Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine
<b>Background/Objectives:</b> Kiperin Postbiotics, defined as non-viable metabolic products derived from probiotics, have gained attention as potential modulators of cellular responses involved in tissue repair. This study aimed to evaluate the effects of a postbiotic supplement (PS)—com...
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2025-06-01
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| author | Lutfiye Karcioglu Batur Cuneyd Yavas Yağmur Ekenoğlu Merdan Ashabil Aygan |
| author_facet | Lutfiye Karcioglu Batur Cuneyd Yavas Yağmur Ekenoğlu Merdan Ashabil Aygan |
| author_sort | Lutfiye Karcioglu Batur |
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| description | <b>Background/Objectives:</b> Kiperin Postbiotics, defined as non-viable metabolic products derived from probiotics, have gained attention as potential modulators of cellular responses involved in tissue repair. This study aimed to evaluate the effects of a postbiotic supplement (PS)—composed of inactivated strains of <i>Escherichia coli</i>, <i>Lacticaseibacillus rhamnosus</i>, and <i>Lactiplantibacillus plantarum</i>—on fibroblast function, particularly in the context of bacterial secretomes from common pathogenic strains. <b>Methods:</b> Human fibroblast cell lines (HFF-1 and CCD-18Co) were treated with cell-free supernatants (CFS) from <i>E. coli</i> ATCC 25922, <i>Staphylococcus aureus</i> ATCC 29213, and <i>Enterococcus faecalis</i> ATCC 29212, either alone or in combination with the PS. Assessments included cell count, migration (via scratch assay), oxidative stress levels, and expression of immune-related genes (<i>IL-6</i>, <i>IL-10</i>, <i>TNF-α</i>, <i>DRD4</i>). <b>Results:</b> CFS from <i>E. faecalis</i> significantly increased fibroblast counts, whereas <i>E. coli</i> and <i>S. aureus</i> CFS reduced cell counts and elevated oxidative stress. Co-treatment with PS reversed these effects in a strain-dependent manner by lowering oxidative stress and partially restoring cell proliferation. Scratch assays demonstrated enhanced migration in PS-treated fibroblasts. Gene expression analyses revealed no statistically significant changes, though variable trends were observed across treatment groups. <b>Conclusions:</b> PS may mitigate the harmful effects of certain bacterial secretomes while preserving or enhancing beneficial ones. Its ability to reduce oxidative stress and promote fibroblast proliferation and migration suggests a potential pro-regenerative role in vitro. Although gene expression changes were limited, the results offer initial insights into the underlying molecular responses influenced by postbiotic supplementation. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2025-06-01 |
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| series | Biomedicines |
| spelling | doaj-art-21ce4b06d88040dead09783f91a178a42025-08-20T02:24:41ZengMDPI AGBiomedicines2227-90592025-06-01136143010.3390/biomedicines13061430Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative MedicineLutfiye Karcioglu Batur0Cuneyd Yavas1Yağmur Ekenoğlu Merdan2Ashabil Aygan3Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, 34015 Istanbul, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, 34015 Istanbul, TurkeyBiruni University Research Center (B@MER), Biruni University, 34015 Istanbul, TurkeyDepartment of Biology, Faculty of Science, Kahramanmaras Sutcu Imam University, 46050 Kahramanmaras, Turkey<b>Background/Objectives:</b> Kiperin Postbiotics, defined as non-viable metabolic products derived from probiotics, have gained attention as potential modulators of cellular responses involved in tissue repair. This study aimed to evaluate the effects of a postbiotic supplement (PS)—composed of inactivated strains of <i>Escherichia coli</i>, <i>Lacticaseibacillus rhamnosus</i>, and <i>Lactiplantibacillus plantarum</i>—on fibroblast function, particularly in the context of bacterial secretomes from common pathogenic strains. <b>Methods:</b> Human fibroblast cell lines (HFF-1 and CCD-18Co) were treated with cell-free supernatants (CFS) from <i>E. coli</i> ATCC 25922, <i>Staphylococcus aureus</i> ATCC 29213, and <i>Enterococcus faecalis</i> ATCC 29212, either alone or in combination with the PS. Assessments included cell count, migration (via scratch assay), oxidative stress levels, and expression of immune-related genes (<i>IL-6</i>, <i>IL-10</i>, <i>TNF-α</i>, <i>DRD4</i>). <b>Results:</b> CFS from <i>E. faecalis</i> significantly increased fibroblast counts, whereas <i>E. coli</i> and <i>S. aureus</i> CFS reduced cell counts and elevated oxidative stress. Co-treatment with PS reversed these effects in a strain-dependent manner by lowering oxidative stress and partially restoring cell proliferation. Scratch assays demonstrated enhanced migration in PS-treated fibroblasts. Gene expression analyses revealed no statistically significant changes, though variable trends were observed across treatment groups. <b>Conclusions:</b> PS may mitigate the harmful effects of certain bacterial secretomes while preserving or enhancing beneficial ones. Its ability to reduce oxidative stress and promote fibroblast proliferation and migration suggests a potential pro-regenerative role in vitro. Although gene expression changes were limited, the results offer initial insights into the underlying molecular responses influenced by postbiotic supplementation.https://www.mdpi.com/2227-9059/13/6/1430postbioticparaprobioticfibroblasttissue regenerationsmart supplement |
| spellingShingle | Lutfiye Karcioglu Batur Cuneyd Yavas Yağmur Ekenoğlu Merdan Ashabil Aygan Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine Biomedicines postbiotic paraprobiotic fibroblast tissue regeneration smart supplement |
| title | Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine |
| title_full | Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine |
| title_fullStr | Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine |
| title_full_unstemmed | Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine |
| title_short | Kiperin Postbiotic Supplement-Enhanced Bacterial Supernatants Promote Fibroblast Function: Implications for Regenerative Medicine |
| title_sort | kiperin postbiotic supplement enhanced bacterial supernatants promote fibroblast function implications for regenerative medicine |
| topic | postbiotic paraprobiotic fibroblast tissue regeneration smart supplement |
| url | https://www.mdpi.com/2227-9059/13/6/1430 |
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