Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits
This study aims to establish novel protocols for hormonal delivery methods and dosages of Ovopel™ (D-Ala6, Pro9 NEt-mGnRH plus metoclopramide) for spawning induction of female broadhead catfish (Clarias macrocephalus) in control conditions. A total of 84 female breeders (238.63 ± 46.98 g) were place...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006392 |
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| author | Nguyễn Ngọc Quyến Nguyễn Thanh Tâm Thạch Anh Pha Lý Anh Thuật Nguyễn Ngọc Lợi Nguyễn Thị Trúc Quyên László Horváth Tamás Müller |
| author_facet | Nguyễn Ngọc Quyến Nguyễn Thanh Tâm Thạch Anh Pha Lý Anh Thuật Nguyễn Ngọc Lợi Nguyễn Thị Trúc Quyên László Horváth Tamás Müller |
| author_sort | Nguyễn Ngọc Quyến |
| collection | DOAJ |
| description | This study aims to establish novel protocols for hormonal delivery methods and dosages of Ovopel™ (D-Ala6, Pro9 NEt-mGnRH plus metoclopramide) for spawning induction of female broadhead catfish (Clarias macrocephalus) in control conditions. A total of 84 female breeders (238.63 ± 46.98 g) were placed equally into 12 treatments, 7 fish per treatment. The specimens were managed as intramuscular injection (IM), intraperitoneal injection (IP), or ovarian lavage injection (OI). Hormonal induction of ovulation was carried out as follows: three hormonal delivery methods (IM, IP, or OI) in combination with three doses of Ovopel™ (0.5, 1, or 1.5 pellet kg ̶ 1 BW), respectively; the nine treated groups were defined as IM-0.5, IM-1, IM-1.5, IP-0.5, IP-1, IP-1.5, OI-0.5, OI-1, and OI-1.5, respectively. The present results reported that no fish ovulated in the control groups (0.9 % NaCl solution treatments; C-IM, C-IP, and C-OI). In hormone-induced groups, 71.43–100 % of fish ovulated, but no differences were observed in ovulation rates (P > 0.05). The mean latency periods (LT, hours: minutes) indicated significant differences among treated groups (P < 0.05); there was the shortest time in treatment OI-1.5 (7:19 ± 1:04, hours: minutes) and IP-1.5 (7:58 ± 1:01, hours: minutes). The values viz. pseudo-gonadosomatic index (PGSI, %), fertilisation rate (FR, %), hatching rate (HR, %), and survival rate (SR, %) showed considerable individual changes among treatments; nevertheless, there was no statistically significant difference between these average parameters of treated batches (P > 0.05). Taking into account hormone doses (economical consideration) as well as invasive and less-invasive hormone administration, the 0.5-pellet Ovopel™ ovarian lavage treatment is recommended for artificial propagation of C. macrocephalus. |
| format | Article |
| id | doaj-art-21a1e97da9b24a2b9be141272433c1d5 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-21a1e97da9b24a2b9be141272433c1d52025-02-06T05:12:06ZengElsevierAquaculture Reports2352-51342025-03-0140102551Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traitsNguyễn Ngọc Quyến0Nguyễn Thanh Tâm1Thạch Anh Pha2Lý Anh Thuật3Nguyễn Ngọc Lợi4Nguyễn Thị Trúc Quyên5László Horváth6Tamás Müller7Faculty of Fisheries, Nong Lam University, Block 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, VietnamFaculty of Fisheries, Nong Lam University, Block 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, VietnamFaculty of Fisheries, Nong Lam University, Block 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, VietnamFaculty of Fisheries, Nong Lam University, Block 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, VietnamFaculty of Fisheries, Nong Lam University, Block 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, VietnamFaculty of Technology, Dong Nai Technology University, Nguyen Khuyen street, Trang Dai ward, Bien Hoa City, Vietnam; Corresponding author.Department of Freshwater Fish Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agricultural and Life Sciences, Gödöllő 2100, HungaryDepartment of Freshwater Fish Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agricultural and Life Sciences, Gödöllő 2100, HungaryThis study aims to establish novel protocols for hormonal delivery methods and dosages of Ovopel™ (D-Ala6, Pro9 NEt-mGnRH plus metoclopramide) for spawning induction of female broadhead catfish (Clarias macrocephalus) in control conditions. A total of 84 female breeders (238.63 ± 46.98 g) were placed equally into 12 treatments, 7 fish per treatment. The specimens were managed as intramuscular injection (IM), intraperitoneal injection (IP), or ovarian lavage injection (OI). Hormonal induction of ovulation was carried out as follows: three hormonal delivery methods (IM, IP, or OI) in combination with three doses of Ovopel™ (0.5, 1, or 1.5 pellet kg ̶ 1 BW), respectively; the nine treated groups were defined as IM-0.5, IM-1, IM-1.5, IP-0.5, IP-1, IP-1.5, OI-0.5, OI-1, and OI-1.5, respectively. The present results reported that no fish ovulated in the control groups (0.9 % NaCl solution treatments; C-IM, C-IP, and C-OI). In hormone-induced groups, 71.43–100 % of fish ovulated, but no differences were observed in ovulation rates (P > 0.05). The mean latency periods (LT, hours: minutes) indicated significant differences among treated groups (P < 0.05); there was the shortest time in treatment OI-1.5 (7:19 ± 1:04, hours: minutes) and IP-1.5 (7:58 ± 1:01, hours: minutes). The values viz. pseudo-gonadosomatic index (PGSI, %), fertilisation rate (FR, %), hatching rate (HR, %), and survival rate (SR, %) showed considerable individual changes among treatments; nevertheless, there was no statistically significant difference between these average parameters of treated batches (P > 0.05). Taking into account hormone doses (economical consideration) as well as invasive and less-invasive hormone administration, the 0.5-pellet Ovopel™ ovarian lavage treatment is recommended for artificial propagation of C. macrocephalus.http://www.sciencedirect.com/science/article/pii/S2352513424006392Hormonal inductionOvopel™Ovarian lavage methodNovel protocols |
| spellingShingle | Nguyễn Ngọc Quyến Nguyễn Thanh Tâm Thạch Anh Pha Lý Anh Thuật Nguyễn Ngọc Lợi Nguyễn Thị Trúc Quyên László Horváth Tamás Müller Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits Aquaculture Reports Hormonal induction Ovopel™ Ovarian lavage method Novel protocols |
| title | Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits |
| title_full | Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits |
| title_fullStr | Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits |
| title_full_unstemmed | Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits |
| title_short | Optimisation of controlled reproduction protocol in broadhead catfish (Clarias macrocephalus Günther, 1864) using Ovopel™: Effect of different hormonal treatment methods on reproductive traits |
| title_sort | optimisation of controlled reproduction protocol in broadhead catfish clarias macrocephalus gunther 1864 using ovopel™ effect of different hormonal treatment methods on reproductive traits |
| topic | Hormonal induction Ovopel™ Ovarian lavage method Novel protocols |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006392 |
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