Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease

We present an overview of recent experimental and computational advances in technology used to characterize the microbiome, with a focus on how these developments improve our understanding of inflammatory bowel disease (IBD). Specifically, we present studies that make use of flow cytometry and metab...

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Main Authors: Alba Boix-Amorós, Hilary Monaco, Elisa Sambataro, Jose C. Clemente
Format: Article
Language:English
Published: Taylor & Francis Group 2022-12-01
Series:Gut Microbes
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Online Access:https://www.tandfonline.com/doi/10.1080/19490976.2022.2107866
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author Alba Boix-Amorós
Hilary Monaco
Elisa Sambataro
Jose C. Clemente
author_facet Alba Boix-Amorós
Hilary Monaco
Elisa Sambataro
Jose C. Clemente
author_sort Alba Boix-Amorós
collection DOAJ
description We present an overview of recent experimental and computational advances in technology used to characterize the microbiome, with a focus on how these developments improve our understanding of inflammatory bowel disease (IBD). Specifically, we present studies that make use of flow cytometry and metabolomics assays to provide a functional characterization of microbial communities. We also describe computational methods for strain-level resolution, temporal series, mycobiome and virome data, co-occurrence networks, and compositional data analysis. In addition, we review novel techniques to therapeutically manipulate the microbiome in IBD. We discuss the benefits and drawbacks of these technologies to increase awareness of specific biases, and to facilitate a more rigorous interpretation of results and their potential clinical application. Finally, we present future lines of research to better characterize the relation between microbial communities and IBD pathogenesis and progression.
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spelling doaj-art-21afbe4334b64401a30f214dc7e53e3a2025-08-20T02:29:56ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2107866Novel technologies to characterize and engineer the microbiome in inflammatory bowel diseaseAlba Boix-Amorós0Hilary Monaco1Elisa Sambataro2Jose C. Clemente3Department of Genetics and Genomic Sciences, Precision Immunology Institute, Icahn School of Medicine at Mount Sinai. New York, NY, USADepartment of Genetics and Genomic Sciences, Precision Immunology Institute, Icahn School of Medicine at Mount Sinai. New York, NY, USADepartment of Biological Sciences, CUNY Hunter College, New York, NY, USADepartment of Genetics and Genomic Sciences, Precision Immunology Institute, Icahn School of Medicine at Mount Sinai. New York, NY, USAWe present an overview of recent experimental and computational advances in technology used to characterize the microbiome, with a focus on how these developments improve our understanding of inflammatory bowel disease (IBD). Specifically, we present studies that make use of flow cytometry and metabolomics assays to provide a functional characterization of microbial communities. We also describe computational methods for strain-level resolution, temporal series, mycobiome and virome data, co-occurrence networks, and compositional data analysis. In addition, we review novel techniques to therapeutically manipulate the microbiome in IBD. We discuss the benefits and drawbacks of these technologies to increase awareness of specific biases, and to facilitate a more rigorous interpretation of results and their potential clinical application. Finally, we present future lines of research to better characterize the relation between microbial communities and IBD pathogenesis and progression.https://www.tandfonline.com/doi/10.1080/19490976.2022.2107866microbiomeIBDtechnologyflow cytometrymetabolomicsnetworks
spellingShingle Alba Boix-Amorós
Hilary Monaco
Elisa Sambataro
Jose C. Clemente
Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
Gut Microbes
microbiome
IBD
technology
flow cytometry
metabolomics
networks
title Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
title_full Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
title_fullStr Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
title_full_unstemmed Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
title_short Novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
title_sort novel technologies to characterize and engineer the microbiome in inflammatory bowel disease
topic microbiome
IBD
technology
flow cytometry
metabolomics
networks
url https://www.tandfonline.com/doi/10.1080/19490976.2022.2107866
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AT elisasambataro noveltechnologiestocharacterizeandengineerthemicrobiomeininflammatoryboweldisease
AT josecclemente noveltechnologiestocharacterizeandengineerthemicrobiomeininflammatoryboweldisease