Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase

Identification of improved signal peptides for heterologous expression in Saccharomyces using a screen that exploits Gaussia luciferase

Abstract A high-throughput and sensitive screen for the improved expression of gene targets in Saccharomyces cerevisiae is described that is based upon the activity of the luciferase from Gaussia princeps. Using the Unspecific Peroxygenase (UPO) from Agrocybe aegerita (AaeUPO) as a model protein, im...

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Bibliographic Details
Main Authors: Ginevra Camboni, Jared Cartwright, Gideon Grogan
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Komagataella phaffii
Pichia pastoris
Gaussia luciferase
Signal peptide
Unspecific peroxygenase
Directed evolution
Online Access:https://doi.org/10.1038/s41598-025-09669-6
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