DNA-free CRISPR genome editing in raspberry (Rubus idaeus) protoplast through RNP-mediated transfection

Protoplast-based systems have been utilised in a wide variety of plant species to enable genome editing without chromosomal introgression of foreign DNA into plant genomes. DNA-free genome editing followed by protoplast regeneration allows elite cultivars to be edited without further genetic segrega...

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Bibliographic Details
Main Authors: Ryan Creeth, Andrew Thompson, Zoltan Kevei
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Genome Editing
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2025.1589431/full
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