Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer
Breast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower i...
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Universitas Gadjah Mada, Yogyakarta
2024-12-01
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| Series: | Indonesian Journal of Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/92817 |
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| author | Fita Nilasari Sofia Mubarika Haryana Dwi Aris Agung Nugrahaningsih Pamungkas Bagus Satriyo |
| author_facet | Fita Nilasari Sofia Mubarika Haryana Dwi Aris Agung Nugrahaningsih Pamungkas Bagus Satriyo |
| author_sort | Fita Nilasari |
| collection | DOAJ |
| description | Breast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower in breast cancer samples from Yogyakarta and Central Java. It is known that miR‐143‐3p functions as a tumor suppressor in breast cancer, and its overexpression corresponds with an increased survival rate. The structure of miRNA is quickly degraded, an enhanced delivery system for miRNA is required. Exosomes are indeed emerging as natural delivery agent. A new approach represents that exosomes will be transfected with mimic‐hsa‐miR‐143‐3p yield an exo‐miR. The research aimed to examine how exo‐miR affects viability, migration, and proliferation using 4T1 cell line. The Exo‐Fect‐based method was used to transfect mimic‐hsa‐miR‐143‐3p into exosomes. The MTT assay, wound healing assay, and colony formation assay were used as functional assay. The MTT assay revealed that 7.5 µL/ 250,000 particles exo‐miR obtained a lower percentage of cell viability (58%) than the control (99.7%). The wound healing assay showed that transfection of 37.5 µL/ 1,250,000 particles exo‐miR was able to suppress migration by the percentage of wound closure (67%) compared to the control (100%). Exo‐miR also had a significant (p < 0.001) effect on colony‐forming abilities, as shown by fewer colonies (32) compared to the control (132). This findings demonstrated that exo‐miR represents a promising targeted approach in cancer therapy. |
| format | Article |
| id | doaj-art-219aaa8d0ffb43b28deffe0ec4bb12b6 |
| institution | DOAJ |
| issn | 0853-8654 2089-2241 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj-art-219aaa8d0ffb43b28deffe0ec4bb12b62025-08-20T03:08:18ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412024-12-0129419019710.22146/ijbiotech.9281737002Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancerFita Nilasari0Sofia Mubarika Haryana1Dwi Aris Agung Nugrahaningsih2Pamungkas Bagus Satriyo3Study Program of Master in Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Histology and Cellular Biology, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Pharmacology and Therapy, Faculty of Medicine Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaBreast cancer represents the highest number of cancer cases in Indonesia, with triple‐negative breast cancer (TNBC) being a common subtype (10–15%). MicroRNAs play a role in cancer epigenetics and contributing as core factors to the disease. The expression of miR‐143‐3p have been found to be lower in breast cancer samples from Yogyakarta and Central Java. It is known that miR‐143‐3p functions as a tumor suppressor in breast cancer, and its overexpression corresponds with an increased survival rate. The structure of miRNA is quickly degraded, an enhanced delivery system for miRNA is required. Exosomes are indeed emerging as natural delivery agent. A new approach represents that exosomes will be transfected with mimic‐hsa‐miR‐143‐3p yield an exo‐miR. The research aimed to examine how exo‐miR affects viability, migration, and proliferation using 4T1 cell line. The Exo‐Fect‐based method was used to transfect mimic‐hsa‐miR‐143‐3p into exosomes. The MTT assay, wound healing assay, and colony formation assay were used as functional assay. The MTT assay revealed that 7.5 µL/ 250,000 particles exo‐miR obtained a lower percentage of cell viability (58%) than the control (99.7%). The wound healing assay showed that transfection of 37.5 µL/ 1,250,000 particles exo‐miR was able to suppress migration by the percentage of wound closure (67%) compared to the control (100%). Exo‐miR also had a significant (p < 0.001) effect on colony‐forming abilities, as shown by fewer colonies (32) compared to the control (132). This findings demonstrated that exo‐miR represents a promising targeted approach in cancer therapy.https://jurnal.ugm.ac.id/ijbiotech/article/view/92817exosomemigrationmimic‐hsa‐mir‐143‐3pproliferationtnbcviability |
| spellingShingle | Fita Nilasari Sofia Mubarika Haryana Dwi Aris Agung Nugrahaningsih Pamungkas Bagus Satriyo Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer Indonesian Journal of Biotechnology exosome migration mimic‐hsa‐mir‐143‐3p proliferation tnbc viability |
| title | Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer |
| title_full | Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer |
| title_fullStr | Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer |
| title_full_unstemmed | Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer |
| title_short | Development of nanocomplex mimic‐hsa‐miR‐143‐3p loaded exosome (exo‐miR) to inhibit viability, migration and proliferation of triple‐negative breast cancer |
| title_sort | development of nanocomplex mimic hsa mir 143 3p loaded exosome exo mir to inhibit viability migration and proliferation of triple negative breast cancer |
| topic | exosome migration mimic‐hsa‐mir‐143‐3p proliferation tnbc viability |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/92817 |
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