Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities

The microalgal defense strategies for different white light intensities (70–700 μmol m<sup>−2</sup> s<sup>−1</sup>) were investigated in isolates from unexplored habitats, focusing on photosynthetic performance. <i>Chlorella sorokiniana</i> strain F4 from a Medite...

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Main Authors: Giulio Panicucci, Carolina Chiellini, Cristiana Sbrana, Cristina Echeverría, Lorenzo Guglielminetti, Thais Huarancca Reyes
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Phycology
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Online Access:https://www.mdpi.com/2673-9410/5/2/24
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author Giulio Panicucci
Carolina Chiellini
Cristiana Sbrana
Cristina Echeverría
Lorenzo Guglielminetti
Thais Huarancca Reyes
author_facet Giulio Panicucci
Carolina Chiellini
Cristiana Sbrana
Cristina Echeverría
Lorenzo Guglielminetti
Thais Huarancca Reyes
author_sort Giulio Panicucci
collection DOAJ
description The microalgal defense strategies for different white light intensities (70–700 μmol m<sup>−2</sup> s<sup>−1</sup>) were investigated in isolates from unexplored habitats, focusing on photosynthetic performance. <i>Chlorella sorokiniana</i> strain F4 from a Mediterranean inland swamp and two strains related to <i>Pectinodesmus pectinatus</i> (PEC) and <i>Ettlia pseudoalveolaris</i> (ETI) from an Ecuadorian highland lake were exposed to light over 18 h. The results showed that PSII photochemical efficiency was affected with increasing light due to photoinhibition or photodamage. F4 showed a low threshold of saturation light intensity, after which NPQ was compromised and total antioxidant levels were increased, leading to a reduction in its PSII photochemistry performance. F4 exhibited limited capacity for antennae reorganization in response to light stress. ETI and PEC differed in their photophysiological responses, although they came from the same habitat. ETI maintained high Chl<i>b</i> to Chl<i>a</i> (i.e., large antennae), exhibited sustained energy dissipation, and preserved a high antioxidant pool (i.e., mycosporine-like amino acids) in all lights. Differently, in PEC, NPQ, antennae rearrangement, and reactive oxygen species scavenger pool were induced in a light-dependent manner. This study revealed the complex relationship between light parameters and microalgal physiology affected by environmental constraint adaptation and phylogenetic diversity.
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spelling doaj-art-21aadae20d3146ebbad3bb869eade7522025-08-20T02:21:46ZengMDPI AGPhycology2673-94102025-06-01522410.3390/phycology5020024Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light IntensitiesGiulio Panicucci0Carolina Chiellini1Cristiana Sbrana2Cristina Echeverría3Lorenzo Guglielminetti4Thais Huarancca Reyes5Department of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, ItalyInstitute of Agricultural Biology and Biotechnology, Italian National Research Council, 56124 Pisa, ItalyInstitute of Agricultural Biology and Biotechnology, Italian National Research Council, 56124 Pisa, ItalyDepartment of Biotechnology, Universidad Técnica del Norte, Ibarra 100150, EcuadorDepartment of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, ItalyDepartment of Agriculture, Food and Environment, University of Pisa, 56124 Pisa, ItalyThe microalgal defense strategies for different white light intensities (70–700 μmol m<sup>−2</sup> s<sup>−1</sup>) were investigated in isolates from unexplored habitats, focusing on photosynthetic performance. <i>Chlorella sorokiniana</i> strain F4 from a Mediterranean inland swamp and two strains related to <i>Pectinodesmus pectinatus</i> (PEC) and <i>Ettlia pseudoalveolaris</i> (ETI) from an Ecuadorian highland lake were exposed to light over 18 h. The results showed that PSII photochemical efficiency was affected with increasing light due to photoinhibition or photodamage. F4 showed a low threshold of saturation light intensity, after which NPQ was compromised and total antioxidant levels were increased, leading to a reduction in its PSII photochemistry performance. F4 exhibited limited capacity for antennae reorganization in response to light stress. ETI and PEC differed in their photophysiological responses, although they came from the same habitat. ETI maintained high Chl<i>b</i> to Chl<i>a</i> (i.e., large antennae), exhibited sustained energy dissipation, and preserved a high antioxidant pool (i.e., mycosporine-like amino acids) in all lights. Differently, in PEC, NPQ, antennae rearrangement, and reactive oxygen species scavenger pool were induced in a light-dependent manner. This study revealed the complex relationship between light parameters and microalgal physiology affected by environmental constraint adaptation and phylogenetic diversity.https://www.mdpi.com/2673-9410/5/2/24antioxidants<i>Chlorella sorokiniana</i>chlorophyll fluorescence<i>Ettlia pseudoalveolaris</i>non-photochemical quenching<i>Pectinodesmus pectinatus</i>
spellingShingle Giulio Panicucci
Carolina Chiellini
Cristiana Sbrana
Cristina Echeverría
Lorenzo Guglielminetti
Thais Huarancca Reyes
Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
Phycology
antioxidants
<i>Chlorella sorokiniana</i>
chlorophyll fluorescence
<i>Ettlia pseudoalveolaris</i>
non-photochemical quenching
<i>Pectinodesmus pectinatus</i>
title Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
title_full Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
title_fullStr Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
title_full_unstemmed Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
title_short Phylogenetic Proximity vs. Environmental Adaptation: Exploring Photosynthetic Performances in Mediterranean and Andean Isolated Microalgae Under Different Light Intensities
title_sort phylogenetic proximity vs environmental adaptation exploring photosynthetic performances in mediterranean and andean isolated microalgae under different light intensities
topic antioxidants
<i>Chlorella sorokiniana</i>
chlorophyll fluorescence
<i>Ettlia pseudoalveolaris</i>
non-photochemical quenching
<i>Pectinodesmus pectinatus</i>
url https://www.mdpi.com/2673-9410/5/2/24
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