The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining

ABSTRACT Aims and Objectives Honey possesses specific anti‐inflammatory properties. We evaluated the effects of oral honey supplementation on skeletal muscle‐related inflammatory markers among military graduates after overtraining. Methods This randomized double‐blind controlled trial was conducted...

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Main Authors: Esmail Karami, Mohammad Reza Parvizi, Mohammad Reza Izadi, Emad Jowhari Shirazi
Format: Article
Language:English
Published: Wiley 2025-02-01
Series:Health Science Reports
Subjects:
Online Access:https://doi.org/10.1002/hsr2.70428
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author Esmail Karami
Mohammad Reza Parvizi
Mohammad Reza Izadi
Emad Jowhari Shirazi
author_facet Esmail Karami
Mohammad Reza Parvizi
Mohammad Reza Izadi
Emad Jowhari Shirazi
author_sort Esmail Karami
collection DOAJ
description ABSTRACT Aims and Objectives Honey possesses specific anti‐inflammatory properties. We evaluated the effects of oral honey supplementation on skeletal muscle‐related inflammatory markers among military graduates after overtraining. Methods This randomized double‐blind controlled trial was conducted on 42 overtrained military graduates who were randomly assigned to receive either honey supplementation (5 mL of 12% honey solution twice daily for 6 weeks) or a placebo for 6 weeks. In our study, we utilized Milk Vetch Sahand honey as the intervention. Serum levels of C‐reactive protein (CRP), tumor necrosis factor‐α (TNF‐α), aldolase A, and creatine kinase (CK) were evaluated using the enzyme‐linked immunosorbent assay method before and after the intervention. Results A total of 21 male participants with a mean age of 20.75 years were included in each group. No significant differences were found between the groups in the pre‐intervention assessment of inflammatory biomarkers. However, in the post‐intervention assessment, participants who received honey exhibited significantly smaller increases in CRP (p < 0.001), TNF‐α (p = 0.001), aldolase A (p < 0.001), and CK levels (p < 0.001) compared to the controls. The mean changes in these biomarkers were notably lower in the honey group indicating a potential anti‐inflammatory effect of honey. Conclusion This study demonstrates that honey supplementation significantly reduces inflammatory biomarkers such as TNF‐α, CRP, aldolase A, and CK in military graduates experiencing overtraining, underscoring its potential to alleviate inflammation associated with intense physical training. To confirm and extend these findings, further research with larger sample sizes and longer follow‐up periods is recommended.
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spelling doaj-art-217fa822fa3e4636b9df3f6769d954452025-08-20T02:11:08ZengWileyHealth Science Reports2398-88352025-02-0182n/an/a10.1002/hsr2.70428The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After OvertrainingEsmail Karami0Mohammad Reza Parvizi1Mohammad Reza Izadi2Emad Jowhari Shirazi3Cognitive Neuroscience Research Center, AJA University of Medical Sciences Tehran IranPhysiology Department AJA University of Medical Sciences Tehran IranDepartment of Physical Education and Sports Sciences Imam Hossein Comprehensive University Tehran IranGeneral Practitioner, Student Research Committee AJA University of Medical Sciences Tehran IranABSTRACT Aims and Objectives Honey possesses specific anti‐inflammatory properties. We evaluated the effects of oral honey supplementation on skeletal muscle‐related inflammatory markers among military graduates after overtraining. Methods This randomized double‐blind controlled trial was conducted on 42 overtrained military graduates who were randomly assigned to receive either honey supplementation (5 mL of 12% honey solution twice daily for 6 weeks) or a placebo for 6 weeks. In our study, we utilized Milk Vetch Sahand honey as the intervention. Serum levels of C‐reactive protein (CRP), tumor necrosis factor‐α (TNF‐α), aldolase A, and creatine kinase (CK) were evaluated using the enzyme‐linked immunosorbent assay method before and after the intervention. Results A total of 21 male participants with a mean age of 20.75 years were included in each group. No significant differences were found between the groups in the pre‐intervention assessment of inflammatory biomarkers. However, in the post‐intervention assessment, participants who received honey exhibited significantly smaller increases in CRP (p < 0.001), TNF‐α (p = 0.001), aldolase A (p < 0.001), and CK levels (p < 0.001) compared to the controls. The mean changes in these biomarkers were notably lower in the honey group indicating a potential anti‐inflammatory effect of honey. Conclusion This study demonstrates that honey supplementation significantly reduces inflammatory biomarkers such as TNF‐α, CRP, aldolase A, and CK in military graduates experiencing overtraining, underscoring its potential to alleviate inflammation associated with intense physical training. To confirm and extend these findings, further research with larger sample sizes and longer follow‐up periods is recommended.https://doi.org/10.1002/hsr2.70428honey supplementationinflammatory markersmilitary graduatesovertraining
spellingShingle Esmail Karami
Mohammad Reza Parvizi
Mohammad Reza Izadi
Emad Jowhari Shirazi
The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
Health Science Reports
honey supplementation
inflammatory markers
military graduates
overtraining
title The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
title_full The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
title_fullStr The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
title_full_unstemmed The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
title_short The Effect of Honey Supplementation on Skeletal Muscle‐Related Inflammatory Markers Among Military Graduates After Overtraining
title_sort effect of honey supplementation on skeletal muscle related inflammatory markers among military graduates after overtraining
topic honey supplementation
inflammatory markers
military graduates
overtraining
url https://doi.org/10.1002/hsr2.70428
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