CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy
Objective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/2743614 |
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| author | Jan Jiskra Marie Antošová Jan Krátký Hana Vítková Zdeňka Límanová Helena Marečková Eliška Potluková |
| author_facet | Jan Jiskra Marie Antošová Jan Krátký Hana Vítková Zdeňka Límanová Helena Marečková Eliška Potluková |
| author_sort | Jan Jiskra |
| collection | DOAJ |
| description | Objective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood. Results. The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, p=0.008) and in peripheral blood (6.7 versus 5.13%, p=0.047), and positively correlated with serum antibodies to thyroid peroxidase (r=0.243; p=0.026) and negatively correlated with thyroid volume (r=-0.346; p=0.008). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones. Conclusion. Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls. |
| format | Article |
| id | doaj-art-217ecfb29b244a85bd5f2ea0a7fe4999 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-217ecfb29b244a85bd5f2ea0a7fe49992025-02-03T01:02:05ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/27436142743614CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration BiopsyJan Jiskra0Marie Antošová1Jan Krátký2Hana Vítková3Zdeňka Límanová4Helena Marečková5Eliška Potluková6Third Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicThird Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicThird Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicThird Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicThird Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicInstitute of Medical Biochemistry and Laboratory Diagnostics, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicThird Medical Department, The First Faculty of Medicine, Charles University and The General University Hospital in Prague, 128 08 Prague, Czech RepublicObjective. To determine the expression of chemokine receptors in lymphocytes from thyroid nodules and peripheral blood in patients with and without Hashimoto’s thyroiditis (HT). Patients and Methods. The study included 46 women with thyroid nodules and HT and 60 women with thyroid nodules without HT (controls) who underwent a fine needle aspiration biopsy (FNAB). Expression of chemokine receptors CXCR3, CCR5, and CRTH2 was assessed by flow cytometry in lymphocytes from FNAB samples and from peripheral blood. Results. The percentage of CRTH2+ lymphocytes was higher in nodules with HT in comparison with controls, both in FNAB samples (13.95 versus 6.7%, p=0.008) and in peripheral blood (6.7 versus 5.13%, p=0.047), and positively correlated with serum antibodies to thyroid peroxidase (r=0.243; p=0.026) and negatively correlated with thyroid volume (r=-0.346; p=0.008). Lymphocytes from neoplastic nodules showed a higher expression of both CXCR3 and CCR5 than those from hyperplastic ones. Conclusion. Flow cytometry performed in FNAB samples may serve as a good tool in investigation of intrathyroidal expression of immunological parameters. In our study, the CRTH2 expression on thyroid-infiltrating lymphocytes as well as on lymphocytes from peripheral blood was increased in HT as compared to controls.http://dx.doi.org/10.1155/2016/2743614 |
| spellingShingle | Jan Jiskra Marie Antošová Jan Krátký Hana Vítková Zdeňka Límanová Helena Marečková Eliška Potluková CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy Journal of Immunology Research |
| title | CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy |
| title_full | CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy |
| title_fullStr | CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy |
| title_full_unstemmed | CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy |
| title_short | CXCR3, CCR5, and CRTH2 Chemokine Receptor Expression in Lymphocytes Infiltrating Thyroid Nodules with Coincident Hashimoto’s Thyroiditis Obtained by Fine Needle Aspiration Biopsy |
| title_sort | cxcr3 ccr5 and crth2 chemokine receptor expression in lymphocytes infiltrating thyroid nodules with coincident hashimoto s thyroiditis obtained by fine needle aspiration biopsy |
| url | http://dx.doi.org/10.1155/2016/2743614 |
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