Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases
Globally, infectious diseases continue to be one of the leading causes of death, even in the face of substantial advancements in basic biological and medical sciences. Diagnostics of life-threatening conditions, including bacteremia, sepsis, meningitis, and encephalitis, is frequently constrained by...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2025-02-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/1187 |
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| author | V. V. Gostev L. I. Gelezova P. S. Chulkova A. A. Avdeeva O. S. Kalinogorskaya V. A. Ageevets I. A. Tsvetkova A. V. Zhuravlev S. V. Sidorenko |
| author_facet | V. V. Gostev L. I. Gelezova P. S. Chulkova A. A. Avdeeva O. S. Kalinogorskaya V. A. Ageevets I. A. Tsvetkova A. V. Zhuravlev S. V. Sidorenko |
| author_sort | V. V. Gostev |
| collection | DOAJ |
| description | Globally, infectious diseases continue to be one of the leading causes of death, even in the face of substantial advancements in basic biological and medical sciences. Diagnostics of life-threatening conditions, including bacteremia, sepsis, meningitis, and encephalitis, is frequently constrained by the low sensitivity associated with culture methods. The application of PCR and various serological methods is constrained by a defined set of specific primers, DNA probes, antibodies, and antigens that are restricted to a limited range of potential pathogens. The accuracy and timeliness of laboratory diagnostics for infectious diseases are critical. In the past decade, a new discipline has emerged — clinical metagenomics, representing a novel approach in medical microbiology. Because clinical metagenomics can identify potentially any pathogen, regardless of its biological nature, it is a hypothesis-free diagnostic approach. The benefits and efficiency of using metagenomic methods of sequencing to identify bacterial, viral, fungal, and parasitic pathogens of infectious diseases in humans are discussed in this review. |
| format | Article |
| id | doaj-art-21afd440a82b41d68d4ce26c01ef2e02 |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2025-02-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-21afd440a82b41d68d4ce26c01ef2e022025-08-20T03:37:11ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902025-02-01699-10617010.37489/0235-2990-2024-69-9-10-61-701065Clinical Metagenomics: a New Approach to Diagnostics of Infectious DiseasesV. V. Gostev0L. I. Gelezova1P. S. Chulkova2A. A. Avdeeva3O. S. Kalinogorskaya4V. A. Ageevets5I. A. Tsvetkova6A. V. Zhuravlev7S. V. Sidorenko8Pediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency ; North-Western State Medical University Named After I. I. MechnikovPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological AgencyPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency ; St. Petersburg State Pediatric Medical University of the Ministry of Health of the Russian FederationPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency ; Clinical Infectious Diseases Hospital named after S. P. BotkinPediatric Research and Clinical Center for Infectious Diseases under the Federal Medical Biological Agency ; North-Western State Medical University Named After I. I. MechnikovGlobally, infectious diseases continue to be one of the leading causes of death, even in the face of substantial advancements in basic biological and medical sciences. Diagnostics of life-threatening conditions, including bacteremia, sepsis, meningitis, and encephalitis, is frequently constrained by the low sensitivity associated with culture methods. The application of PCR and various serological methods is constrained by a defined set of specific primers, DNA probes, antibodies, and antigens that are restricted to a limited range of potential pathogens. The accuracy and timeliness of laboratory diagnostics for infectious diseases are critical. In the past decade, a new discipline has emerged — clinical metagenomics, representing a novel approach in medical microbiology. Because clinical metagenomics can identify potentially any pathogen, regardless of its biological nature, it is a hypothesis-free diagnostic approach. The benefits and efficiency of using metagenomic methods of sequencing to identify bacterial, viral, fungal, and parasitic pathogens of infectious diseases in humans are discussed in this review.https://www.antibiotics-chemotherapy.ru/jour/article/view/1187clinical metagenomicsmetagenomic sequencinginfection diagnosticsmolecular diagnosticstargeted sequencing |
| spellingShingle | V. V. Gostev L. I. Gelezova P. S. Chulkova A. A. Avdeeva O. S. Kalinogorskaya V. A. Ageevets I. A. Tsvetkova A. V. Zhuravlev S. V. Sidorenko Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases Антибиотики и Химиотерапия clinical metagenomics metagenomic sequencing infection diagnostics molecular diagnostics targeted sequencing |
| title | Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases |
| title_full | Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases |
| title_fullStr | Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases |
| title_full_unstemmed | Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases |
| title_short | Clinical Metagenomics: a New Approach to Diagnostics of Infectious Diseases |
| title_sort | clinical metagenomics a new approach to diagnostics of infectious diseases |
| topic | clinical metagenomics metagenomic sequencing infection diagnostics molecular diagnostics targeted sequencing |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/1187 |
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