Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides
Abstract Background Synthetic human milk oligosaccharides (HMOs) are used to supplement infant formula despite limited understanding of their impact on the post-weaned developing gut microbiota. Here, we assess the influence of 0.5 g/L 2-fucosyllactose (2’FL) and 4.0 g/L pooled HMOs (pHMOs) on the c...
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BMC
2025-02-01
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| Series: | Microbiome |
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| Online Access: | https://doi.org/10.1186/s40168-025-02034-9 |
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| author | Simone Renwick Annalee Furst Mikael Knip on behalf of the DIABIMMUNE Study Group Lars Bode Jayne S. Danska Emma Allen-Vercoe |
| author_facet | Simone Renwick Annalee Furst Mikael Knip on behalf of the DIABIMMUNE Study Group Lars Bode Jayne S. Danska Emma Allen-Vercoe |
| author_sort | Simone Renwick |
| collection | DOAJ |
| description | Abstract Background Synthetic human milk oligosaccharides (HMOs) are used to supplement infant formula despite limited understanding of their impact on the post-weaned developing gut microbiota. Here, we assess the influence of 0.5 g/L 2-fucosyllactose (2’FL) and 4.0 g/L pooled HMOs (pHMOs) on the composition and activity of cultured fecal-derived microbial communities from seven healthy young children. Results Exposure to pHMOs induced significant shifts in both the microbial community composition and metabolic output, including an increased abundance of several genera, notably Bacteroides, and the production of health-associated metabolites. In contrast, 2’FL alone did not lead to substantial changes in the communities. A total of 330 bacterial isolates, spanning 157 species, were cultured from these communities and individually evaluated for their responses to HMOs. Over 100 non-Bifidobacterium species showed enhanced growth upon pHMOs treatment and a high degree of intraspecies variation in HMO metabolism was observed. Conclusion Our study provides valuable insight into the health-enhancing properties of HMOs while highlighting the need for future research into the efficacy of incorporating individual structures into infant formula, particularly when aiming to modulate the gut microbiota. Video Abstract |
| format | Article |
| id | doaj-art-2182f5dda03e46ad9582142570eccdad |
| institution | Kabale University |
| issn | 2049-2618 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj-art-2182f5dda03e46ad9582142570eccdad2025-02-09T12:46:49ZengBMCMicrobiome2049-26182025-02-0113111310.1186/s40168-025-02034-9Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharidesSimone Renwick0Annalee Furst1Mikael Knip2on behalf of the DIABIMMUNE Study GroupLars Bode3Jayne S. Danska4Emma Allen-Vercoe5Family Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CORE), the Human Milk Institute (HMI), Department of Pediatrics, School of Medicine, University of California San DiegoFamily Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CORE), the Human Milk Institute (HMI), Department of Pediatrics, School of Medicine, University of California San DiegoResearch Programs Unit, Medical Faculty, University of HelsinkiFamily Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence (MOMI CORE), the Human Milk Institute (HMI), Department of Pediatrics, School of Medicine, University of California San DiegoThe Hospital for Sick ChildrenDepartment of Molecular and Cellular Biology, College of Biological Sciences, University of GuelphAbstract Background Synthetic human milk oligosaccharides (HMOs) are used to supplement infant formula despite limited understanding of their impact on the post-weaned developing gut microbiota. Here, we assess the influence of 0.5 g/L 2-fucosyllactose (2’FL) and 4.0 g/L pooled HMOs (pHMOs) on the composition and activity of cultured fecal-derived microbial communities from seven healthy young children. Results Exposure to pHMOs induced significant shifts in both the microbial community composition and metabolic output, including an increased abundance of several genera, notably Bacteroides, and the production of health-associated metabolites. In contrast, 2’FL alone did not lead to substantial changes in the communities. A total of 330 bacterial isolates, spanning 157 species, were cultured from these communities and individually evaluated for their responses to HMOs. Over 100 non-Bifidobacterium species showed enhanced growth upon pHMOs treatment and a high degree of intraspecies variation in HMO metabolism was observed. Conclusion Our study provides valuable insight into the health-enhancing properties of HMOs while highlighting the need for future research into the efficacy of incorporating individual structures into infant formula, particularly when aiming to modulate the gut microbiota. Video Abstracthttps://doi.org/10.1186/s40168-025-02034-9Child gut microbiotaDeveloping gut microbiotaHuman milkHuman milk oligosaccharides2’-fucosyllactoseMetabolomics |
| spellingShingle | Simone Renwick Annalee Furst Mikael Knip on behalf of the DIABIMMUNE Study Group Lars Bode Jayne S. Danska Emma Allen-Vercoe Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides Microbiome Child gut microbiota Developing gut microbiota Human milk Human milk oligosaccharides 2’-fucosyllactose Metabolomics |
| title | Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides |
| title_full | Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides |
| title_fullStr | Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides |
| title_full_unstemmed | Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides |
| title_short | Modulating the developing gut microbiota with 2’-fucosyllactose and pooled human milk oligosaccharides |
| title_sort | modulating the developing gut microbiota with 2 fucosyllactose and pooled human milk oligosaccharides |
| topic | Child gut microbiota Developing gut microbiota Human milk Human milk oligosaccharides 2’-fucosyllactose Metabolomics |
| url | https://doi.org/10.1186/s40168-025-02034-9 |
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