Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy

Diabetic retinopathy (DR) is a leading cause of vision impairment worldwide, necessitating early detection and personalized treatment strategies. Liquid biopsy, a minimally invasive diagnostic tool, offers significant advantages over traditional methods by enabling analysis of biofluids such as bloo...

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Main Authors: Qing Chen, Yi Chen, Kefan Mou, Ming Zhang
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Biomedicines
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Online Access:https://www.mdpi.com/2227-9059/13/6/1306
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author Qing Chen
Yi Chen
Kefan Mou
Ming Zhang
author_facet Qing Chen
Yi Chen
Kefan Mou
Ming Zhang
author_sort Qing Chen
collection DOAJ
description Diabetic retinopathy (DR) is a leading cause of vision impairment worldwide, necessitating early detection and personalized treatment strategies. Liquid biopsy, a minimally invasive diagnostic tool, offers significant advantages over traditional methods by enabling analysis of biofluids such as blood, tears, aqueous humor, and vitreous humor. This review highlights the advances in liquid biopsy techniques, sample collection methods and their applications in protein detection and metabolomics analysis for DR. It also explores the key protein biomarkers, including vascular endothelial growth factor (VEGF), inflammatory cytokines, and matrix metalloproteinases (MMPs), and investigates the associations between different biofluids. Metabolomics of liquid biopsy is emphasized for its role in identifying metabolic biomarkers linked to DR pathogenesis, providing new insights into disease mechanisms and personalized interventions. The challenges of liquid biopsy, such as technical limitations and the need for standardization, are also discussed. Advances in computational tools, bioinformatics, and artificial intelligence are supposed to further enhance multi-omics integration, thereby improving precision medicine in DR care. This comprehensive review brings attention to the transformative potential of liquid biopsy in DR diagnosis and management, with implications for broader ophthalmic and systemic diseases.
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spelling doaj-art-21847a164824456ebce26e4037eef1c22025-08-20T03:32:32ZengMDPI AGBiomedicines2227-90592025-05-01136130610.3390/biomedicines13061306Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic RetinopathyQing Chen0Yi Chen1Kefan Mou2Ming Zhang3Department of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, ChinaDiabetic retinopathy (DR) is a leading cause of vision impairment worldwide, necessitating early detection and personalized treatment strategies. Liquid biopsy, a minimally invasive diagnostic tool, offers significant advantages over traditional methods by enabling analysis of biofluids such as blood, tears, aqueous humor, and vitreous humor. This review highlights the advances in liquid biopsy techniques, sample collection methods and their applications in protein detection and metabolomics analysis for DR. It also explores the key protein biomarkers, including vascular endothelial growth factor (VEGF), inflammatory cytokines, and matrix metalloproteinases (MMPs), and investigates the associations between different biofluids. Metabolomics of liquid biopsy is emphasized for its role in identifying metabolic biomarkers linked to DR pathogenesis, providing new insights into disease mechanisms and personalized interventions. The challenges of liquid biopsy, such as technical limitations and the need for standardization, are also discussed. Advances in computational tools, bioinformatics, and artificial intelligence are supposed to further enhance multi-omics integration, thereby improving precision medicine in DR care. This comprehensive review brings attention to the transformative potential of liquid biopsy in DR diagnosis and management, with implications for broader ophthalmic and systemic diseases.https://www.mdpi.com/2227-9059/13/6/1306diabetesdiabetic retinopathycomplicationsvascular endothelial growth factorbiomarkersliquid biopsy
spellingShingle Qing Chen
Yi Chen
Kefan Mou
Ming Zhang
Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
Biomedicines
diabetes
diabetic retinopathy
complications
vascular endothelial growth factor
biomarkers
liquid biopsy
title Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
title_full Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
title_fullStr Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
title_full_unstemmed Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
title_short Liquid Biopsy Combined with Multi-Omics Approaches in Diagnosis, Management, and Progression of Diabetic Retinopathy
title_sort liquid biopsy combined with multi omics approaches in diagnosis management and progression of diabetic retinopathy
topic diabetes
diabetic retinopathy
complications
vascular endothelial growth factor
biomarkers
liquid biopsy
url https://www.mdpi.com/2227-9059/13/6/1306
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