Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L.
Iron oxide nanoparticles (FeONPs) are widely used in various applications, such as biomedicine, environmental remediation, and agriculture. Moreover, FeONPs can affect the production of secondary metabolites in plants, which are compounds that have various biological and pharmacological activities....
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Elsevier
2024-12-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154324004393 |
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| author | Roya Razavizadeh Azama al-Sadat Anwari Amir Hossein Forghani Iman Mirmazloum |
| author_facet | Roya Razavizadeh Azama al-Sadat Anwari Amir Hossein Forghani Iman Mirmazloum |
| author_sort | Roya Razavizadeh |
| collection | DOAJ |
| description | Iron oxide nanoparticles (FeONPs) are widely used in various applications, such as biomedicine, environmental remediation, and agriculture. Moreover, FeONPs can affect the production of secondary metabolites in plants, which are compounds that have various biological and pharmacological activities. Therefore, in this study, the ajwain (Carum copticum L.) seeds were grown in MS medium containing different levels of FeONPs (0, 100, 200, and 400 mg l−1). After four weeks, the effects of FeONPs on growth, photosynthesis pigments, total phenolic, and anthocyanin content, activities of some antioxidant enzyme and secondary metabolites of ajwain) were investigated. The results showed that low levels of FeONPs (especially 200 mg l−1) increased fresh weight (231 %), total chlorophyll (49 %), total phenolics (259 %), anthocyanin content (110 %), as well as thymol (8 %) and γ-terpinene (16 %) content. Conversely, 400 mg l−1 FeONPs reduced root length (60 %) and fresh weight (64 %) was associated with a reduction in CAT activity, phenol (55 %), thymol (22 %), and γ-terpinene (18 %) content compared to ajwain treated with 200 mg l−1 FeONPs. This reduction was probably due to the increase in ROS caused by the low catalase enzyme activity and important essential oils compounds such as thymol and γ-terpinene. Thus, our results indicate that FeONPs at certain levels can be used as a new way for in vitro production of valuable essential oils and antioxidant compounds in ajwain. |
| format | Article |
| id | doaj-art-21b28b5602d849148f58fa7ecff5deab |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-21b28b5602d849148f58fa7ecff5deab2025-08-20T02:49:06ZengElsevierJournal of Agriculture and Food Research2666-15432024-12-011810140210.1016/j.jafr.2024.101402Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L.Roya Razavizadeh0Azama al-Sadat Anwari1Amir Hossein Forghani2Iman Mirmazloum3Department of Biology, Payame Noor University, 193953697, Tehran, Iran; Corresponding authors.Department of Biology, Payame Noor University, 193953697, Tehran, IranDepartment of Biology, Payame Noor University, 193953697, Tehran, Iran; Corresponding authors.Department of Plant Physiology and Plant Ecology, Institute of Agronomy, Hungarian University of Agriculture and Life Sciences, Budapest, 1118, Hungary; Corresponding authors.Iron oxide nanoparticles (FeONPs) are widely used in various applications, such as biomedicine, environmental remediation, and agriculture. Moreover, FeONPs can affect the production of secondary metabolites in plants, which are compounds that have various biological and pharmacological activities. Therefore, in this study, the ajwain (Carum copticum L.) seeds were grown in MS medium containing different levels of FeONPs (0, 100, 200, and 400 mg l−1). After four weeks, the effects of FeONPs on growth, photosynthesis pigments, total phenolic, and anthocyanin content, activities of some antioxidant enzyme and secondary metabolites of ajwain) were investigated. The results showed that low levels of FeONPs (especially 200 mg l−1) increased fresh weight (231 %), total chlorophyll (49 %), total phenolics (259 %), anthocyanin content (110 %), as well as thymol (8 %) and γ-terpinene (16 %) content. Conversely, 400 mg l−1 FeONPs reduced root length (60 %) and fresh weight (64 %) was associated with a reduction in CAT activity, phenol (55 %), thymol (22 %), and γ-terpinene (18 %) content compared to ajwain treated with 200 mg l−1 FeONPs. This reduction was probably due to the increase in ROS caused by the low catalase enzyme activity and important essential oils compounds such as thymol and γ-terpinene. Thus, our results indicate that FeONPs at certain levels can be used as a new way for in vitro production of valuable essential oils and antioxidant compounds in ajwain.http://www.sciencedirect.com/science/article/pii/S2666154324004393AjwainAntioxidant enzymeFeONPsP-cymeneTerpineneThymol |
| spellingShingle | Roya Razavizadeh Azama al-Sadat Anwari Amir Hossein Forghani Iman Mirmazloum Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. Journal of Agriculture and Food Research Ajwain Antioxidant enzyme FeONPs P-cymene Terpinene Thymol |
| title | Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. |
| title_full | Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. |
| title_fullStr | Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. |
| title_full_unstemmed | Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. |
| title_short | Application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured Carum copticum L. |
| title_sort | application of iron oxide nanoparticles improves growth and phytochemical constituents of in vitro cultured carum copticum l |
| topic | Ajwain Antioxidant enzyme FeONPs P-cymene Terpinene Thymol |
| url | http://www.sciencedirect.com/science/article/pii/S2666154324004393 |
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