Functional Analysis of the <i>ZmPR5</i> Gene Related to Resistance Against <i>Fusarium verticillioides</i> in Maize
In this study, the gene <i>ZmPR5</i>, associated with resistance to ear rot, was identified through transcriptome data analysis of the maize inbred line J1259. The gene was subsequently cloned and its function was investigated. The <i>ZmPR5</i> gene comprises an open reading...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
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| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/5/737 |
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| Summary: | In this study, the gene <i>ZmPR5</i>, associated with resistance to ear rot, was identified through transcriptome data analysis of the maize inbred line J1259. The gene was subsequently cloned and its function was investigated. The <i>ZmPR5</i> gene comprises an open reading frame of 525 base pairs, encoding a protein of 175 amino acids. <i>ZmPR5</i> was overexpressed in Arabidopsis and the <i>ZmPR5EMS</i> mutant in maize, and they were subjected to q-PCR and measurements of antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, and chlorophyll content. The results indicate that the expression of <i>ZmPR5</i> is up-regulated upon infection by <i>Fusarium verticillioides</i>, with significant differences observed in the activities of POD, SOD, CAT, MDA, electrical conductivity, and chlorophyll content. The study found that <i>ZmPR5</i> is localized in the nucleus and interacts with <i>Zm00001d020492 (WRKY53)</i> and <i>Zm00001d042140 (glucA)</i>. Trypan blue staining revealed that the stained area in the <i>ZmPR5EMS</i> mutant of maize was significantly larger than that in B73. The expression of <i>ZmPR5</i> is closely associated with resistance to maize ear rot. |
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| ISSN: | 2223-7747 |