Associations of aged neutrophils with disease activity in primary Sjögren's syndrome

Abstract Background Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease that primarily affects exocrine glands. This study aimed to investigate the correlation between aged neutrophils and disease activity in patients with pSS. Methods This study included 21 patients diagnosed with...

Full description

Saved in:
Bibliographic Details
Main Authors: Lei Han, Chunyuan Xiao, Tianlin Lu, Xinfang Huang
Format: Article
Language:English
Published: Wiley 2025-03-01
Series:Rheumatology & Autoimmunity
Subjects:
Online Access:https://doi.org/10.1002/rai2.12162
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849390948510859264
author Lei Han
Chunyuan Xiao
Tianlin Lu
Xinfang Huang
author_facet Lei Han
Chunyuan Xiao
Tianlin Lu
Xinfang Huang
author_sort Lei Han
collection DOAJ
description Abstract Background Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease that primarily affects exocrine glands. This study aimed to investigate the correlation between aged neutrophils and disease activity in patients with pSS. Methods This study included 21 patients diagnosed with pSS and 32 healthy controls (HCs) at Shanghai East Hospital between September 2021 and May 2023. Peripheral blood samples were collected from them. Neutrophil subsets were identified using cell surface markers (CD62L, CXCR4, CD11b, and CXCR1) and analyzed using flow cytometry. Clinical data, including the European League Against Rheumatism Sjögren's Syndrome Disease Activity Index (ESSDAI) and serum ferritin levels, were also collected at admission. Results Serum ferritin levels were associated with the ESSDAI and neutrophil‐to‐lymphocyte ratio in patients with pSS. Our study revealed a significant increase in the frequency of aged neutrophils in patients with pSS compared to HCs (p < 0.05). The frequency of aged neutrophils significantly correlated with ESSDAI and ferritin levels in patients with pSS (p < 0.05). We generated a receiver operating characteristic (ROC) curve to evaluate the diagnostic utility of aged neutrophils in pSS. ROC analysis showed an area under the curve of 0.8036, with a p‐value of 0.0002. Conclusions This study confirmed that patients with pSS exhibit a higher percentage of aged neutrophils. Furthermore, aged neutrophils may serve as diagnostic markers of pSS.
format Article
id doaj-art-217f0546b4674e42b301bf4289e6d6db
institution Kabale University
issn 2767-1410
2767-1429
language English
publishDate 2025-03-01
publisher Wiley
record_format Article
series Rheumatology & Autoimmunity
spelling doaj-art-217f0546b4674e42b301bf4289e6d6db2025-08-20T03:41:15ZengWileyRheumatology & Autoimmunity2767-14102767-14292025-03-0151586310.1002/rai2.12162Associations of aged neutrophils with disease activity in primary Sjögren's syndromeLei Han0Chunyuan Xiao1Tianlin Lu2Xinfang Huang3Department of Rheumatology, Shanghai East Hospital Tongji University School of Medicine Shanghai ChinaDepartment of Rheumatology, Shanghai East Hospital Tongji University School of Medicine Shanghai ChinaDepartment of Rheumatology, Shanghai East Hospital Tongji University School of Medicine Shanghai ChinaDepartment of Rheumatology, Shanghai East Hospital Tongji University School of Medicine Shanghai ChinaAbstract Background Primary Sjögren's syndrome (pSS) is a chronic autoimmune disease that primarily affects exocrine glands. This study aimed to investigate the correlation between aged neutrophils and disease activity in patients with pSS. Methods This study included 21 patients diagnosed with pSS and 32 healthy controls (HCs) at Shanghai East Hospital between September 2021 and May 2023. Peripheral blood samples were collected from them. Neutrophil subsets were identified using cell surface markers (CD62L, CXCR4, CD11b, and CXCR1) and analyzed using flow cytometry. Clinical data, including the European League Against Rheumatism Sjögren's Syndrome Disease Activity Index (ESSDAI) and serum ferritin levels, were also collected at admission. Results Serum ferritin levels were associated with the ESSDAI and neutrophil‐to‐lymphocyte ratio in patients with pSS. Our study revealed a significant increase in the frequency of aged neutrophils in patients with pSS compared to HCs (p < 0.05). The frequency of aged neutrophils significantly correlated with ESSDAI and ferritin levels in patients with pSS (p < 0.05). We generated a receiver operating characteristic (ROC) curve to evaluate the diagnostic utility of aged neutrophils in pSS. ROC analysis showed an area under the curve of 0.8036, with a p‐value of 0.0002. Conclusions This study confirmed that patients with pSS exhibit a higher percentage of aged neutrophils. Furthermore, aged neutrophils may serve as diagnostic markers of pSS.https://doi.org/10.1002/rai2.12162aged neutrophilESSDAIprimary Sjögren's syndrome
spellingShingle Lei Han
Chunyuan Xiao
Tianlin Lu
Xinfang Huang
Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
Rheumatology & Autoimmunity
aged neutrophil
ESSDAI
primary Sjögren's syndrome
title Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
title_full Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
title_fullStr Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
title_full_unstemmed Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
title_short Associations of aged neutrophils with disease activity in primary Sjögren's syndrome
title_sort associations of aged neutrophils with disease activity in primary sjogren s syndrome
topic aged neutrophil
ESSDAI
primary Sjögren's syndrome
url https://doi.org/10.1002/rai2.12162
work_keys_str_mv AT leihan associationsofagedneutrophilswithdiseaseactivityinprimarysjogrenssyndrome
AT chunyuanxiao associationsofagedneutrophilswithdiseaseactivityinprimarysjogrenssyndrome
AT tianlinlu associationsofagedneutrophilswithdiseaseactivityinprimarysjogrenssyndrome
AT xinfanghuang associationsofagedneutrophilswithdiseaseactivityinprimarysjogrenssyndrome