RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins
The stabilization and processing of salivary transcriptome and proteome biomarkers is a critical challenge due to the ubiquitous nature of nucleases and proteases as well as the inherent instability of these biomarkers. Furthermore, extension of salivary transcriptome and proteome analysis to point-...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-02-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114254 |
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| author | Samantha H. Chiang Gerald A. Thomas Wei Liao Tristan Grogan Robert L. Buck Laurel Fuentes Maha Yakob Mary J. Laughlin Chris Schafer Abu Nazmul-Hossain Fang Wei David Elashoff Paul D. Slowey David T.W. Wong |
| author_facet | Samantha H. Chiang Gerald A. Thomas Wei Liao Tristan Grogan Robert L. Buck Laurel Fuentes Maha Yakob Mary J. Laughlin Chris Schafer Abu Nazmul-Hossain Fang Wei David Elashoff Paul D. Slowey David T.W. Wong |
| author_sort | Samantha H. Chiang |
| collection | DOAJ |
| description | The stabilization and processing of salivary transcriptome and proteome biomarkers is a critical challenge due to the ubiquitous nature of nucleases and proteases as well as the inherent instability of these biomarkers. Furthermore, extension of salivary transcriptome and proteome analysis to point-of-care and remote sites requires the availability of self-administered ambient temperature collection and storage tools. To address these challenges, a self-contained whole saliva collection and extraction system, RNAPro•SAL, has been developed that provides rapid ambient temperature collection along with concurrent processing and stabilization of extracellular RNA (exRNA) and proteins. The system was compared to the University of California, Los Angeles (UCLA) standard clinical collection process (standard operating procedure, SOP). Both systems measured total RNA and protein, and exRNA IL-8, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin and ribosomal protein S9 (RPS9) by qPCR. Proteome analysis was measured by EIA analysis of interleukin-8 (IL-8), and β-actin, as well as total protein. Over 97% of viable cells were removed by both methods. The system compared favorably to the labor-intensive clinical SOP, which requires low-temperature collection and isolation, yielding samples with similar protein and exRNA recovery and stability. |
| format | Article |
| id | doaj-art-21cdb4e435594ca5a802cf84b8029a83 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2015-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-21cdb4e435594ca5a802cf84b8029a832025-08-20T02:25:51ZengTaylor & Francis GroupBioTechniques0736-62051940-98182015-02-01582697610.2144/000114254RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteinsSamantha H. Chiang0Gerald A. Thomas1Wei Liao2Tristan Grogan3Robert L. Buck4Laurel Fuentes5Maha Yakob6Mary J. Laughlin7Chris Schafer8Abu Nazmul-Hossain9Fang Wei10David Elashoff11Paul D. Slowey12David T.W. Wong131UCLA School of Dentistry, Los Angeles, CA2Oasis Diagnostics Corporation, Vancouver, WA1UCLA School of Dentistry, Los Angeles, CA3Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA2Oasis Diagnostics Corporation, Vancouver, WA1UCLA School of Dentistry, Los Angeles, CA1UCLA School of Dentistry, Los Angeles, CA2Oasis Diagnostics Corporation, Vancouver, WA1UCLA School of Dentistry, Los Angeles, CA1UCLA School of Dentistry, Los Angeles, CA1UCLA School of Dentistry, Los Angeles, CA3Department of Biostatistics and Medicine, UCLA School of Public Health, Los Angeles, CA2Oasis Diagnostics Corporation, Vancouver, WA1UCLA School of Dentistry, Los Angeles, CAThe stabilization and processing of salivary transcriptome and proteome biomarkers is a critical challenge due to the ubiquitous nature of nucleases and proteases as well as the inherent instability of these biomarkers. Furthermore, extension of salivary transcriptome and proteome analysis to point-of-care and remote sites requires the availability of self-administered ambient temperature collection and storage tools. To address these challenges, a self-contained whole saliva collection and extraction system, RNAPro•SAL, has been developed that provides rapid ambient temperature collection along with concurrent processing and stabilization of extracellular RNA (exRNA) and proteins. The system was compared to the University of California, Los Angeles (UCLA) standard clinical collection process (standard operating procedure, SOP). Both systems measured total RNA and protein, and exRNA IL-8, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), β-actin and ribosomal protein S9 (RPS9) by qPCR. Proteome analysis was measured by EIA analysis of interleukin-8 (IL-8), and β-actin, as well as total protein. Over 97% of viable cells were removed by both methods. The system compared favorably to the labor-intensive clinical SOP, which requires low-temperature collection and isolation, yielding samples with similar protein and exRNA recovery and stability.https://www.future-science.com/doi/10.2144/000114254saliva diagnostic toolsalivary proteinsalivary extracellular RNAambient temperaturepoint-of-care settings |
| spellingShingle | Samantha H. Chiang Gerald A. Thomas Wei Liao Tristan Grogan Robert L. Buck Laurel Fuentes Maha Yakob Mary J. Laughlin Chris Schafer Abu Nazmul-Hossain Fang Wei David Elashoff Paul D. Slowey David T.W. Wong RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins BioTechniques saliva diagnostic tool salivary protein salivary extracellular RNA ambient temperature point-of-care settings |
| title | RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins |
| title_full | RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins |
| title_fullStr | RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins |
| title_full_unstemmed | RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins |
| title_short | RNAPro•SAL: A device for rapid and standardized collection of saliva RNA and proteins |
| title_sort | rnapro•sal a device for rapid and standardized collection of saliva rna and proteins |
| topic | saliva diagnostic tool salivary protein salivary extracellular RNA ambient temperature point-of-care settings |
| url | https://www.future-science.com/doi/10.2144/000114254 |
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