Optimization Manufacture of Virus- and Tumor-Specific T Cells
Although ex vivo expanded T cells are currently widely used in pre-clinical and clinical trials, the complexity of manufacture remains a major impediment for broader application. In this review we discuss current protocols for the ex vivo expansion of virus- and tumor-specific T cells and describe o...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.4061/2011/434392 |
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| _version_ | 1832563893177679872 |
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| author | Natalia Lapteva Juan F. Vera |
| author_facet | Natalia Lapteva Juan F. Vera |
| author_sort | Natalia Lapteva |
| collection | DOAJ |
| description | Although ex vivo expanded T cells are currently widely used in pre-clinical and clinical trials, the complexity of manufacture remains a major impediment for broader application. In this review we discuss current protocols for the ex vivo expansion of virus- and tumor-specific T cells and describe our experience in manufacture optimization using a gas-permeable static culture flask (G-Rex). This innovative device has revolutionized the manufacture process by allowing us to increase cell yields while decreasing the frequency of cell manipulation and in vitro culture time. It is now being used in good manufacturing practice (GMP) facilities for clinical cell production in our institution as well as many others in the US and worldwide. |
| format | Article |
| id | doaj-art-21b310e1df30454789744d09920937f6 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-21b310e1df30454789744d09920937f62025-02-03T01:12:16ZengWileyStem Cells International1687-966X1687-96782011-01-01201110.4061/2011/434392434392Optimization Manufacture of Virus- and Tumor-Specific T CellsNatalia Lapteva0Juan F. Vera1Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX77030, USACenter for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX77030, USAAlthough ex vivo expanded T cells are currently widely used in pre-clinical and clinical trials, the complexity of manufacture remains a major impediment for broader application. In this review we discuss current protocols for the ex vivo expansion of virus- and tumor-specific T cells and describe our experience in manufacture optimization using a gas-permeable static culture flask (G-Rex). This innovative device has revolutionized the manufacture process by allowing us to increase cell yields while decreasing the frequency of cell manipulation and in vitro culture time. It is now being used in good manufacturing practice (GMP) facilities for clinical cell production in our institution as well as many others in the US and worldwide.http://dx.doi.org/10.4061/2011/434392 |
| spellingShingle | Natalia Lapteva Juan F. Vera Optimization Manufacture of Virus- and Tumor-Specific T Cells Stem Cells International |
| title | Optimization Manufacture of Virus- and Tumor-Specific T Cells |
| title_full | Optimization Manufacture of Virus- and Tumor-Specific T Cells |
| title_fullStr | Optimization Manufacture of Virus- and Tumor-Specific T Cells |
| title_full_unstemmed | Optimization Manufacture of Virus- and Tumor-Specific T Cells |
| title_short | Optimization Manufacture of Virus- and Tumor-Specific T Cells |
| title_sort | optimization manufacture of virus and tumor specific t cells |
| url | http://dx.doi.org/10.4061/2011/434392 |
| work_keys_str_mv | AT natalialapteva optimizationmanufactureofvirusandtumorspecifictcells AT juanfvera optimizationmanufactureofvirusandtumorspecifictcells |