Excretion of SARS-CoV-2 through faecal specimens
Coronavirus disease 2019 (COVID-19) has become a pandemic with increasing numbers of cases worldwide. SARS-CoV-2, the causative virus of COVID-19, is mainly transmitted through respiratory droplets or through direct and indirect contact with an infected person. The possibility of potential faecal-or...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2020-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1844551 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850200378686046208 |
|---|---|
| author | Yong Zhang Cao Chen Yang Song Shuangli Zhu Dongyan Wang Hui Zhang Guangyue Han Yuwei Weng Jun Xu Jianan Xu Pengbo Yu Weijia Jiang Xianda Yang Zhongkai Lang Dongmei Yan Yanhai Wang Jingdong Song George Fu Gao Guizhen Wu Wenbo Xu |
| author_facet | Yong Zhang Cao Chen Yang Song Shuangli Zhu Dongyan Wang Hui Zhang Guangyue Han Yuwei Weng Jun Xu Jianan Xu Pengbo Yu Weijia Jiang Xianda Yang Zhongkai Lang Dongmei Yan Yanhai Wang Jingdong Song George Fu Gao Guizhen Wu Wenbo Xu |
| author_sort | Yong Zhang |
| collection | DOAJ |
| description | Coronavirus disease 2019 (COVID-19) has become a pandemic with increasing numbers of cases worldwide. SARS-CoV-2, the causative virus of COVID-19, is mainly transmitted through respiratory droplets or through direct and indirect contact with an infected person. The possibility of potential faecal-oral transmission was investigated in this study. We collected 258 faecal specimens from nine provinces in China and detected the nucleic acid of SARS-CoV-2 using real-time RT–PCR. Vero cells were used to isolate the virus from SARS-CoV-2 nucleic acid positive samples, after which sequencing of Spike gene in eight samples was performed. In all, 93 of 258 (36%) stool samples were positive for SARS-CoV-2 RNA. The positive rates of critical, severe, moderate, and mild patients were 54.4%, 56.1%, 30.8%, and 33.3%, respectively. The content of nucleic acid increased within 2 weeks after the onset of the disease. From the perspective of clinical typing, the nucleic acid can be detected in the faeces of critical patients within two weeks and until four to five weeks in the faeces of severe and mild patients. SARS-CoV-2 was isolated from stool specimens of two severe patients. Four non-synonymous mutations in Spike gene were newly detected in three stool samples. A small number of patients had strong faecal detoxification ability. The live virus in faeces could be an important source of contamination, which may lead to infection and further spread in areas with poor sanitary conditions. The findings of this study have public health significance and they should be considered when formulating disease control strategies. |
| format | Article |
| id | doaj-art-218d4e71906b4aa5a24734ce5fdbcdfa |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-218d4e71906b4aa5a24734ce5fdbcdfa2025-08-20T02:12:20ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01912501250810.1080/22221751.2020.1844551Excretion of SARS-CoV-2 through faecal specimensYong Zhang0Cao Chen1Yang Song2Shuangli Zhu3Dongyan Wang4Hui Zhang5Guangyue Han6Yuwei Weng7Jun Xu8Jianan Xu9Pengbo Yu10Weijia Jiang11Xianda Yang12Zhongkai Lang13Dongmei Yan14Yanhai Wang15Jingdong Song16George Fu Gao17Guizhen Wu18Wenbo Xu19NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.Gansu Provincial Center for Disease Control and Prevention, Lanzhou City, People’s Republic of ChinaHebei Provincial Center for Disease Control and Prevention, Shijiazhuang City, People’s Republic of ChinaFujian Provincial Center for Disease Control and Prevention, Fuzhou City, People’s Republic of ChinaHeilongjiang Provincial Center for Disease Control and Prevention, Harbin City, People’s Republic of ChinaSichuan Provincial Center for Disease Control and Prevention, Chengdu City, People’s Republic of ChinaShaanxiProvincial Center for Disease Control and Prevention, Xi'an City, People’s Republic of China.Guizhou Provincial Center for Disease Control and Prevention, Guiyang City, People’s Republic of ChinaJilin Provincial Center for Disease Control and Prevention, Changchun City, People’s Republic of ChinaChongqing Wanzhou Center for Disease Control and Prevention, Chongqing City, People’s Republic of ChinaNHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.NHC key laboratory for Medical Virology, NHC key laboratory for biosafety. National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, People’s Republic of China.Coronavirus disease 2019 (COVID-19) has become a pandemic with increasing numbers of cases worldwide. SARS-CoV-2, the causative virus of COVID-19, is mainly transmitted through respiratory droplets or through direct and indirect contact with an infected person. The possibility of potential faecal-oral transmission was investigated in this study. We collected 258 faecal specimens from nine provinces in China and detected the nucleic acid of SARS-CoV-2 using real-time RT–PCR. Vero cells were used to isolate the virus from SARS-CoV-2 nucleic acid positive samples, after which sequencing of Spike gene in eight samples was performed. In all, 93 of 258 (36%) stool samples were positive for SARS-CoV-2 RNA. The positive rates of critical, severe, moderate, and mild patients were 54.4%, 56.1%, 30.8%, and 33.3%, respectively. The content of nucleic acid increased within 2 weeks after the onset of the disease. From the perspective of clinical typing, the nucleic acid can be detected in the faeces of critical patients within two weeks and until four to five weeks in the faeces of severe and mild patients. SARS-CoV-2 was isolated from stool specimens of two severe patients. Four non-synonymous mutations in Spike gene were newly detected in three stool samples. A small number of patients had strong faecal detoxification ability. The live virus in faeces could be an important source of contamination, which may lead to infection and further spread in areas with poor sanitary conditions. The findings of this study have public health significance and they should be considered when formulating disease control strategies.https://www.tandfonline.com/doi/10.1080/22221751.2020.1844551COVID-19faecal specimensfaecal-oral transmissionclinical typingpublic health |
| spellingShingle | Yong Zhang Cao Chen Yang Song Shuangli Zhu Dongyan Wang Hui Zhang Guangyue Han Yuwei Weng Jun Xu Jianan Xu Pengbo Yu Weijia Jiang Xianda Yang Zhongkai Lang Dongmei Yan Yanhai Wang Jingdong Song George Fu Gao Guizhen Wu Wenbo Xu Excretion of SARS-CoV-2 through faecal specimens Emerging Microbes and Infections COVID-19 faecal specimens faecal-oral transmission clinical typing public health |
| title | Excretion of SARS-CoV-2 through faecal specimens |
| title_full | Excretion of SARS-CoV-2 through faecal specimens |
| title_fullStr | Excretion of SARS-CoV-2 through faecal specimens |
| title_full_unstemmed | Excretion of SARS-CoV-2 through faecal specimens |
| title_short | Excretion of SARS-CoV-2 through faecal specimens |
| title_sort | excretion of sars cov 2 through faecal specimens |
| topic | COVID-19 faecal specimens faecal-oral transmission clinical typing public health |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1844551 |
| work_keys_str_mv | AT yongzhang excretionofsarscov2throughfaecalspecimens AT caochen excretionofsarscov2throughfaecalspecimens AT yangsong excretionofsarscov2throughfaecalspecimens AT shuanglizhu excretionofsarscov2throughfaecalspecimens AT dongyanwang excretionofsarscov2throughfaecalspecimens AT huizhang excretionofsarscov2throughfaecalspecimens AT guangyuehan excretionofsarscov2throughfaecalspecimens AT yuweiweng excretionofsarscov2throughfaecalspecimens AT junxu excretionofsarscov2throughfaecalspecimens AT jiananxu excretionofsarscov2throughfaecalspecimens AT pengboyu excretionofsarscov2throughfaecalspecimens AT weijiajiang excretionofsarscov2throughfaecalspecimens AT xiandayang excretionofsarscov2throughfaecalspecimens AT zhongkailang excretionofsarscov2throughfaecalspecimens AT dongmeiyan excretionofsarscov2throughfaecalspecimens AT yanhaiwang excretionofsarscov2throughfaecalspecimens AT jingdongsong excretionofsarscov2throughfaecalspecimens AT georgefugao excretionofsarscov2throughfaecalspecimens AT guizhenwu excretionofsarscov2throughfaecalspecimens AT wenboxu excretionofsarscov2throughfaecalspecimens |