Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients
Background/Objectives: In a previous study, we described elevated anti-<i>Anisakis</i> IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-<i>Anisakis</i> immunoglobulins in septic patients during ho...
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MDPI AG
2024-11-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/13/4/96 |
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| author | Juan Carlos Andreu-Ballester Amparo Navarro Miguel Angel Arribas Moises Rico Laura Albert Carlos García-Ballesteros Lorena Galindo-Regal Rosa Sorando-Serra Francisca López-Chuliá Federico Peydro Marta Rodero Juan González-Fernández Carmen Cuéllar |
| author_facet | Juan Carlos Andreu-Ballester Amparo Navarro Miguel Angel Arribas Moises Rico Laura Albert Carlos García-Ballesteros Lorena Galindo-Regal Rosa Sorando-Serra Francisca López-Chuliá Federico Peydro Marta Rodero Juan González-Fernández Carmen Cuéllar |
| author_sort | Juan Carlos Andreu-Ballester |
| collection | DOAJ |
| description | Background/Objectives: In a previous study, we described elevated anti-<i>Anisakis</i> IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-<i>Anisakis</i> immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets. Methods: We recruited 80 subjects: 40 patients with sepsis and 40 controls. αβ and γδ T cells were analyzed using flow cytometry. Apoptosis was also assessed, and anti-<i>Anisakis</i> antibodies were measured by ELISA in the sera of patients with sepsis and controls. Results: In the second analysis (7–10 after sepsis evolution), an increase in all specific antibody isotypes was identified in individuals with septic shock, except IgE. The levels of anti-<i>Anisakis</i> IgG and IgA were higher in the subjects with sepsis in the first analysis and continued to increase in the second analysis compared with the healthy control subjects. There was an increase in anti-<i>Anisakis</i> IgG and IgA levels in surviving patients and an increase in IgA levels in non-surviving patients. A rise in specific IgG and IgE levels was noted in the second analysis of patients with sepsis with αβ CD3+ T cell deficiency. Patients without γδ T cell deficiency had increased anti-<i>Anisakis</i> IgA levels 7–10 days after admission. Conclusions: Our results suggest a previous infection by <i>Anisakis</i> that could be involved in the subsequent septic process and be related to patients who have negative cultures in which the pathogen causing sepsis has not been identified. |
| format | Article |
| id | doaj-art-21caac7261b44b4db235ab3b90ea8611 |
| institution | DOAJ |
| issn | 2073-4468 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj-art-21caac7261b44b4db235ab3b90ea86112025-08-20T02:50:56ZengMDPI AGAntibodies2073-44682024-11-011349610.3390/antib13040096Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic PatientsJuan Carlos Andreu-Ballester0Amparo Navarro1Miguel Angel Arribas2Moises Rico3Laura Albert4Carlos García-Ballesteros5Lorena Galindo-Regal6Rosa Sorando-Serra7Francisca López-Chuliá8Federico Peydro9Marta Rodero10Juan González-Fernández11Carmen Cuéllar12FISABIO Foundation–Public Health of Valencia, 46015 Valencia, SpainCritical Care Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainCritical Care Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainCritical Care Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainCritical Care Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainLaboratory of Molecular Biology, Arnau de Vilanova University Hospital, 46015 Valencia, SpainLaboratory of Molecular Biology, Arnau de Vilanova University Hospital, 46015 Valencia, SpainEmergency Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainFISABIO Foundation–Public Health of Valencia, 46015 Valencia, SpainCritical Care Department, Arnau de Vilanova University Hospital, 46015 Valencia, SpainParasitic Immunobiology and Immunomodulation Research Group (INMUNOPAR), Complutense University, 28040 Madrid, SpainParasitic Immunobiology and Immunomodulation Research Group (INMUNOPAR), Complutense University, 28040 Madrid, SpainParasitic Immunobiology and Immunomodulation Research Group (INMUNOPAR), Complutense University, 28040 Madrid, SpainBackground/Objectives: In a previous study, we described elevated anti-<i>Anisakis</i> IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-<i>Anisakis</i> immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets. Methods: We recruited 80 subjects: 40 patients with sepsis and 40 controls. αβ and γδ T cells were analyzed using flow cytometry. Apoptosis was also assessed, and anti-<i>Anisakis</i> antibodies were measured by ELISA in the sera of patients with sepsis and controls. Results: In the second analysis (7–10 after sepsis evolution), an increase in all specific antibody isotypes was identified in individuals with septic shock, except IgE. The levels of anti-<i>Anisakis</i> IgG and IgA were higher in the subjects with sepsis in the first analysis and continued to increase in the second analysis compared with the healthy control subjects. There was an increase in anti-<i>Anisakis</i> IgG and IgA levels in surviving patients and an increase in IgA levels in non-surviving patients. A rise in specific IgG and IgE levels was noted in the second analysis of patients with sepsis with αβ CD3+ T cell deficiency. Patients without γδ T cell deficiency had increased anti-<i>Anisakis</i> IgA levels 7–10 days after admission. Conclusions: Our results suggest a previous infection by <i>Anisakis</i> that could be involved in the subsequent septic process and be related to patients who have negative cultures in which the pathogen causing sepsis has not been identified.https://www.mdpi.com/2073-4468/13/4/96anti-<i>Anisakis</i> antibodiessepsisαβ T cellsγδ T cellsapoptosisELISA |
| spellingShingle | Juan Carlos Andreu-Ballester Amparo Navarro Miguel Angel Arribas Moises Rico Laura Albert Carlos García-Ballesteros Lorena Galindo-Regal Rosa Sorando-Serra Francisca López-Chuliá Federico Peydro Marta Rodero Juan González-Fernández Carmen Cuéllar Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients Antibodies anti-<i>Anisakis</i> antibodies sepsis αβ T cells γδ T cells apoptosis ELISA |
| title | Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients |
| title_full | Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients |
| title_fullStr | Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients |
| title_full_unstemmed | Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients |
| title_short | Increased Levels of Anti-<i>Anisakis</i> Antibodies During Hospital Admission in Septic Patients |
| title_sort | increased levels of anti i anisakis i antibodies during hospital admission in septic patients |
| topic | anti-<i>Anisakis</i> antibodies sepsis αβ T cells γδ T cells apoptosis ELISA |
| url | https://www.mdpi.com/2073-4468/13/4/96 |
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