for Science, Technology and Research (A∗STAR), Singapore, Singapore; Lee Kong Chuan School of Medicine, Nanyang Technological University, Singapore, SingaporeGerman Center for Infection Research (DZIF), partner site Munich, Germany; Center for Global Health, School of Medicine and Health, TUM, München, Germany; Institut für Medizinische Mikrobiologie, Immunologie und Hygiene, School of Medicine and Health, TUM, München, GermanyDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, GermanyInstitute of Molecular Immunology, University Hospital München rechts der Isar, School of Medicine and Health, 81675 München, GermanyDepartment of Anesthesiology and Intensive Care Medicine, TUM University Hospital München, Munich, GermanyHeinz-Nixdorf-Chair of Biomedical Electronics, TranslaTUM, School of Computation, Information and Technology, TUM, Germany; Munich Institute of Biomedical Engineering, TUM, Germany; Corresponding authorSummary: Blood cell aggregates are clinically useful biomarkers in a number of medical disorders. This protocol provides accurate and quantitative analysis of cell aggregates using a small volume of whole blood and imaging flow cytometry. We describe steps for sample collection, staining, and measurement. We then detail gating procedures and analysis of cell morphology. Sample preparation artifacts, activation, and morphological changes of cells are mitigated by omitting erythrocyte lysis and leukocyte isolation while maintaining high-throughput accurate imaging of leukocytes and platelets. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000048Clinical ProtocolFlow CytometryImmunologyMicroscopy |
| spellingShingle | Johannes Krell Komal Kumar Javarappa Angie Wenedy Andrew L. Frelinger, III Laurent Renia Clarissa Prazeres da Costa Martin Schlegel Percy Knolle Gerhard Schneider Oliver Hayden Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry STAR Protocols Clinical Protocol Flow Cytometry Immunology Microscopy |
| title | Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| title_full | Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| title_fullStr | Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| title_full_unstemmed | Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| title_short | Protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| title_sort | protocol for assessing and visualizing cell microaggregate formation in whole blood by imaging flow cytometry |
| topic | Clinical Protocol Flow Cytometry Immunology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000048 |
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