5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers

Background: The introduction of 5G technology as the latest standard in mobile telecommunications has raised concerns about its potential health effects. Prior studies of earlier generations of radiofrequency electromagnetic fields (RF-EMF) demonstrated narrowband spectral increases in the electroen...

Full description

Saved in:
Bibliographic Details
Main Authors: Georgia Sousouri, Corinne Eicher, Rachele Maria D’Angelo, Marie Billecocq, Thomas Fussinger, Mirjam Studler, Myles Capstick, Niels Kuster, Peter Achermann, Reto Huber, Hans-Peter Landolt
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:NeuroImage
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S105381192500343X
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849417465860194304
author Georgia Sousouri
Corinne Eicher
Rachele Maria D’Angelo
Marie Billecocq
Thomas Fussinger
Mirjam Studler
Myles Capstick
Niels Kuster
Peter Achermann
Reto Huber
Hans-Peter Landolt
author_facet Georgia Sousouri
Corinne Eicher
Rachele Maria D’Angelo
Marie Billecocq
Thomas Fussinger
Mirjam Studler
Myles Capstick
Niels Kuster
Peter Achermann
Reto Huber
Hans-Peter Landolt
author_sort Georgia Sousouri
collection DOAJ
description Background: The introduction of 5G technology as the latest standard in mobile telecommunications has raised concerns about its potential health effects. Prior studies of earlier generations of radiofrequency electromagnetic fields (RF-EMF) demonstrated narrowband spectral increases in the electroencephalographic (EEG) spindle frequency range (11–16 Hz) in non-rapid-eye-movement (NREM) sleep. However, the impact of 5G RF-EMF on sleep remains unexplored. Additionally, RF-EMF can activate l-type voltage-gated calcium channels (LTCC), which have been linked to sleep quality and EEG oscillatory activity. Objective: This study investigates whether the allelic variant rs7304986 in the CACNA1C gene, encoding the α1C subunit of LTCC, modulates 5G RF-EMF effects on EEG spindle activity in NREM sleep. Methods: Thirty-four participants, genotyped for rs7304986 (15 T/C and 19 matched T/T carriers), underwent a double-blind, sham-controlled study with standardized left-hemisphere exposure to two 5G RF-EMF signals (3.6 GHz and 700 MHz) for 30 min before sleep. Sleep spindle activity was analyzed using high-density EEG and the Fitting Oscillations & One Over f (FOOOF) algorithm. Results: T/C carriers reported longer sleep latency compared to T/T carriers. A significant interaction between RF-EMF exposure and rs7304986 genotype was observed, with only 3.6 GHz exposure in T/C carriers inducing a faster spindle center frequency in the central, parietal, and occipital cortex compared to sham. Conclusion: These findings suggest that 3.6 GHz 5G RF-EMF modulates spindle center frequency in NREM sleep in a CACNA1C genotype-dependent manner, implicating LTCC in the physiological response to RF-EMF and underscoring the need for further research into 5G effects on brain health.
format Article
id doaj-art-21cfd554583d4a52b6117bcdfdb5c301
institution Kabale University
issn 1095-9572
language English
publishDate 2025-08-01
publisher Elsevier
record_format Article
series NeuroImage
spelling doaj-art-21cfd554583d4a52b6117bcdfdb5c3012025-08-20T03:32:50ZengElsevierNeuroImage1095-95722025-08-0131712134010.1016/j.neuroimage.2025.1213405G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteersGeorgia Sousouri0Corinne Eicher1Rachele Maria D’Angelo2Marie Billecocq3Thomas Fussinger4Mirjam Studler5Myles Capstick6Niels Kuster7Peter Achermann8Reto Huber9Hans-Peter Landolt10Institute of Pharmacology & Toxicology, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, Switzerland; Department of Psychiatry, Psychotherapy, and Psychosomatics, Psychiatric Hospital of the University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, SwitzerlandIT’IS Foundation, ETH Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, SwitzerlandIT’IS Foundation, ETH Zurich, Zurich, SwitzerlandIT’IS Foundation, ETH Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, Switzerland; Sleep & Health Zurich, University of Zurich, Zurich, SwitzerlandSleep & Health Zurich, University of Zurich, Zurich, Switzerland; University Children's Hospital Zurich, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology & Toxicology, University of Zurich, Zurich, Switzerland; Sleep & Health Zurich, University of Zurich, Zurich, Switzerland; Corresponding author.Background: The introduction of 5G technology as the latest standard in mobile telecommunications has raised concerns about its potential health effects. Prior studies of earlier generations of radiofrequency electromagnetic fields (RF-EMF) demonstrated narrowband spectral increases in the electroencephalographic (EEG) spindle frequency range (11–16 Hz) in non-rapid-eye-movement (NREM) sleep. However, the impact of 5G RF-EMF on sleep remains unexplored. Additionally, RF-EMF can activate l-type voltage-gated calcium channels (LTCC), which have been linked to sleep quality and EEG oscillatory activity. Objective: This study investigates whether the allelic variant rs7304986 in the CACNA1C gene, encoding the α1C subunit of LTCC, modulates 5G RF-EMF effects on EEG spindle activity in NREM sleep. Methods: Thirty-four participants, genotyped for rs7304986 (15 T/C and 19 matched T/T carriers), underwent a double-blind, sham-controlled study with standardized left-hemisphere exposure to two 5G RF-EMF signals (3.6 GHz and 700 MHz) for 30 min before sleep. Sleep spindle activity was analyzed using high-density EEG and the Fitting Oscillations & One Over f (FOOOF) algorithm. Results: T/C carriers reported longer sleep latency compared to T/T carriers. A significant interaction between RF-EMF exposure and rs7304986 genotype was observed, with only 3.6 GHz exposure in T/C carriers inducing a faster spindle center frequency in the central, parietal, and occipital cortex compared to sham. Conclusion: These findings suggest that 3.6 GHz 5G RF-EMF modulates spindle center frequency in NREM sleep in a CACNA1C genotype-dependent manner, implicating LTCC in the physiological response to RF-EMF and underscoring the need for further research into 5G effects on brain health.http://www.sciencedirect.com/science/article/pii/S105381192500343X5G RF-EMFVoltage-gated calcium channelsNREM sleepSleep spindlesBiological mechanism
spellingShingle Georgia Sousouri
Corinne Eicher
Rachele Maria D’Angelo
Marie Billecocq
Thomas Fussinger
Mirjam Studler
Myles Capstick
Niels Kuster
Peter Achermann
Reto Huber
Hans-Peter Landolt
5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
NeuroImage
5G RF-EMF
Voltage-gated calcium channels
NREM sleep
Sleep spindles
Biological mechanism
title 5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
title_full 5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
title_fullStr 5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
title_full_unstemmed 5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
title_short 5G radio-frequency-electromagnetic-field effects on the human sleep electroencephalogram: A randomized controlled study in CACNA1C genotyped volunteers
title_sort 5g radio frequency electromagnetic field effects on the human sleep electroencephalogram a randomized controlled study in cacna1c genotyped volunteers
topic 5G RF-EMF
Voltage-gated calcium channels
NREM sleep
Sleep spindles
Biological mechanism
url http://www.sciencedirect.com/science/article/pii/S105381192500343X
work_keys_str_mv AT georgiasousouri 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT corinneeicher 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT rachelemariadangelo 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT mariebillecocq 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT thomasfussinger 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT mirjamstudler 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT mylescapstick 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT nielskuster 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT peterachermann 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT retohuber 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers
AT hanspeterlandolt 5gradiofrequencyelectromagneticfieldeffectsonthehumansleepelectroencephalogramarandomizedcontrolledstudyincacna1cgenotypedvolunteers